Re: C.J.'s genetic variations results--Yasko panel

[Guest guest]

Hi, all.

I want to make a correction to my comment about the SUOX SNP below.

has written me that I have the interpretation backward:

" Hi Rich,

I just wanted to mention that I searched Dr. Amy's forum for info on

the SUOX ++ mutatation when I received my panel results showing

SUOX++.

It turns out that SUOX++ is normal. Here is a quote from Dr. Amy:

'The SUOX enzyme helps to detoxify sulfites that are generated due to

activity of the CBS gene. When we have increased CBS activity (CBS +)

then it can put additional strain on the SUOX enzyme.

....

In case of SUOX, I find that a - denotes situations where we are

having a potential issue with this SNP.

In virtually all cases we are finding a ++ status for the two SUOX

markers. In rare cases I have seen a single - for one of the two SUOX

SNPs and this single - for SUOX S370S appears to indicate a problem

with SUOX function.

Individuals who are CBS + and SUOX S370S- will have greater

difficulties with the use of sulfur donors.'

"

So, as I have now written CJ, her SUOX SNP will not exacerbate the

effect of her CBS SNP. Nevertheless, she still may have to limit

her intake of sulfur-containing foods and supplements just because

of the CBS SNP, and she still may need more molybdenum to support

the extra flow of sulfite through the SUOX enzyme. She still might

benefit from getting extra sulfate, and if she gets too much, it

will be filtered out by the kidneys and excreted in the urine. So

the interpretation of what to do doesn't change, qualitatively, but

the actual effect of her SUOX SNP is beneficial, rather than

deleterious.

Sorry about the error. I'm still learning about the interpretation

of these SNPs.

Rich

>

> Hi, all.

>

> I recently responded to C.J.'s request for comments on her

> methylation cycle genetic variations panel (Yasko panel). With

her

> permission, I am posting our message traffic here, so others can

> hopefully benefit from the information:

>

> Hi, CJ.

>

> Well, I'm finally getting to your genetic variations results.

Sorry

> for the delay. I'm pretty overloaded, as usual.

>

> There's a lot that I still don't know about the Yasko stuff, but I

> will share what I think I know. I would be interested to hear what

> you eventually receive from Dr. Yasko about your results.

>

> The first thing to do is to make sure you are not getting

> excitotoxins in your diet.

> This includes foods that contain MSG or related substances, or

> aspartame (Nutrasweet).

>

> Then the first SNPs to look at are the CBS SNPs. You have a CBS

> C699T (+/-). This SNP increases the activity of the cystathionine

> beta synthase enzyme, which converts homocysteine to cystathione

and

> thus moves sulfur substrates out of the methylation cycle and into

> the transsulfuration pathway. Having this speeded up can cause

> problems with your methylation cycle as well as your glutathione.

It

> can also make too much ammonia and sulfites, which are toxic, and

> too much taurine. So Dr. Yasko deals with this next. She

recommends

> limiting the amount of protein in the diet, and taking things like

> trimethylglycine (betaine), phosphatydylcholine (lecithin) or

> phosphatidylserine to speed up the alternate pathway from

> homocysteine to methionine. You should not take taurine, because

you

> are likely to already have an excess of it.

>

> Next, you have MTHFR C677T (+/+). This will slow the conversion of

> 5,10 methylene tetrahydrofolate to 5-methyl tetrahydrofolate.

> Because of this, you should benefit from taking folinic acid

(which

> is in Yasko's Intrinsic B12 supplement), and FolaPro, which is 5-

> methyl tetrahydrofolate. These are active forms of folate that

will

> make up for your inability to convert from one to another at

normal

> rates.

>

> Next, you have MTRR A66G (+/-) , MTRR H595Y (+/-), MTRR S175L (+/-

),

> and MTRR K350A (+/-). The first two will impair the recycling of

> methyl B12. The third may counter the first two somewhat, and I

> don't know about the fourth one. Based on what I have read so far,

I

> think you would benefit from taking methyl B12 or

hydroxocobalamin.

> Dr. Yasko favors hydroxocobalamin for someone with your

combination

> of SNPs, but I think the DAN! doctors would use methylcobalamin,

so

> I'm not sure which is the best. I would probably try

methylcobalamin

> first, because that's the final form that's actually needed by the

> methylation cycle. Sounds like you're already taking B12, but I

> don't know what form.

>

> Next, you have MAOA R207R (+/+). The monoamine oxidase A enzyme

> breaks down serotonin, and this SNP slows it down, so you are

likely

> to have higher levels of serotonin than normal. This might give

you

> mood swings, or aggressive behavior, or problems with gut

motility.

