Disinfecting and sporocidal composition and process for decontaminating buildin

[Guest guest]

I found this formulation on a website called freepatentsonline.com. I

guess it means anyone can use it without paying the author. The

formulation is based on hydrogen peroxide, t-butyl hydroperoxide,

propylene glycol and quaternary ammonium salts. It is argued that it

can destroy spores of Stachy AND its mycotoxins below 0,1 ppm. That's

interesting to say the least.

http://www.freepatentsonline.com/6500465.html

Disinfecting and sporocidal composition and process for

decontaminating buildings

United States Patent 6500465

Abstract: The present invention relates to a volatile, residue free

peroxide antimicrobial composition, which can be applied as a

penetrating and durable, fine aerosol, that has superior strength with

respect to decontaminating buildings infected with bacteria, fungi,

virus or fungal or bacterial spores. The present invention is also

directed to a process for decontaminating large man made structures

and air contained in these.

Claims:

I claim:

1. A liquid biocidal, deodorant and mycotoxin and/or endotoxin

denaturing composition, comprising:

(a) 0.5-60 wt % of hydrogen peroxide;

( 0.5-60 wt % of t-butyl hydroperoxide;

© 5-90 wt % of a water compatible glycol or glycol ether; and

(d) 0-89 wt % of water.

said composition being characterized by its lack of residue and being

convertible, utilizing thermofogging techniques, into a durable, high

density, fine aerosol with effective antimicrobial, deodorizing and

toxin denaturing activity.

2. A composition according to claim 1, wherein the molar ratio between

hydrogen peroxide and t-butyl hydroperoxide is between 1:10 and 10:1

and the total peroxide concentration is between 0.5 and 30% by weight.

3. A composition according to claim 2, wherein the molar ratio between

hydrogen peroxide and t-butyl hydroperoxide is between 1:3 and 3:1 and

the total peroxide concentration is between 5 and 20% by weight.

4. A composition according to claim 1 where the glycol is propylene

glycol and the total propylene glycol concentration is between 10 and

90% by weight.

5. A composition according to claim 4 where the propylene glycol

concentration is between 20 and 70% by weight.

6. A composition according to claim 1, which additionally includes up

to 10% by weight of a surfactant.

7. A composition according to claim 4, wherein the surfactant is a

quaternary ammonium compound.

8. A composition according to claim 7, wherein the surfactant is

didecyl dimethyl ammonium chloride.

9. A method of disinfecting and deodorizing and/or denaturing myco-

and/or endotoxins which comprises applying an antimicrobially

effective amount of the solution claimed in claim 1 in the form of a

durable, high density, fine aerosol to a surface, object or air space

requiring disinfecting and/or deodorizing and/or myco- and/or

endotoxin denaturing.

Description:

TECHNICAL FIELD

The present invention relates to a volatile, residue free peroxide

antimicrobial composition, which can be applied as a penetrating and

durable, fine aerosol, that has superior strength with respect to

decontaminating buildings infected with bacteria, fungi, virus or

fungal or bacterial spores.

The present invention is also directed to a process for

decontaminating large man made structures and the air contained in these.

BACKGROUND OF THE INVENTION

Disinfecting biologically contaminated large man-made structures--as

for example shopping malls, sports complexes, high rises, subway

systems, factories, etc.--as well as the air contained in these

structures is an extremely challenging undertaking and none of the

presently available methods are satisfactory.

The most widely used method is fumigation with formaldehyde.

Formaldehyde is a suspected carcinogenic and a potent allergen which,

due to inevitable residues left after a treatment, severely limits its

usefulness in structures inhabited by man.

