Re: DNA adducts results - Rich

[Guest guest]

Have you looked into nebulizing glutathione? From what I've read a researched

it seems to be the best way to get it into your system. I get mine from Key

Labs.

lowcreoch <sllc53@...> wrote: Hi Rich and All,

I've got the results of my DNA adducts

test from Biolab.This test measures exogenous and endogenous toxins

stuck to your DNA.Having these adducts is not good news, it means they

block the genes and stops them from working properly, or if it is

stuck next to a gene, that gene may be activated. DNA adducts are

precancerous lesions.Usually a zinc dependent enzyme corrects these

faults in the DNA. Dr Myhill says these adducts are correctable so

though it isn't very jolly to get these results I am glad that I know

and can do something about it.

My results showed that zinc (normally DNA associated) was 22ng/ml

(ref 21-74)

I have " poor zinc association, DNA is vulnerable to mutation and toxic

insult especially toxic metals. " So I am not good at correcting these

lesions.

I have 2 organic chemical adducts,

1. Malondialdehyde 29ng/ml (normal zero)

2. Diolein 12ng/ml (normal zero)

" Malondialdehyde is an endogenous product of peroxidation of lipids

and is also formed during the production of prostoglandins. This

adduct significantly contributes to cancers that are linked to

lifestyle and nutritional factors.Both of these are adducts were

spread over several chromosones. " They are both lipid peroxidation

products.They occur as a result of poor antioxidant status and/or

because of high levels of free radicals

I have googled malondialdehyde and it certainly does have very

definite associations with cancer risk, alzheimers etc which is a bit

scary.

Well, in my ATP profile (see post 94837) I came up with a " clinically

significant deficiency of SODase " and " very significant increase in

cell degradation " and also " rather poor levels of CoQ " plus low

magnesium and low ATP and marked B3 deficiency - so these all point

to poor antioxidant status.

Yet my RBC GSH was low normal.It seems very surprising to me (in my

ignorance!) that I don't have a very much lower GSH level if I have

such poor antioxidant status and high levels of cell degradation etc.

plus these DNA adducts.

As prescribed by Dr Myhill I am already taking copper, manganese and

zinc to correct the SODase problem and sublingual methylcobalamin

1mg/day to help with the nitric oxide and peroxynitrite.

I take 400iu vitamin E and 2g of Vit C, selenium 200micrograms, Vit A

and betacarotene in my multivit/min which is a good high potency one.

I am also taking magnesium, B3, acetly-L- carnitine, Co Q, chromium,

kelp, green tea extract, fish oil, starflower oil.

I eat a good diet, organic if possible, lots of fruit and veg, no

drink, no smoking, I've been taking supplements for decades now yet I

am coming up with all these high levels of free radicals and poor

antioxidant status. Why? Is it all down to glutathione or is there

more to it?

Rich, if you have a minute could you please give me some advice?

Dr Myhill suggested going up to bowel tolerance for Vit C for its

antioxidant properties and also because it helps to recycle endogenous

SODase and glutathione peroxidase.

The question is I am not sure about taking this high Vit C because

you have said it has a negative impact on glutathione, so as I am low

normal for GSH, I have recently reduced my intake from 4g to 2 g. So

what sort of dose do you think I should take, high or low?

It seems, as you said the other day, that I obviously need to try to

increase my GSH, what method would you suggest, taking lipo

glutathione, or sublingual glutathione or taking whey, or going down

the DAN route or a combination thereof?

Can it be that I am not using my GSH properly, is there any other

tests for GSH and related enzymes etc that you think would be worth

taking to try to explain what is going on?

With many thanks for your help, looking forward to hearing the radio

programmes,

Sheila

This list is intended for patients to share personal experiences with each

other, not to give medical advice. If you are interested in any treatment

discussed here, please consult your doctor.

[Guest guest]

Hi Edy, thanks for that, I had completely forgotten about nebulising

GSH, memory like a sieve. I have a nebuliser so that might be the way

to go. Do you know if it is better than lipo glutathione?

