CR, fasting-CR, heat shock protein etc.

[Guest guest]

Heat shock proteins are differentially regulated by the style of CR, regular or

intermittent feeding.

Although the below pdf-available paper appeared to represent not particularly

practical results in the title nor abstract, the text informed. The Fig. 1 data

were mentioned only in the methods section of the text, but had what were for me

intriguing results. The Fig. 1 results other than the title are eye-ball data,

below. Intermittent feeding may require more consideration.

Colotti C, Cavallini G, Vitale RL, Donati A, Maltinti M, Del Ry S, Bergamini E,

Giannessi D.

Effects of aging and anti-aging caloric restrictions on carbonyl and heat shock

protein levels and expression.

Biogerontology. 2005;6(6):397-406.

PMID: 16518701

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve & db=pubmed & dopt=Abstra\

ct & list_uids=16518701 & query_hl=1 & itool=pubmed_docsum

.... Young (3-month-old) male Sprague Dawley rats were divided in groups. A group

was

used to assess values at the younger age. Three groups were raised on different

nutritional regimens: rats in the first group were fed ad libitum a standard

Teklad

diet (Harlan Italy) for life (AL); the second and the third groups were on

caloric

restriction and fed 60% the amount of food consumed by AL rats (DR), or were fed

ad

libitum every other day (intermittent feeding, IF) until the age of 24 months.

Body

weight, food consumption and survival are given (Figure 1). ...

[Figure 1. (a) Body weight, ( Food consumption, © Survival of Sprague Dawley

rats fed ad libitum (AL), on caloric restriction diet (DR) and fed ad libitum

every

other day (IF).

==========================================

Feeding Body weight (g) Food consumption (g/day) Percent surviving

==========================================

AL 560 19 61

DR 380 12 72

IF 420 17 85

==========================================

24-month values are provided.]

Table 2. Heat shock protein levels in cardiac tissue of 3-month and AL

24-month-old

rat.

=============================================================

Protein content 3 months AL 24 months

=============================================================

Hsp27 (lg/mg of total proteins) (Mean±SEM)

LV 0.72±0.35 <0.1

RA 2.03±0.68 <0.1

Hsp60 (lg/mg of total proteins) (Mean±SEM)

LV 5.49±0.08* 3.57±0.19

RA 3.71±0.36** 1.13±0.16

Hsp72 (lg/mg of total proteins) (Mean±SEM)

LV 0.55±0.28 0.21±0.06

RA 0.99±0.18*** 0.35±0.07

Hsc70 (lg/mg of total proteins) (Mean±SEM)

LV 6.85±0.29**** 2.80±0.22

RA 11.29±0.44***** 1.03±0.17

=============================================================

LV: Left Ventricle RA: Right Atrium.

*P = 0.007; **P < 0.0001; ***P = 0.0018; ****P < 0.0001; *****P < 0.0001 vs.

corresponding AL 24 months.

Table 3. Heat shock protein levels in cardiac tissue of 24-month-old rats.

=====================================

Group Hsp27 (mean±SEM) (lg/mg) Hsp60 (mean±SEM) (lg/mg) Hsp72 (mean±SEM)

(lg/mg)

Hsc70 (mean±SEM) (lg/mg)

=====================================

AL 24-monthold rat

RA <0.1 1.1±0.1 0.35±0.07# 1.0±0.2##

LV <0.1 3.6±0.2* 0.21±0.06 2.8±0.2**

IF 24-monthold rat

RA <0.1 1.6±0.1 0.18±0.04 3.7±0.5

LV <0.1 3.4±0.2 0.14±0.02 4.6±0.4

DR 24-month old rat

RA <0.1 1.2±0.2 0.74±0.13*** 1.6±0.1¶

LV <0.1 6.0±0.8¶¶ 0.24±0.01 3.5±0.1

=====================================

#P = 0.0449 vs. RA 24 month DR; ##P <0.0001 vs. RA 24 month IF; *P < 0.0001

vs.

LV 24 month DR; **P = 0.0003 vs. LV 24 month IF; ***P = 0.0058 vs. RA 24 month

IF;

¶P <0.0001 vs. RA 24 month IF; ¶¶P < 0.0001 vs. LV 24 month IF.

-- Al Pater, alpater@...

__________________________________________________

[Guest guest]

Hi folks:

Fig 1 in this study Al posted speaks for itself regarding the

benefits of CR or IF versus Ad lib. But, to me at least, the heat

shock protein 'thing' didn't mean much.

Fwiw, it now appears that HSPs are produced in response to stresses

and are beneficial in protecting against the stress. With aging HSPs

are created much less in response to a stress. The data in this

study show very clearly that the decline in production of HSPs is

much reduced in both regular CR and in IF. More so in IF than in

regular CR, despite higher caloric intake in the case of IF.

