Effect of myostatin on turkey myogenic satellite cells and embryonic myoblasts

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Comp Biochem Physiol A Mol Integr Physiol. 2006 Apr 30

Effect of myostatin on turkey myogenic satellite cells and embryonic

myoblasts.

McFarland DC, Velleman SG, Pesall JE, Liu C.

Department of Animal and Range Sciences, Box 2170, ASC 101, South

Dakota State University, Brookings, SD 57007-0392, USA.

Myostatin (GDF-8) inhibits the activation, proliferation, and

differentiation of myogenic satellite cells. The relative importance

of this growth factor is demonstrated in myostatin-null mice and

cattle possessing defective myostatin genes. These defects result in

greatly enhanced musculature. In the present study, we examined the

effect of myostatin on turkey myogenic satellite cells and embryonic

myoblasts. Compared with controls (P<0.05), proliferation of both

turkey embryonic myoblasts and satellite cells was inhibited between

26 and 45% in serum-free medium containing 20 ng/mL myostatin. While

individual turkey satellite cell clones differed in their

responsiveness to myostatin, there were no significant differences in

the responsiveness of fast and slow growing cells as groups (P>0.05).

A slow growing clone that exhibited the greatest response to

myostatin also exhibited the greatest depression of differentiation

with this growth factor (P<0.05). All other turkey satellite cell

clones exhibited similar responses to the differentiation depressing

effects of myostatin (P>0.05). However, myostatin had no effect on

differentiation of turkey embryonic myoblasts (P>0.05). When exposed

to myostatin, 4 of 6 proliferating clones and all differentiating

clones increased their expression of decorin, a growth inhibitor

(P<0.05). The present study demonstrates that myostatin inhibits the

proliferation and differentiation of satellite cells and suggests a

role for decorin in myostatin action in muscle development.