BW,

Sheila

Hi Rich and All,

> I've got the results of my DNA adducts

> test from Biolab.This test measures exogenous and endogenous toxins

> stuck to your DNA.Having these adducts is not good news, it means they

> block the genes and stops them from working properly, or if it is

> stuck next to a gene, that gene may be activated. DNA adducts are

> precancerous lesions.Usually a zinc dependent enzyme corrects these

> faults in the DNA. Dr Myhill says these adducts are correctable so

> though it isn't very jolly to get these results I am glad that I know

> and can do something about it.

>

> My results showed that zinc (normally DNA associated) was 22ng/ml

> (ref 21-74)

> I have " poor zinc association, DNA is vulnerable to mutation and toxic

> insult especially toxic metals. " So I am not good at correcting these

> lesions.

>

> I have 2 organic chemical adducts,

> 1. Malondialdehyde 29ng/ml (normal zero)

> 2. Diolein 12ng/ml (normal zero)

> " Malondialdehyde is an endogenous product of peroxidation of lipids

> and is also formed during the production of prostoglandins. This

> adduct significantly contributes to cancers that are linked to

> lifestyle and nutritional factors.Both of these are adducts were

> spread over several chromosones. " They are both lipid peroxidation

> products.They occur as a result of poor antioxidant status and/or

> because of high levels of free radicals

>

> I have googled malondialdehyde and it certainly does have very

> definite associations with cancer risk, alzheimers etc which is a bit

> scary.

>

> Well, in my ATP profile (see post 94837) I came up with a " clinically

> significant deficiency of SODase " and " very significant increase in

> cell degradation " and also " rather poor levels of CoQ " plus low

> magnesium and low ATP and marked B3 deficiency - so these all point

> to poor antioxidant status.

>

> Yet my RBC GSH was low normal.It seems very surprising to me (in my

> ignorance!) that I don't have a very much lower GSH level if I have

> such poor antioxidant status and high levels of cell degradation etc.

> plus these DNA adducts.

>

> As prescribed by Dr Myhill I am already taking copper, manganese and

> zinc to correct the SODase problem and sublingual methylcobalamin

> 1mg/day to help with the nitric oxide and peroxynitrite.

> I take 400iu vitamin E and 2g of Vit C, selenium 200micrograms, Vit A

> and betacarotene in my multivit/min which is a good high potency one.

> I am also taking magnesium, B3, acetly-L- carnitine, Co Q, chromium,

> kelp, green tea extract, fish oil, starflower oil.

>

> I eat a good diet, organic if possible, lots of fruit and veg, no

> drink, no smoking, I've been taking supplements for decades now yet I

> am coming up with all these high levels of free radicals and poor

> antioxidant status. Why? Is it all down to glutathione or is there

> more to it?

>

> Rich, if you have a minute could you please give me some advice?

>

> Dr Myhill suggested going up to bowel tolerance for Vit C for its

> antioxidant properties and also because it helps to recycle endogenous

> SODase and glutathione peroxidase.

>

> The question is I am not sure about taking this high Vit C because

> you have said it has a negative impact on glutathione, so as I am low

> normal for GSH, I have recently reduced my intake from 4g to 2 g. So

> what sort of dose do you think I should take, high or low?

>

> It seems, as you said the other day, that I obviously need to try to

> increase my GSH, what method would you suggest, taking lipo

> glutathione, or sublingual glutathione or taking whey, or going down

> the DAN route or a combination thereof?

>

> Can it be that I am not using my GSH properly, is there any other

> tests for GSH and related enzymes etc that you think would be worth

> taking to try to explain what is going on?

>

> With many thanks for your help, looking forward to hearing the radio

> programmes,

>

> Sheila

>

>

>

>

>

>

> This list is intended for patients to share personal experiences

with each other, not to give medical advice. If you are interested in

any treatment discussed here, please consult your doctor.

>

>

>

>

[Guest guest]

>

>

> Hi Edy, thanks for that, I had completely forgotten about nebulising

> GSH, memory like a sieve. I have a nebuliser so that might be the way

> to go. Do you know if it is better than lipo glutathione?

> BW,

> Sheila

HI Sheila

Thanks for posting your results but sorry to hear of the problems with

those chemicals and yet as you say your rbc glutathione was low normal

like mine.

Just wanted to mention that I have been nebulsing magnesium since

November and now my level has moved up to low normal from low so it

does seem to be a good route to get much needed " stuff " into our

bodies although in my case it is slow progress.