Rodney.

>

> Heat shock proteins are differentially regulated by the style of

CR, regular or

> intermittent feeding.

>

> Although the below pdf-available paper appeared to represent not

particularly

> practical results in the title nor abstract, the text informed.

The Fig. 1 data

> were mentioned only in the methods section of the text, but had

what were for me

> intriguing results. The Fig. 1 results other than the title are

eye-ball data,

> below. Intermittent feeding may require more consideration.

>

> Colotti C, Cavallini G, Vitale RL, Donati A, Maltinti M, Del Ry S,

Bergamini E,

> Giannessi D.

> Effects of aging and anti-aging caloric restrictions on carbonyl

and heat shock

> protein levels and expression.

> Biogerontology. 2005;6(6):397-406.

> PMID: 16518701

>

> http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?

cmd=Retrieve & db=pubmed & dopt=Abstract & list_uids=16518701 & query_hl=1 & ito

ol=pubmed_docsum

>

> ... Young (3-month-old) male Sprague Dawley rats were divided in

groups. A group was

> used to assess values at the younger age. Three groups were raised

on different

> nutritional regimens: rats in the first group were fed ad libitum a

standard Teklad

> diet (Harlan Italy) for life (AL); the second and the third groups

were on caloric

> restriction and fed 60% the amount of food consumed by AL rats

(DR), or were fed ad

> libitum every other day (intermittent feeding, IF) until the age of

24 months. Body

> weight, food consumption and survival are given (Figure 1). ...

>

> [Figure 1. (a) Body weight, ( Food consumption, © Survival of

Sprague Dawley

> rats fed ad libitum (AL), on caloric restriction diet (DR) and fed

ad libitum every

> other day (IF).

> ==========================================

> Feeding Body weight (g) Food consumption (g/day) Percent surviving

> ==========================================

> AL 560 19 61

> DR 380 12 72

> IF 420 17 85

> ==========================================

> 24-month values are provided.]

>

> Table 2. Heat shock protein levels in cardiac tissue of 3-month and

AL 24-month-old

> rat.

> =============================================================

> Protein content 3 months AL 24 months

> =============================================================

> Hsp27 (lg/mg of total proteins) (Mean±SEM)

> LV 0.72±0.35 <0.1

> RA 2.03±0.68 <0.1

> Hsp60 (lg/mg of total proteins) (Mean±SEM)

> LV 5.49±0.08* 3.57±0.19

> RA 3.71±0.36** 1.13±0.16

> Hsp72 (lg/mg of total proteins) (Mean±SEM)

> LV 0.55±0.28 0.21±0.06

> RA 0.99±0.18*** 0.35±0.07

> Hsc70 (lg/mg of total proteins) (Mean±SEM)

> LV 6.85±0.29**** 2.80±0.22

> RA 11.29±0.44***** 1.03±0.17

> =============================================================

> LV: Left Ventricle RA: Right Atrium.

> *P = 0.007; **P < 0.0001; ***P = 0.0018; ****P < 0.0001; *****P

< 0.0001 vs.

> corresponding AL 24 months.

>

> Table 3. Heat shock protein levels in cardiac tissue of 24-month-

old rats.

> =====================================

> Group Hsp27 (mean±SEM) (lg/mg) Hsp60 (mean±SEM) (lg/mg) Hsp72

(mean±SEM) (lg/mg)

> Hsc70 (mean±SEM) (lg/mg)

> =====================================

> AL 24-monthold rat

> RA <0.1 1.1±0.1 0.35±0.07# 1.0±0.2##

> LV <0.1 3.6±0.2* 0.21±0.06 2.8±0.2**

> IF 24-monthold rat

> RA <0.1 1.6±0.1 0.18±0.04 3.7±0.5

> LV <0.1 3.4±0.2 0.14±0.02 4.6±0.4

> DR 24-month old rat

> RA <0.1 1.2±0.2 0.74±0.13*** 1.6±0.1¶

> LV <0.1 6.0±0.8¶¶ 0.24±0.01 3.5±0.1

> =====================================

> #P = 0.0449 vs. RA 24 month DR; ##P <0.0001 vs. RA 24 month IF;

*P < 0.0001 vs.

> LV 24 month DR; **P = 0.0003 vs. LV 24 month IF; ***P = 0.0058 vs.

RA 24 month IF;

> ¶P <0.0001 vs. RA 24 month IF; ¶¶P < 0.0001 vs. LV 24 month IF.

>

> -- Al Pater, alpater@...

>

> __________________________________________________

>

[Guest guest]

Hi folks:

Fig 1 in this study Al posted speaks for itself regarding the

benefits of CR or IF versus Ad lib. But, to me at least, the heat

shock protein 'thing' didn't mean much.