Numerous attempts have been made to use oxidizing gases such as ozone

or chlorine dioxide for decontaminating large buildings. However, the

results have invariably been very disappointing. This is to some

extent due to the inherent inability of gases to penetrate a porous

structure within a reasonable time. In fine pores diffusion is the

only way for a gas to spread, and this process is slow. Mainly,

though, the failure of ozone and chlorine dioxide in building

decontamination is due to the instability and extreme reactivity of

these gases. They are very toxic to man and will also corrode

virtually any oxidizeable material, (metals, wood, textiles, plants,

plastics, etc.) Actually the major part of these gases will be

consumed in unwanted oxidation reactions, that cause collateral

damage, and for health and safety reasons is basically not possible to

apply these gases at the levels required for efficient decontamination

to take place.

From a health and environmental point of view disinfecting agents

based on peroxides, such as hydrogen peroxide, peracetic acid and

like, is much to be preferred. Their oxidizing strength, without being

excessive, in principle is adequate for killing virtually all

microbes. Unfortunately hydrogen peroxide or other peroxides are too

unstable and hazardous to allow fumigating with their vapors.

High density, fine aerosols (aerosol droplet diameter less than 50

micron) of aqueous peracetic acid, hydrogen peroxide or combinations

thereof, suitable for disinfecting are only sufficiently stable at

100% R.H., and the commercial fog-disinfecting methods using peroxides

are only suitable for confined spaces where all materials and

equipment are corrosion resistant or protected.

SUMMARY DISCLOSURE OF INVENTION

The objective of said invention is to provide an antimicrobial

composition that can be applied at ambient conditions as a high

density, durable fine aerosol having superior disinfecting strength

with respect to microbes and spores thereof adhered to inanimate

surfaces as well as with respect to airborne microbes and microbial

spores, such an aerosol being imminently suitable for emergency

decontamination of buildings and spaces infected with hazardous

microbes or spores thereof.

As a result of extensive research, I have found that a mixture of

hydrogen peroxide and tert-butyl hydroperoxide in propylene glycol or

other water compatible glycols can be converted to a high density,

durable fine aerosol, using conventional thermofogging equipment (see

below), without appreciable loss of peroxide activity through thermal

decomposition, and that such aerosols manifest unexpected high

disinfecting strength with respect to microorganisms including fungal

and bacterial spores without causing staining, corrosion or irritating

odors. The use of a combination of hydrogen peroxide and tert-butyl

hydroperoxide for bleaching of pulp has been described in U.S. Pat.

No. 3,645,840. However, it is by no means obvious how the disclosures

of U.S. Pat. No. 3,645,840 can be applied to an aerosol process for

disinfecting buildings. U.S. Pat. No. 5,147,884 describes

antimicrobial composition containing tert-butyl hydroperoxide and a

monophenylglycol ether.

In the disinfecting method according to this invention the peroxide

antimicrobial agent is converted into a fine aerosol using various

types of " thermofoggers " , such as for example a pulsejet fogger or an

electrical fogger with a flash heating system. Examples of pulsejet

thermofoggers are " Patriot " and " Black Hawk " manufactured by Curtis

Dynafog Corporation, Ltd., Indianapolis, and an example of a suitable

electrical thermofogger is " Fogmax " manufactured by CITC, Lynnwood,

Wash. Normally the high temperature in thermofoggers will destroy any

peroxide. However, as mentioned above, I have discovered combinations

of peroxides and carriers that can resist the high temperatures

encountered in thermofoggers, making this ideal aerosol technique

available to generate fine, high density, durable aerosols of

peroxides. The fine aerosol produced in this manner will spread much

like a gas, which makes it ideal for decontamination of large

structures as well as inaccessible areas of buildings. However, in

contrast to a gaseous agent, once an aerosol according to this

invention has settled on a surface, it behaves like a liquid, that is,

the agent can be transported by capillary action deep into a porous

material, which is impossible with ozone or chlorine dioxide. The

peroxide disinfecting method according to this invention kills mold,

bacteria and fungi as well as spores thereof. The same oxidation

reaction also degrades it and neutralizes the odor compounds from

mold, fungi and bacteria (MVOC).