I too would be very interested about nebulising glutathione to see if

raising it would help my body deal with the high DNA/RNA problem which

Dr H thinks is probably viral.

Best Wishes

Pam

PS Is there a link to all this and underfunctioning adrenals/thyroid -

I rather guess there is?

[Guest guest]

Thanks Pam amd Edy for your replies.

Pam thanks for mentioning your Mg levels and nebulising, I will bear

that in mind too. Have you had any problems with nebulising it ie

allergic/asthmatic reactions? Or has it been pretty plain sailing?

Also thanks for posting about your B12 levels and how the sublingual

methyl B12 worked for you as well as the injections, that is heartening.

Dr M wants me to get B12 injections but our GP practice will not allow

it, so it is a none starter, so I have started the sublingual M B12

and it is reassuring to know it works.

Good luck trying to sort out the translocator/viral problem.

BW,

Sheila

> >

> >

> > Hi Edy, thanks for that, I had completely forgotten about nebulising

> > GSH, memory like a sieve. I have a nebuliser so that might be the way

> > to go. Do you know if it is better than lipo glutathione?

> > BW,

> > Sheila

>

> HI Sheila

>

> Thanks for posting your results but sorry to hear of the problems with

> those chemicals and yet as you say your rbc glutathione was low normal

> like mine.

>

> Just wanted to mention that I have been nebulsing magnesium since

> November and now my level has moved up to low normal from low so it

> does seem to be a good route to get much needed " stuff " into our

> bodies although in my case it is slow progress.

>

> I too would be very interested about nebulising glutathione to see if

> raising it would help my body deal with the high DNA/RNA problem which

> Dr H thinks is probably viral.

>

> Best Wishes

>

> Pam

>

> PS Is there a link to all this and underfunctioning adrenals/thyroid -

> I rather guess there is?

>

[Guest guest]

Hi Sheila,

Thanks for posting your results. I hope you can sort out these

adducts. I have DNA adducts for lindane and nickel. Dr M told me

aslo that zinc will help. She also suggested FIR saunas.

I've ben taking powdered green barley grass for a while and have

just started juicing wheatgrass. I came upon some research on these

grasses recently indicating that they help repair DNA and help raise

SODase levels. Green barley also helps increase the number of red

blood cells, hence more oxygenation and ATP.

Are you taking any antiinfection supplements? Infections can give

high amounts of free radicals etc.

Good luck,

Carol

>

> Hi Rich and All,

> I've got the results of my DNA adducts

> test from Biolab.This test measures exogenous and endogenous toxins

> stuck to your DNA.Having these adducts is not good news, it means

they

> block the genes and stops them from working properly, or if it is

> stuck next to a gene, that gene may be activated. DNA adducts are

> precancerous lesions.Usually a zinc dependent enzyme corrects these

> faults in the DNA. Dr Myhill says these adducts are correctable so

> though it isn't very jolly to get these results I am glad that I

know

> and can do something about it.

>

> My results showed that zinc (normally DNA associated) was

22ng/ml

> (ref 21-74)

> I have " poor zinc association, DNA is vulnerable to mutation and

toxic

> insult especially toxic metals. " So I am not good at correcting

these

> lesions.

>

> I have 2 organic chemical adducts,

> 1. Malondialdehyde 29ng/ml (normal zero)

> 2. Diolein 12ng/ml (normal zero)

> " Malondialdehyde is an endogenous product of peroxidation of

lipids

> and is also formed during the production of prostoglandins. This

> adduct significantly contributes to cancers that are linked to

> lifestyle and nutritional factors.Both of these are adducts were

> spread over several chromosones. " They are both lipid peroxidation

> products.They occur as a result of poor antioxidant status and/or

> because of high levels of free radicals

>

> I have googled malondialdehyde and it certainly does have very

> definite associations with cancer risk, alzheimers etc which is a

bit

> scary.

>

> Well, in my ATP profile (see post 94837) I came up with a "

clinically

> significant deficiency of SODase " and " very significant increase in

> cell degradation " and also " rather poor levels of CoQ " plus low

> magnesium and low ATP and marked B3 deficiency - so these all

point

> to poor antioxidant status.