> Sounds like you don't have problems with mood swings (because the

> serotonin tends to oscillate when this enzyme is slowed down) or

> aggressive behavior. You should not take 5-HTP or tryptophan as

> supplements, because these will increase serotonin even more.

>

> Next, you have COMT V158M (+/+) and COMT H62H (+/+). Catechol-O-

> methyl transferase is the enzyme that breaks down dopamine,

> norepinephrine and epinephrine, and both these SNPs slow it down,

> increasing the levels of these substances. This can produce mood

> swings, also, because the level of dopamine will tend to

oscillate.

> You should not take tyrosine or phenylalanine as supplements,

> because these will increase the amounts of these substances even

> more.

>

> Next, you have ACE Del 16 (+/+). The angiotensin converting enzyme

> converts angiotensin I to angiotensin II. This SNP increases the

> activity of this enzyme, which will increase the level of

> angiotensin II and of aldosterone. This in turn causes more

> retention of sodium and more excretion of potassium into the

urine.

> The adrenals can become fatigued and produce the opposite effect,

> also. The combination of this SNP with your MAOA R207R (+/+) may

> produce low frustration thresholds and anxiety. Sounds like you

have

> figured out how to cope with these. This SNP is also correlated

with

> increased risk for heart disease.

>

> Next, you have NOS D298E (+/-). This SNP decreases the activity of

> the nitric oxide synthase enzyme. Combined with your CBS C699T (+/-

> ), this may give more difficulty in detoxing ammonia. You might

> consider taking one or two capsules of activated charcoal at

bedtime

> to bind ammonia in the gut and take it out.

>

> Next, you have SUOX S370S (+/+). This SNP lowers the activity of

> sulfite oxidase. Combined with your CBS C699T (+/-), this could

make

> you more vulnerable to sulfites and to foods and supplements that

> contain sulfur. It could also lower your sulfate level. It might

be

> beneficial to control your intake of sulfur-containing foods and

> supplements to a level you can tolerate. Taking supplemental

> molybdenum may help you to tolerate these better. You might also

> consider taking magnesium sulfate (Epsom salt) as a supplement, or

> adding it to your bathwater to raise your sulfate level.

>

> Next, you have VDR Bsm/Taq (+/+). This vitamin D receptor SNP

tends

> to decrease dopamine levels, which will help to counteract your

COMT

> polymorphisms.

>

> Next, you have VDR Fok (+/+). This may affect blood sugar

> regulation. It sounds as though you have that covered with diet

and

> chromium.

>

> You also have VDR Taq (+/+). I don't have any information on this

> one.

>

> So the sequence of treatments should start out with eliminating

> excitotoxins and cutting back on protein in the diet if it's high.

> You can start the charcoal, the molybdenum and the Epsom salt.

>

> Then take TMG, or phosphatidylcholine or phosphatidylserine.

>

> Then add RNA. This will help to make up for the folate metabolism

> problems early in the treatment.

>

> Then add methyl B12 (or maybe hydroxo B12), folinic acid and

> FolaPro.

>

> Then add DMG after the methylation cycle has gotten going well.

This

> will slow the alternate pathway from homocysteine to methionine,

> encouraging the main (B12-folate) pathway. It will also help with

> the folate metabolism.

>

> For detoxing, Dr. Yasko suggests CCK (cholecystokinin)/BioThyro,

low-

> dose EDTA, Beyond Clean in the bath, malic acid, horsetail grass,

> and her gut program.

>

> She recommends monitoring progress using the spot urine metals

test

> from Doctors Data. She encourages people to get these fairly

often.

>

> She also uses urine organic acids tests from Great Plains Lab or

> Genova Diagnostics, and urine amino acids tests from Doctor's

Data,

> to get an overall picture of what's going on with the methylation

> cycle.

>

> As you probably know, she recommends quite a number of

supplements,

> including her HHC general vitamin, and also Gaba to calm the

brain.

>

> I don't know what doses of these things should be used. Maybe you

> can get some guidance from Yasko's book Genetic Bypass, a copy of

> which should have come with your test kit. I also don't have a

good

> feel for how long you should try one supplement before adding

> another. The impression I get from reading Dr. Yasko's materials

is

> that it can take some months to do the treatments, so it's

probably

> a good idea to be patient with them and don't try to compress the

> treatment into too short a time.

>

> I think that's about all I can say at this point. As I said, I'm

> still learning about this, especially as applied to CFS, but maybe

> these comments will be of some help.

>

> Rich

>