Another advantage of the disinfecting method according to this

invention is that airborne microbes are disinfected with the same

efficiency as those adhered to surfaces. Furthermore airborne

particulate matter is removed from treated air spaces through

agglomeration with the aerosol droplets, leaving the treated air

virtually free from microbes and their spores.

The present invention provides a safe and effective method of

sanitizing surfaces and ambient air by removing, reducing or retarding

the growth of pathogenic microorganisms and molds without the use of

substances that are toxic to humans and without leaving any permanent

residue.

BEST MODE FOR CARRYING OUT THE INVENTION

As the peroxide used as component (A) of said invention, commercially

available hydrogen peroxide aqueous solutions can be used favorably.

Next, the volatile, organic peroxide used as component (, is

commercially available t-butyl hydroperoxide.

The content of the hydrogen peroxide used as component (A) in the

biocidal composition is generally 0.5-60 wt %, preferably 0.5-30 wt %,

and more preferably 0.5-20 wt %. For practicality, 3-20 wt % is most

favorable. The content of t-butyl hydroperoxide, which is component

(, is 0.5-60 wt %, preferably 0.5-30 wt %, and more preferably

0.5-10 wt %. For practicality, 3-10 wt % is most favorable.

If component (A) or ( is lower than said range, the disinfecting

action is low, when component (A) or ( is greater than said range,

the product becomes difficult to handle as a biocidal composition.

The solvent carrier © of the biocidal composition in said invention

is important for thermal stability, for aerosol forming properties as

well as for obtaining high disinfecting strength. After extensive

research I have found that mixtures of water and a low volatile, water

compatible glycol or glycol ether are preferable, and most preferable

are mixtures of propylene glycol and water where the content of

propylene glycol in water is 10-90 wt %.

The biocidal composition of the present invention is normally

manufactured by dissolving hydrogen peroxide (A) and t-butyl

hydroperoxide ( in a mixture of water and propylene glycol ©. The

content of propylene glycol © used in the biocidal composition in

said invention is selected from a range of 5-99 wt %, preferably 50-95

wt %, and more preferably 60-90 wt %.

In the biocidal composition of the present invention, it is preferable

to add a surfactant. As the surfactant, cationic surfactants such as

aryl-alkyl or dialkyl dimethyl ammonium halides, nonionic surfactant

such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid

esters, amine oxides, etc., and anionic surfactants such as soaps,

alkyl sulfates, and alkylbenzenesulfonates, etc., can be utilized. It

is preferable for the quantity of the surfactant added to be 0.1-10 wt

% in the biocidal composition. By the addition of a surfactant, it is

possible to assist in the penetration of the biocidal composition with

respect to the bacterial bio-film, mold or spore coating and to

enhance the biocidal effect.

The biocidal composition of the present invention is applied as a fine

aerosol using thermofogging equipment described above, and it is

possible to disinfect spores, bacterial bio-films or molds effectively

by contacting with said fine aerosol.

By thermal fogging, the biocidal composition of this invention is

dispersed in 10-20 micron particle sizes and the treated building is

kept closed for a minimum of twenty-four (24) hours for treatment to

occur.

No rinsing of treated surfaces is required after or prior to

application of the disinfecting aerosol according to this invention.

Typical bacteria which can be disinfected with the composition of this

invention include: staphylococcus aureus, staphylococcus pyogenes,

streptococcus hemolyticus, streptococcus dysgalactiae, mycobacterium

tuberculosis, salmonella typhosa, salmonella typhimurium, salmonella

pullorum, hemophilus parasuis, clostridium perfringens, mycoplasma

synoviae, mycoplasma hyopneumoniae, pasteurella multocida, klebsiella

pneumoniae, staphylococcus epidermis, streptococcus agalactiae,

streptococcus fecalis, listeria monocytogenis, mycobacterium

tuberculosis, salmonella choleraesuis, salmonella enteritidis,

pseudomonas aeruginosa, clostridium tetani, diplococcus pneumoniae,

mycoplasma gallisepticum, escherichia coli, pasteurella hemolytica,

alcaligenes faecalis, salmonella gallinarum, salmonella arizonae,

salmonella schotimuelleri, staphylococcus hyicus, streptococcus

pyogenes, haemophilus parasuis; and, bordetella bronchiseptica.

