Guest guest Posted August 5, 2006 Report Posted August 5, 2006 I just answered my own question regarding the urine test for mycotoxins. Thought I would share what I had just found on the net. You all might know this anyways, but wanted to share. Just now need to find a doctor in Massachusetts to do this test. Darlene Clinical confirmation of trichothecene mycotoxicosis in patient urine. A. Croft, Bonnie M. Jastromski, L. Croft and Henry A. s Abstract : The investigations of four Cases involving mold-contaminated buildings and human reaction to exposure, documents tests of extracted urine containing trichothecene mycotoxins confirming exposure and the diagnosis of mycotoxicosis in humans. In each of four Cases, the urine demonstrated antibiotic activity, sulfuric acid charring, and protein release. Urine was extracted using ethyl acetate 40V/60V[EA]. Extracted mycotoxin spotted on (TLC) displayed color and a range of (rf) between 0.2-0.6 using various solvents. Extract was re-suspended using 50% ethanol V/V to inject mycotoxins into weanling female Sprague-Dawley rats. Degeneration and necrosis of the rat's tissue followed. Koch's Postulates conditions were fulfilled by isolation of the causative agent, the trichothecene mycotoxins and the reproduction of disease. Examination of human tissue within the urine extraction group confirms Koch's Postulates and comparative pathology confirms inhalation Mycotoxicosis, with severe necrosis of the central nervous system and severe scarring within the lungs. Extraction of mycotoxins from human patient urine is a very useful confirmatory test to demonstrate exposure and identify mycotoxicosis. Low concentrations (6%) of sodium hypochlorite were ineffective against the activity of trichothecene mycotoxin. The severity or stages of disease directly correlates the level of exposure or poisoning (Patent Pending). Key words : Trichothecene, Mycotoxicosis, Urine confirmation test, Human pathology. Author Contact : Dr. A. Croft, DVM, Ph.D., 521 Hilltop Drive, Madison, Wisconsin 53711 (USA). Fax : 715-757-9302 E-mail : doccroft@... Quote
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