>

> Yet my RBC GSH was low normal.It seems very surprising to me (in my

> ignorance!) that I don't have a very much lower GSH level if I have

> such poor antioxidant status and high levels of cell degradation

etc.

> plus these DNA adducts.

>

> As prescribed by Dr Myhill I am already taking copper, manganese

and

> zinc to correct the SODase problem and sublingual methylcobalamin

> 1mg/day to help with the nitric oxide and peroxynitrite.

> I take 400iu vitamin E and 2g of Vit C, selenium 200micrograms,

Vit A

> and betacarotene in my multivit/min which is a good high potency

one.

> I am also taking magnesium, B3, acetly-L- carnitine, Co Q,

chromium,

> kelp, green tea extract, fish oil, starflower oil.

>

> I eat a good diet, organic if possible, lots of fruit and veg, no

> drink, no smoking, I've been taking supplements for decades now

yet I

> am coming up with all these high levels of free radicals and poor

> antioxidant status. Why? Is it all down to glutathione or is there

> more to it?

>

> Rich, if you have a minute could you please give me some advice?

>

> Dr Myhill suggested going up to bowel tolerance for Vit C for its

> antioxidant properties and also because it helps to recycle

endogenous

> SODase and glutathione peroxidase.

>

> The question is I am not sure about taking this high Vit C because

> you have said it has a negative impact on glutathione, so as I am

low

> normal for GSH, I have recently reduced my intake from 4g to 2 g.

So

> what sort of dose do you think I should take, high or low?

>

> It seems, as you said the other day, that I obviously need to try

to

> increase my GSH, what method would you suggest, taking lipo

> glutathione, or sublingual glutathione or taking whey, or going

down

> the DAN route or a combination thereof?

>

> Can it be that I am not using my GSH properly, is there any other

> tests for GSH and related enzymes etc that you think would be

worth

> taking to try to explain what is going on?

>

> With many thanks for your help, looking forward to hearing the

radio

> programmes,

>

> Sheila

>

[Guest guest]

Hi Carol,

Thanks for telling me about the green barley grass, that sounds just

what I need! I wonder if people with allergies to these grains can

tolerate the sprouts? May I ask which brand you are taking?

Yes I have borreliosis and I am sure that is contributing to all this.

I am taking samento and colloidal silver. If I take to much of either

of these I go downhill as I can't take the kill off possibly because

that puts a further load on the antioxidant system, so I have to go

slowly but I hope that as I sort out all of this I will be able to

take more samento etc to kill off the beasties and anything else that

might be there.

I am not sure if FIR sauna is right for the adducts I have, Dr M

mentioned it in my ATP results when she was thinking I might have

chemiclal or metal adducts but in this report she hasn't suggested it

as a form of treatment so I guess I will have to phone up and find

out. I am not a patient of Dr M as her books are full, merely getting

the tests done through her and getting the report with the test, which

isn't ideal as you can't question her and also she only has a snapshot

of my health history. But I am grateful for her help none the less,

these tests have definitely been worth the money.

Good luck to you toogetting rid of those adducts, keep us posted as to

how you get on.

BW,

Sheila

> >

> > Hi Rich and All,

> > I've got the results of my DNA adducts

> > test from Biolab.This test measures exogenous and endogenous toxins

> > stuck to your DNA.Having these adducts is not good news, it means

> they

> > block the genes and stops them from working properly, or if it is

> > stuck next to a gene, that gene may be activated. DNA adducts are

> > precancerous lesions.Usually a zinc dependent enzyme corrects these

> > faults in the DNA. Dr Myhill says these adducts are correctable so

> > though it isn't very jolly to get these results I am glad that I

> know

> > and can do something about it.

> >

> > My results showed that zinc (normally DNA associated) was

> 22ng/ml

> > (ref 21-74)

> > I have " poor zinc association, DNA is vulnerable to mutation and

> toxic

> > insult especially toxic metals. " So I am not good at correcting

> these

> > lesions.