Fungus types, which may be disinfected by the composition of this

invention, include: aspergillus fumigatus, aspergillus glacus,

aspergillus nidulans, aspergillus flavus, aspergillus niger, fusarium

solani; and penicillium variable.

Spore types, which may be disinfected by the composition of this

invention, include: Bacillus anthracis, B., and (bacterial spores) and

Stachybotrys, Aspergillus, Penicillium, Trichoderma and Alternaria

spp. (fungal spores).

Viruses which are disinfected by this composition include:

Adenoviridae (Egg Drop Syndrome), Herpetoviridae (Infectious Bovine),

Rhinotracheitis (Aujeszky's Disease), Feline Herpes, Iridoviridae

(African Swine Fever), Parvoviridae, (Canine Parvovirus), Poxviridae

Pseudo (Cowpox), Coronaviridae (Transmissible Gastro-Enteritis), Avian

Infectious Bronchitis, Canine Coronavirus, Orthomyxoviridae (Avian

Influenza), Paramyxoviridae (Newcastle Disease), Distemper,

Picornaviridae (Swine Vesicular Disease), Foot & Mouth Disease,

Reoviridae Gumboro (IBD), Retroviridae (Maedi & Visna), AIDS.

TABLE 1

THERMOFOGGING STABILITY OF PEROXIDE

FORMULATIONS

14% FORMULATION 17%

FORMULTION

10% FORMULTION

COMPONENT 1 2 3 4 5 6 7 8 9

10 11 12 13 14

HYDROGEN PEROXIDE, wt % 14 0 7 7 11 3 17 0

10

10 10 0 6 6

t-BUTYL HYDROPEROXIDE, wt % 0 14 7 7 3 11 0 17 7

7 0 10 4 4

PROPYLENE GLYCOL, wt % 70 70 70 70 70 70 70

70 70

70 70 70 70 70

DIDECYL DIMETHYL AMMONIUM Cl 0 0 0 1 0 0 0 0 0

1 0 0 0 1

WATER, wt % 16 16 16 15 16 16 13

13 13

12 20 20 20 19

% RELATIVE LOSS OF PEROXIDE 50 0 6 4 20 0 59 0 8

6 43 0 3 1

BY THERMOFOGGING R R

R

EVALUATION OF THERMOFOGGING STABILITY OF VARIOUS PEROXIDE FORMULATIONS

The stability of the peroxide disinfectants according to this

invention were determined in the following manner:

Absolute measurement of the peroxide degradition in the thermofogging

process is very difficult due to the uncontrolled

evaporation/condensation processes taking place during and after

aerosolization. For our purpose a relative measurement is suffucient.

Three series of measurements were carried out with varying total

amount of peroxide (14%, 17% and 10% ) and constant amount of

propylene glycol carrier (Table 1). The formulation Examples of Table

1 were loaded into the chemical tank of a pulsejet thermofogger

(Patriot from Curtis Dynafog Corp.) and dispensed as a fine aerosol

(10-20 micron droplet size) into a 15 m.sup.3 cylindrical (diameter 2

m and length 5 m) test chamber in which 3 clean, tared glass petri

dishes (diameter 20 cm) had been placed on the bottom. An amount of

aerosol corresponding to approximately 2 g of peroxide per m.sup.2 was

dispensed. The aerosol was allowed to settle for 30 minutes before the

petri dished were taken out of the chamber. Each dish was weighed and

then rinsed with a total of 50 ml of destined water and the peroxide

content in this extract was determined by titration with thiosulfate.

