MB12 helping sleep

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Just came across this today for the folks who have talked about better sleep

with MB12.

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Brain Res. 1998 Jun 8;795(1-2):98-104. Related Articles, Links

Methylcobalamin amplifies melatonin-induced circadian phase shifts by

facilitation of

melatonin synthesis in the rat pineal gland.

Ikeda M, Asai M, Moriya T, Sagara M, Inoue S, Shibata S.

Advanced Research Center for Human Sciences, Waseda University, Saitama, Japan.

msikeda@...

Effects of methylcobalamin (methyl-B12), a putative drug for treating human

circadian

rhythm disorders, on the melatonin-induced circadian phase shifts were examined

in the

rat. An intraperitoneal injection of 1-100 microg/kg melatonin 2-h before the

activity

onset time (CT 10) induced phase advances of free-running activity rhythms in a

dose-

dependent manner (ED50=1.3 microg/kg). Injection of methyl-B12 (500 microg/kg)

prior

to melatonin (1 microg/kg) injection induced larger phase advances than saline

preinjected controls, while the injection of methyl-B12 in combination with

saline did not

induce a phase advance. These results indicate amplification of

melatonin-induced phase

advances by methyl-B12. Pinealectomy abolished the phase alternating effect of

methyl-

B12, suggesting a site of action within the pineal gland. In fact, methyl-B12

significantly

increased the content of melatonin in the pineal collected 2-h after activity

onset (CT 14).

In contrast, no difference in melatonin content was found at CT 10, indicating

that the

effect of methyl-B12 may be gated after the activity onset time when endogenous

melatonin synthesis is known to increase. These results suggest that methyl-B12

amplifies

melatonin-induced phase advances via an increase in melatonin synthesis during

the early

subjective night at a point downstream from the clock regulation. Copyright 1998

Elsevier

Science B.V. All rights reserved.

PMID: 9622603 [PubMed - indexed for MEDLINE]