> >

> > I have 2 organic chemical adducts,

> > 1. Malondialdehyde 29ng/ml (normal zero)

> > 2. Diolein 12ng/ml (normal zero)

> > " Malondialdehyde is an endogenous product of peroxidation of

> lipids

> > and is also formed during the production of prostoglandins. This

> > adduct significantly contributes to cancers that are linked to

> > lifestyle and nutritional factors.Both of these are adducts were

> > spread over several chromosones. " They are both lipid peroxidation

> > products.They occur as a result of poor antioxidant status and/or

> > because of high levels of free radicals

> >

> > I have googled malondialdehyde and it certainly does have very

> > definite associations with cancer risk, alzheimers etc which is a

> bit

> > scary.

> >

> > Well, in my ATP profile (see post 94837) I came up with a "

> clinically

> > significant deficiency of SODase " and " very significant increase in

> > cell degradation " and also " rather poor levels of CoQ " plus low

> > magnesium and low ATP and marked B3 deficiency - so these all

> point

> > to poor antioxidant status.

> >

> > Yet my RBC GSH was low normal.It seems very surprising to me (in my

> > ignorance!) that I don't have a very much lower GSH level if I have

> > such poor antioxidant status and high levels of cell degradation

> etc.

> > plus these DNA adducts.

> >

> > As prescribed by Dr Myhill I am already taking copper, manganese

> and

> > zinc to correct the SODase problem and sublingual methylcobalamin

> > 1mg/day to help with the nitric oxide and peroxynitrite.

> > I take 400iu vitamin E and 2g of Vit C, selenium 200micrograms,

> Vit A

> > and betacarotene in my multivit/min which is a good high potency

> one.

> > I am also taking magnesium, B3, acetly-L- carnitine, Co Q,

> chromium,

> > kelp, green tea extract, fish oil, starflower oil.

> >

> > I eat a good diet, organic if possible, lots of fruit and veg, no

> > drink, no smoking, I've been taking supplements for decades now

> yet I

> > am coming up with all these high levels of free radicals and poor

> > antioxidant status. Why? Is it all down to glutathione or is there

> > more to it?

> >

> > Rich, if you have a minute could you please give me some advice?

> >

> > Dr Myhill suggested going up to bowel tolerance for Vit C for its

> > antioxidant properties and also because it helps to recycle

> endogenous

> > SODase and glutathione peroxidase.

> >

> > The question is I am not sure about taking this high Vit C because

> > you have said it has a negative impact on glutathione, so as I am

> low

> > normal for GSH, I have recently reduced my intake from 4g to 2 g.

> So

> > what sort of dose do you think I should take, high or low?

> >

> > It seems, as you said the other day, that I obviously need to try

> to

> > increase my GSH, what method would you suggest, taking lipo

> > glutathione, or sublingual glutathione or taking whey, or going

> down

> > the DAN route or a combination thereof?

> >

> > Can it be that I am not using my GSH properly, is there any other

> > tests for GSH and related enzymes etc that you think would be

> worth

> > taking to try to explain what is going on?

> >

> > With many thanks for your help, looking forward to hearing the

> radio

> > programmes,

> >

> > Sheila

> >

>

[Guest guest]

Hi Sheila,

I have multiple food intolerances including gluten and wheat and I

tolerate the grasses very well. There is no gluten in the grass. I

get my green barley from Safe Remedies

http://www.saferemedies.net/ - I've been on it for over a couple of

years. I buy it in kilo sized pots and they last for many weeks. I

find it energizing and it doesn't give me strong herxlike detoxes,

but it can for some. I initially started it after getting very

sticky blood following half of my amalgams being removed - and my

blood unstuck again after taking it. I also take Prime Directive

from Safe Remedies - which is a superfood plus probiotic actually

designed for MEs and that can give strong herx like detoxes, and

many people have to start on one eighth of the amount and build up

very slowly which also makes it cheaper.

The wheatgrass I get from my local organic veg delivery people -

its made from spelt wheat rather than normal wheat. The suppliers

of the grass are http://www.aconbury.co.uk/ and they can give you

local suppliers in your area. I needed to get a proper veg juicer

though, centrifugal ones won't be good enough. I get detox effects

from this.