In each series the value measured for the formulation based on

tert-butyl hydroperoxide alone was taken as the relative value of 0%

degradation of peroxide ®. As can be seen from Table 1, t-butyl

hydroperoxide substantially reduces the thermal decomposition of

hydrogen peroxide in the thermofogging process. This stabilizing

effect of t-butyl hydroperoxide on hydrogen peroxide is even more

pronounced when an electrical fogger is used for aerosol generation.

TABLE 2

APPLICATION EXAMPLES

EXAMPLE No.

COMPONENT, WT% 1 2 3 4

HYDROGEN PEROXIDE 14 0 7 7

t-BUTYL HYDROPEROXIDE 0 14 7 7

PROPYLENE GLYCOL 70 70 70 70

DIDECYL DIMETHYL AMMONIUM CHLORIDE 0 0 0 1

WATER 16 16 16 15

Evaluation of Antimicrobial Efficacy

The exemplary formulations described in Table 2 above were evaluated

for their antimicrobial efficacy against Pseudomonas aeruginosa (ATCC

15442), Escherichia coli (ATCC 10536), Staphylococcus aureus

(gram-positive type pathogenic bacteria) (ATCC 6538), Enterococcus

hirae (ATCC 10541), Salmonella typhimurium (gram-negative type

pathogenic bacteria) (ATCC 13311), Aureobasidium pullulans (black

mold) in the following manner:

Culture Method:

The test organisms described above were transplanted individually to

suitable agar culture media and incubated (at 30-35.degree. C. and 24

hours for the bacteria cultures and 28-30.degree. C. and 20 days for

the mold culture) to develop confluent growth, and the bacterical

(mold) colonies forming units (cfu) was determined (no treatment level).

Treatment Method:

Two Petri dishes of each culture were placed in a chamber with

controlled temperature (25.degree. C.) and humidity (85% R.H.). The

chamber was filled with an aerosol (average droplet size 10 micron) of

the exemplary formulation (Table 2) to be tested using a pulse-jet

fogger (Curtis Dynafog model Patriot). The amount of formulation

applied in all cases corresponded to approximately 20 g of formulation

per square meter culture medium. The chamber was kept closed for 24

hours and the bacterial and mold cfu were determined.

Method for Evaluation of the Biocidal Activity:

The test method for evaluation of the bactericidal activity of a

composition on clean surfaces described in European Standard, EN

1276:1997 issued by the European committee for standardization,

Brussels, was adapted to this test.

European Standard, EN 1276:1997, specifies a test method and

requirements for the minimum bactericidal activity of a disinfecting

composition. The test is passed if the bacterial colonies forming

units (cfu) are reduced from a 10.sup.7 cfu (initial level) to a

10.sup.2 cfu (final level after contact with the disinfecting

product), i.e. a 10.sup.5 reduction of the viability is necessary. The

results obtained for the exemplary compositions in Table 2 are shown

in Table 3. " + " indicates at least a 5 log reduction in cfu and " - "

indicates less than a 5 log reduction in cfu

TABLE 3

EVALUATION OF BIOCIDAL EFFICACY

EXAMPLE

BIOCIDAL EFFICACY

TEST ORGANISM 1 2 3 4

Staphylococcus aureus (ATCC 6538) + - + +

Salmonella cholerasuis (ATCC 10708) + - + +

Pseudomonas aeruginosa (ATCC 15442) - - + +

Aureobasidium pullulans - - + +

Aspergillus flavus - - + +

Penicillium sp. - - + +

Evaluation of Sporocidal Efficacy

The exemplary formulations described in Table 2 above were evaluated

for their sporocidal efficacy against Bacillus globigii, B.

licheniformis and B. subtilis spores (bacterial spores) and against

spores of Aspergillus and Penicillium spp.

