ARTICLE :biomarkers of exposure to Stachybotrys

[Guest guest]

1: Environ Health Perspect. 2006 Aug;114(8):1221-6.

Mycotoxin adducts on human serum albumin: biomarkers of exposure to

Stachybotrys chartarum.

Yike I, Distler AM, Ziady AG, Dearborn DG.

Department of Pediatrics and Ann Swetland Center for Environmental

Health, Case Western Reserve University, Cleveland, Ohio 44106-4948, USA.

OBJECTIVE: Despite the growing body of evidence showing adverse health

effects from inhalation exposure to the trichothecene-producing mold

Stachybotrys chartarum, controversy remains. Currently, there are no

reliable assays suitable for clinical diagnosis of exposure. We hypothesized

that satratoxin G (SG) -albumin adducts may serve as biomarkers of exposure

to this fungus. DESIGN: We studied the formation of adducts of SG with serum

albumin in vitro using Western blots and mass spectrometry (MS) and searched

for similar adducts formed in vivo using human and animal serum. RESULTS:

Samples of purified human serum albumin that had been incubated with

increasing concentrations of SG showed concentration-dependent albumin bands

in Western blots developed with anti-SG antibodies. MS analysis found that

as many as 10 toxin molecules can be bound in vitro to one albumin molecule.

The sequencing of albumin-adduct tryptic peptides and the analysis of

pronase/aminopeptidase digests demonstrated that lysyl, cysteinyl, and

histidyl residues are involved in the formation of these adducts. Serum

samples from three patients with documented exposure to S. chartarum

similarly revealed lysine-, cysteine-, and histidine-SG adducts after

exhaustive digestion, affinity column enrichment, and MS analysis. These

adducts were also found in the sera from rats exposed to the spores of S.

chartarum in contrast to control human subjects and control animals.

CONCLUSIONS: These data document the occurrence of SG-albumin adducts in

both in vitro experiments and in vivo human and animal exposures to S.

chartarum. Relevance to clinical practice: SG-amino acid adducts may serve

as reliable dosimeter biomarkers for detection of exposure to S. chartarum.

PMID: 16882529 [PubMed - in process]

[Guest guest]

-Thank You, Im sorry, I should of stated the name of it but couldn't

recall it. it was the other one about indoor air detection.sorry if

you went to any trouble on this, thanks again. -- In

, " sylgja " <sylgja@...> wrote:

>

> 1: Environ Health Perspect. 2006 Aug;114(8):1221-6.

>

> Mycotoxin adducts on human serum albumin: biomarkers of exposure to

> Stachybotrys chartarum.

>

> Yike I, Distler AM, Ziady AG, Dearborn DG.

>

>

>

> Department of Pediatrics and Ann Swetland Center for

Environmental

> Health, Case Western Reserve University, Cleveland, Ohio 44106-

4948, USA.

>

>

>

> OBJECTIVE: Despite the growing body of evidence showing adverse

health

> effects from inhalation exposure to the trichothecene-producing mold

> Stachybotrys chartarum, controversy remains. Currently, there are no

> reliable assays suitable for clinical diagnosis of exposure. We

hypothesized

> that satratoxin G (SG) -albumin adducts may serve as biomarkers of

exposure

> to this fungus. DESIGN: We studied the formation of adducts of SG

with serum

> albumin in vitro using Western blots and mass spectrometry (MS) and

searched

> for similar adducts formed in vivo using human and animal serum.

RESULTS:

> Samples of purified human serum albumin that had been incubated with

> increasing concentrations of SG showed concentration-dependent

albumin bands

> in Western blots developed with anti-SG antibodies. MS analysis

found that

> as many as 10 toxin molecules can be bound in vitro to one albumin

molecule.

> The sequencing of albumin-adduct tryptic peptides and the analysis

of

> pronase/aminopeptidase digests demonstrated that lysyl, cysteinyl,

and

> histidyl residues are involved in the formation of these adducts.

Serum

> samples from three patients with documented exposure to S. chartarum

> similarly revealed lysine-, cysteine-, and histidine-SG adducts

after

> exhaustive digestion, affinity column enrichment, and MS analysis.

These

> adducts were also found in the sera from rats exposed to the spores

of S.

> chartarum in contrast to control human subjects and control animals.

> CONCLUSIONS: These data document the occurrence of SG-albumin

adducts in

> both in vitro experiments and in vivo human and animal exposures to

S.

> chartarum. Relevance to clinical practice: SG-amino acid adducts

may serve

> as reliable dosimeter biomarkers for detection of exposure to S.

chartarum.

>

>

>

> PMID: 16882529 [PubMed - in process]

>

>

>

>