Resveratrol Useless?

[Guest guest]

It seems that Kaeberlein and Kennedy have largely

discredited Sinclair's work with resveratrol,

demonstrating that the findings in Sinclair's Nature

paper are an artifact of the Biomol Fluor de Lys

assay!

Logan

J Biol Chem. 2005 Apr 29;280(17):17038-45. Epub 2005

Jan 31.

Substrate-specific activation of sirtuins by

resveratrol.

Kaeberlein M, McDonagh T, Heltweg B, Hixon J, Westman

EA, Caldwell SD, Napper A, Curtis R, DiStefano PS,

Fields S, Bedalov A, Kennedy BK.

Department of Genome Sciences, University of

Washington, Seattle, Washington 98195, USA.

Resveratrol, a small molecule found in red wine, is

reported to slow aging in simple eukaryotes and has

been suggested as a potential calorie restriction

mimetic. Resveratrol has also been reported to act as

a sirtuin activator, and this property has been

proposed to account for its anti-aging effects. We

show here that resveratrol is a substrate-specific

activator of yeast Sir2 and human SirT1. In

particular, we observed that, in vitro, resveratrol

enhances binding and deacetylation of peptide

substrates that contain Fluor de Lys, a

non-physiological fluorescent moiety, but has no

effect on binding and deacetylation of acetylated

peptides lacking the fluorophore. Consistent with

these biochemical data we found that in three

different yeast strain backgrounds, resveratrol has no

detectable effect on Sir2 activity in vivo, as

measured by rDNA recombination, transcriptional

silencing near telomeres, and life span. In light of

these findings, the mechanism accounting for putative

longevity effects of resveratrol should be reexamined.

PMID: 15684413 [PubMed - indexed for MEDLINE]

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