Contamination Simulation Method:

A glass contamination chamber with a floor surface area of 0.215

m.sup.2 was placed into a fume hood and outfitted with a HEPA-filtered

ventilation port to relieve excess air during the contamination

process. An aerosol nebulizer was mounted on the interior cover at one

end of the chamber. Various pieces of optical scanning equipment and

several control petri dishes were placed onto the floor of the

chamber. Two milliliters of a 3.0.times.10.sup.9 CFU/mL spore

preparation diluted with 3 mL of sterile ddH.sub.2 O were added to the

reservoir of the aerosol nebulizer. The chamber was sealed and

compressed air was fed into the nebulizer to begin the contamination

process, which lasted approximately 15 minutes. Once the contents of

the reservoir were completely disseminated, a small amount of clean

air was allowed to vent through the nebulizer and into the chamber

overnight to remove excess moisture. The next morning, half of the

petri dishes were removed to serve as controls for the determination

of contamination density. Additionally, selected areas of the test

equipment that were fully exposed to the

Bacillus/Aspergillus/Penicillium dissemination were swabbed to collect

and measure viable spores. The equipment and the remaining petri

dishes were then transferred into the large (8 m.sup.3) chamber and

placed onto a table in the same orientation as used in the

contamination chamber.

Decontamination Method:

Immediately following transfer of the test samples, the chamber was

filled with an aerosol (average droplet size 15 micron) of the

exemplary formulation (Table 2) to be tested using a pulse-jet fogger

(Curtis Dynafog model Patriot). The amount of formulation applied in

all cases corresponded to approximately 20 g of formulation per square

meter of test surface. The chamber was kept closed for 24 hours

whereafter the remaining control petri dishes were removed and the

surfaces of the test equipment were swabbed. All samples were prepared

and cultured to determine the number of surviving spores.

Analysis/Method for Evaluation of the Sporocidal Activity:Spores were

extracted from the control samples by adding 5 mL of sterile ddH.sub.2

O to each plate and thoroughly swabbing the plate with a cotton swab

to release the adsorbed spores. Serial dilutions were performed on

each extract from the control plates to achieve 1:1000, 1:10,000 and

1:100,000 dilutions respectively. A 100 mL aliquot of each control

plate dilution (10.sup.-3, 10.sup.-4, and 10.sup.-5) was plated in

triplicate on tryptic soy agar. For the test equipment samples, each

swab was wetted in 100 mL of ddH.sub.2 O and used to swab the selected

surface(s). The swab tips were clipped into centrifuge tubes where an

additional 1.0 mL of ddH.sub.2 O was added. These extracts were

serially diluted to yield 10.sup.-2, 10.sup.-3, and 10.sup.-4

dilutions respectively. A 100 mL aliquot of each dilution level was

plated in triplicate and incubated overnight at 37.degree. C.

The control petri dishes were extracted and plated in triplicate to

generate data using the standard test procedure.

The test is passed if the bacterial colonies forming units (cfu) are

reduced from a 10.sup.6 cfu/cm.sup.2 (initial level) to a 10.sup.1

cfu/cm.sup.2 (final level after contact with the disinfecting

product), i.e. a 10.sup.5 reduction of the viability is necessary. The

results obtained for the exemplary compositions in Table 2 are shown

in Table 4. " + " indicates at least a 5 log reduction in viable spore

cfu and " - " indicates less than a 5 log reduction in cfu

TABLE 4

EVALUATION OF SPOROCIDAL EFFICACY

EXAMPLE/BIOCIDAL EFFICACY

TEST ORGANISM 1 2 3 4

Bacillus globigii spores - - + +

Bacillus licheniformis spores - - + +

Bacillus subtilis spores - - + +

Aspergilius spp. spores - - + +

Penicillium spp. spores - - + +

Evaluation of Odor Removal Efficacy

Odor Testing Method

10 panelists smelled the odor of the various culture dishes described

above before and after the treatment with the compositions of Table 2.