Fwiw, it now appears that HSPs are produced in response to stresses

and are beneficial in protecting against the stress. With aging HSPs

are created much less in response to a stress. The data in this

study show very clearly that the decline in production of HSPs is

much reduced in both regular CR and in IF. More so in IF than in

regular CR, despite higher caloric intake in the case of IF.

Rodney.

>

> Heat shock proteins are differentially regulated by the style of

CR, regular or

> intermittent feeding.

>

> Although the below pdf-available paper appeared to represent not

particularly

> practical results in the title nor abstract, the text informed.

The Fig. 1 data

> were mentioned only in the methods section of the text, but had

what were for me

> intriguing results. The Fig. 1 results other than the title are

eye-ball data,

> below. Intermittent feeding may require more consideration.

>

> Colotti C, Cavallini G, Vitale RL, Donati A, Maltinti M, Del Ry S,

Bergamini E,

> Giannessi D.

> Effects of aging and anti-aging caloric restrictions on carbonyl

and heat shock

> protein levels and expression.

> Biogerontology. 2005;6(6):397-406.

> PMID: 16518701

>

> http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?

cmd=Retrieve & db=pubmed & dopt=Abstract & list_uids=16518701 & query_hl=1 & ito

ol=pubmed_docsum

>

> ... Young (3-month-old) male Sprague Dawley rats were divided in

groups. A group was

> used to assess values at the younger age. Three groups were raised

on different

> nutritional regimens: rats in the first group were fed ad libitum a

standard Teklad

> diet (Harlan Italy) for life (AL); the second and the third groups

were on caloric

> restriction and fed 60% the amount of food consumed by AL rats

(DR), or were fed ad

> libitum every other day (intermittent feeding, IF) until the age of

24 months. Body

> weight, food consumption and survival are given (Figure 1). ...

>

> [Figure 1. (a) Body weight, ( Food consumption, © Survival of

Sprague Dawley

> rats fed ad libitum (AL), on caloric restriction diet (DR) and fed

ad libitum every

> other day (IF).

> ==========================================

> Feeding Body weight (g) Food consumption (g/day) Percent surviving

> ==========================================

> AL 560 19 61

> DR 380 12 72

> IF 420 17 85

> ==========================================

> 24-month values are provided.]

>

> Table 2. Heat shock protein levels in cardiac tissue of 3-month and

AL 24-month-old

> rat.

> =============================================================

> Protein content 3 months AL 24 months

> =============================================================

> Hsp27 (lg/mg of total proteins) (Mean±SEM)

> LV 0.72±0.35 <0.1

> RA 2.03±0.68 <0.1

> Hsp60 (lg/mg of total proteins) (Mean±SEM)

> LV 5.49±0.08* 3.57±0.19

> RA 3.71±0.36** 1.13±0.16

> Hsp72 (lg/mg of total proteins) (Mean±SEM)

> LV 0.55±0.28 0.21±0.06

> RA 0.99±0.18*** 0.35±0.07

> Hsc70 (lg/mg of total proteins) (Mean±SEM)

> LV 6.85±0.29**** 2.80±0.22

> RA 11.29±0.44***** 1.03±0.17

> =============================================================

> LV: Left Ventricle RA: Right Atrium.

> *P = 0.007; **P < 0.0001; ***P = 0.0018; ****P < 0.0001; *****P

< 0.0001 vs.

> corresponding AL 24 months.

>

> Table 3. Heat shock protein levels in cardiac tissue of 24-month-

old rats.

> =====================================

> Group Hsp27 (mean±SEM) (lg/mg) Hsp60 (mean±SEM) (lg/mg) Hsp72

(mean±SEM) (lg/mg)

> Hsc70 (mean±SEM) (lg/mg)

> =====================================

> AL 24-monthold rat

> RA <0.1 1.1±0.1 0.35±0.07# 1.0±0.2##

> LV <0.1 3.6±0.2* 0.21±0.06 2.8±0.2**

> IF 24-monthold rat

> RA <0.1 1.6±0.1 0.18±0.04 3.7±0.5

> LV <0.1 3.4±0.2 0.14±0.02 4.6±0.4

> DR 24-month old rat

> RA <0.1 1.2±0.2 0.74±0.13*** 1.6±0.1¶

> LV <0.1 6.0±0.8¶¶ 0.24±0.01 3.5±0.1

> =====================================

> #P = 0.0449 vs. RA 24 month DR; ##P <0.0001 vs. RA 24 month IF;

*P < 0.0001 vs.

> LV 24 month DR; **P = 0.0003 vs. LV 24 month IF; ***P = 0.0058 vs.

RA 24 month IF;

> ¶P <0.0001 vs. RA 24 month IF; ¶¶P < 0.0001 vs. LV 24 month IF.

>

> -- Al Pater, alpater@...

>

> __________________________________________________

>