some PCR questions En-DE

[Guest guest]

Dear Listmates

I have a problem understanding the following sentence:

The probe position and the primer binding site overlap, leading to any

excess primer in the amplicon binding to the strip.

Also what is the German expression for

core allele

Thaks for any input

Kind regards

Judy Ann

Dipl. Phys. Judy Ann Schoen

EDV & Seminare - Neue Seminartermine im Juni und Juli

**Atril's Certified Training Partner**

Tölzer Str. 12

83661 Lenggries

Tel. 08042 973197

Fax. 08042 973198

www.edv-und-seminare.de

mailto:info@...

[Guest guest]

Hi Judy Ann,

might Hauptallele work in your context?

Dee

JAS wrote:

> Dear Listmates

> I have a problem understanding the following sentence:

>

> The probe position and the primer binding site overlap, leading to any

> excess primer in the amplicon binding to the strip.

>

> Also what is the German expression for

> core allele

>

> Thaks for any input

>

> Kind regards

> Judy Ann

>

> Dipl. Phys. Judy Ann Schoen

> EDV & Seminare - Neue Seminartermine im Juni und Juli

> **Atril's Certified Training Partner**

> Tölzer Str. 12

> 83661 Lenggries

> Tel. 08042 973197

> Fax. 08042 973198

> www.edv-und-seminare.de

> mailto:info@...

>

>

>

[Guest guest]

Hi Judy Ann:

perhaps it helps breaking up the sentence as follows:

----- Original Message -----

I have a problem understanding the following sentence:

The probe position - and - the primer binding site - overlap, leading to

any

excess primer - in the amplicon - binding to the strip.

Also what is the German expression for

core allele

---> that might depend on the context:

= main allele? das Hauptallel

= inner allele (of a string of amplified alleles)? das innere Allel,

Innenallel, Kernallel?

(just some suggestions... I realy don't know without digging deaper)

Thaks for any input