The odor was evaluated as follows:

+: Most or all panelists do not sense an irritating or bad odor.

0: About half of the panelists sense an irritating or bad odor.

-: Most or all panelists sense an irritating or bad odor.

TABLE 5

EVALUATION OF ODOR REMOVAL EFFICACY.

EXAMPLE/ODOR

REMOVAL EFFICACY

TEST ORGANISM 1 2 3 4

Staphylococcus aureus (ATCC 6538) + + + +

Salmonella cholerasuis (ATCC 10708) + 0 + +

Pseudomonas aeruginosa (ATCC 15442) + + + +

Aureobasidium pullulans 0 - + +

Aspergilius flavus 0 0 + +

Penicillium sp. 0 0 + +

Evaluation of Mycotoxin Denaturing Efficacy

This evaluation was carried out using pieces of gypsum board from a

water damaged home infested with Stachybotrys chartarum. This species

of mold produces macrocyclic trichothecene mycotoxins such as

Verrucarin, Roridin and Satratoxin. Some symptoms germane to exposure

to this mold include: cold/flu symptoms, nose bleeds, burning

sensation, coughing or difficulty breathing, sore throat, diarrhea,

headaches, dizziness, nausea, fatigue, and rash at the point of

contact--especially in areas of heavy perspiration. These mycotoxins

can also affect the appetite center of the brain, often reducing the

appetite of exposed individuals. The presence of trichothecene

mycotoxins was established using a so called Enzyme Linked

Immunosorbant Assay (ELISA, based on a coupling reaction between a

specific mycotoxin and antibodies specific for those mycotoxin). No

attempts were made to make a quantitative determination. A positive

assay indicates a mycotoxin level above 100 parts per billion (0.1 ppm).

Two pieces (100 cm2 each) of mold infested gypsum board was treated

each of the exemplary compositions of Table 2 in the manner described

under " Treatment Method " above. The results are shown in Table 6. " + "

indicates no detection of mycotoxins, and " - " that presence of

mycotoxins was detected.

TABLE 6

EVALUATION OF MYCOTOXIN DENATURING EFFICACY

EXAMPLE/MYCOTOXIN

DENATURING EFFICACY

TEST ORGANISM 1 2 3 4

Stachybotrys chartarum - - + +

Tests have been carried out to establish the virucidal activity of two

of the above composition (3 and 4 in Table 2) in accordance with the

standard test procedures. These tests have shown the effectiveness of

the composition against the following broad spectrum of viruses and

viral infections when applied as described under " Treatment Method "

above, which gave a 4 log reduction in virus titre:

Adenoviridae (Egg Drop Syndrome), Herpetoviridae (Infectious Bovine),

Rhinotracheitis (Aujeszky's Disease), Feline Herpes, Iridoviridae

(African Swine Fever), Parvoviridae, (Canine Parvovirus), Poxviridae

Pseudo (Cowpox), Coronaviridae (Transmissible Gastro-Enteritis), Avian

Infectious Bronchitis, Canine Coronavirus, Orthomyxoviridae (Avian

Influenza), Paramyxoviridae (Newcastle Disease), Distemper,

Picornaviridae (Swine Vesicular Disease), Foot & Mouth Disease,

Reoviridae Gumboro (IBD), Retroviridae (Maedi & Visna), AIDS.

From the foregoing, it is to be understood that the compositions

according to the invention provide excellent and surprising

disinfecting and deodorant benefits to hard surfaces. Such

compositions in accordance with the present inventive teaching are

particularly advantageously used against known pathogenic/nuisance

microorganisms commonly found in indoor environments.

While the invention is susceptible of various modifications and

alternative forms, it is to be understood that specific embodiments

thereof have been shown by way of examples which however are not

intended to limit the invention to the particular forms disclosed; on

the contrary the intention is to cover all modifications, equivalents

and alternatives falling within the scope and spirit of the invention

as expressed in the appended claims.