Influence of Vit C + E on Oxidative Stress induced by Hyperbaric Oxygen in men

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Ann Nutr Metab. 2006 Jan 10;50(3):173-176

Influence of Vitamin C and E Supplementation on Oxidative Stress

Induced by Hyperbaric Oxygen in Healthy Men.

Bader N, Bosy-Westphal A, Koch A, Mueller MJ.

Institute of Human Nutrition and Food Science, Christian-Albrechts-

Universitat zu Kiel, Kiel, Germany.

Aim: To investigate the effect of a 4-week vitamin C and E

supplementation on oxidative stress induced by hyperbaric oxygen

(HBO). Methods: 19 healthy men were exposed to 3 sequential

protocols, i.e. HBO (100% O(2), 2.4 bar, 131 min) before (T1) and

after 4 weeks of daily supplementation with 500 mg slow-release

vitamin C and 272 IU vitamin E (T2). A normoatmospheric protocol (21%

O(2), 1.0 bar, 131 min) served as control treatment (nonexposed).

Blood samples were taken before ( and immediately after (A)

treatment.

Plasma levels of vitamin A, C, E, beta-carotene, reduced glutathione

and malondialdehyde were measured by HPLC. Antioxidative capacity and

lipid peroxides in plasma were analyzed by ELISA. Results: HBO

decreased vitamin C and antioxidative capacity (T1). At T1, Delta A -

B of vitamin C and lipid peroxides was different from nonexposed.

Vitamin supplementation increased plasma levels of vitamin C and E by

28 and 37%, respectively. Vitamin supplementation led to decreased

concentrations of lipid peroxides and reduced glutathione. After

supplementation, HBO decreased vitamin C and reduced glutathione. At

T2, Delta A - B of vitamin C and lipid peroxides was significantly

different from nonexposed.

Conclusion: In humans, oxidative stress decreased plasma levels of

vitamin C and antioxidative capacity and increased plasma lipid

peroxides. Supplementation with vitamin C and E did not prevent these

effects.