Jump to content
Sign in to follow this  
Guest guest

Drug Resistance-Associated Genotypic Alterations in ART-Naives in North India

Rate this topic

Recommended Posts

Guest guest

Drug Resistance-Associated Genotypic Alterations in the pol Gene of

HIV Type 1 Isolates in ART-Naive Individuals in North India


To cite this paper:

Sunil K. Arora, Sachin Gupta, Jaideep S. Toor, Anuj Singla. AIDS

Research and Human Retroviruses. February 1, 2008, 24(2): 125-130.



Department of Immunopathology, Postgraduate Institute of Medical

Education and Research, Chandigarh, India.


We genotyped the RT and PI regions of the pol gene of HIV-1 from

treatment-naive infected individuals in North India and evaluated

their possible physiological relevance and association with drug


Plasma samples from 52 newly diagnosed HIV-1-infected drug-naive

individuals were subjected to CD4+ cell count and plasma viral load.

For genotyping, the protease and RT regions of the pol gene were

amplified from cDNA reverse transcribed from plasma viral RNA by

single or nested polymerase chain reaction (PCR). Sequences of

amplified products were analyzed for mutations using the Stanford DR

and REGA database.

Two out of 49 amplicons showed mutations at known " major " subtype B

drug resistance positions (one each in protease and RT). In the

protease region it showed a major drug resistance mutation at M46I as

well as " minor " positions F53L and T74P. In the RT gene, one patient

showed a mutation at major NNRTI position G190V. Forty-nine percent

had mutations in the hinge (M36I, R41K, H69K) and รก-helix (L89M)

regions of the C-virus protease, which has been linked to increased

catalytic activity. Our study indicates that a number of major

mutations associated with resistance to PIs, NNRTIs, and NRTIs do

exist, though at a low frequency, among HIV-1 isolates from treatment-

naive individuals in North India.

Many minor or accessory mutations related to resistance to PIs and

NRTIs are also present as the variants. These results point to the

greater biochemical fitness of subtype C protease and faster decrease

in drug sensitivity.

Share this post

Link to post
Share on other sites

Join the conversation

You are posting as a guest. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.

Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.

Sign in to follow this  

  • Create New...