Cheers,

Carol

> > >

> > > Hi Rich and All,

> > > I've got the results of my DNA

adducts

> > > test from Biolab.This test measures exogenous and endogenous

toxins

> > > stuck to your DNA.Having these adducts is not good news, it

means

> > they

> > > block the genes and stops them from working properly, or if it

is

> > > stuck next to a gene, that gene may be activated. DNA adducts

are

> > > precancerous lesions.Usually a zinc dependent enzyme corrects

these

> > > faults in the DNA. Dr Myhill says these adducts are

correctable so

> > > though it isn't very jolly to get these results I am glad that

I

> > know

> > > and can do something about it.

> > >

> > > My results showed that zinc (normally DNA associated) was

> > 22ng/ml

> > > (ref 21-74)

> > > I have " poor zinc association, DNA is vulnerable to mutation

and

> > toxic

> > > insult especially toxic metals. " So I am not good at

correcting

> > these

> > > lesions.

> > >

> > > I have 2 organic chemical adducts,

> > > 1. Malondialdehyde 29ng/ml (normal zero)

> > > 2. Diolein 12ng/ml (normal zero)

> > > " Malondialdehyde is an endogenous product of peroxidation of

> > lipids

> > > and is also formed during the production of prostoglandins.

This

> > > adduct significantly contributes to cancers that are linked to

> > > lifestyle and nutritional factors.Both of these are adducts

were

> > > spread over several chromosones. " They are both lipid

peroxidation

> > > products.They occur as a result of poor antioxidant status

and/or

> > > because of high levels of free radicals

> > >

> > > I have googled malondialdehyde and it certainly does have very

> > > definite associations with cancer risk, alzheimers etc which

is a

> > bit

> > > scary.

> > >

> > > Well, in my ATP profile (see post 94837) I came up with a "

> > clinically

> > > significant deficiency of SODase " and " very significant

increase in

> > > cell degradation " and also " rather poor levels of CoQ " plus

low

> > > magnesium and low ATP and marked B3 deficiency - so these all

> > point

> > > to poor antioxidant status.

> > >

> > > Yet my RBC GSH was low normal.It seems very surprising to me

(in my

> > > ignorance!) that I don't have a very much lower GSH level if I

have

> > > such poor antioxidant status and high levels of cell

degradation

> > etc.

> > > plus these DNA adducts.

> > >

> > > As prescribed by Dr Myhill I am already taking copper,

manganese

> > and

> > > zinc to correct the SODase problem and sublingual

methylcobalamin

> > > 1mg/day to help with the nitric oxide and peroxynitrite.

> > > I take 400iu vitamin E and 2g of Vit C, selenium

200micrograms,

> > Vit A

> > > and betacarotene in my multivit/min which is a good high

potency

> > one.

> > > I am also taking magnesium, B3, acetly-L- carnitine, Co Q,

> > chromium,

> > > kelp, green tea extract, fish oil, starflower oil.

> > >

> > > I eat a good diet, organic if possible, lots of fruit and veg,

no

> > > drink, no smoking, I've been taking supplements for decades

now

> > yet I

> > > am coming up with all these high levels of free radicals and

poor

> > > antioxidant status. Why? Is it all down to glutathione or is

there

> > > more to it?

> > >

> > > Rich, if you have a minute could you please give me some

advice?

> > >

> > > Dr Myhill suggested going up to bowel tolerance for Vit C for

its

> > > antioxidant properties and also because it helps to recycle

> > endogenous

> > > SODase and glutathione peroxidase.

> > >

> > > The question is I am not sure about taking this high Vit C

because

> > > you have said it has a negative impact on glutathione, so as I

am

> > low

> > > normal for GSH, I have recently reduced my intake from 4g to 2

g.

> > So

> > > what sort of dose do you think I should take, high or low?

> > >

> > > It seems, as you said the other day, that I obviously need to

try

> > to

> > > increase my GSH, what method would you suggest, taking lipo

> > > glutathione, or sublingual glutathione or taking whey, or

going

> > down

> > > the DAN route or a combination thereof?

> > >

> > > Can it be that I am not using my GSH properly, is there any

other

> > > tests for GSH and related enzymes etc that you think would be

> > worth

> > > taking to try to explain what is going on?

> > >

> > > With many thanks for your help, looking forward to hearing the

> > radio

> > > programmes,

> > >

> > > Sheila

> > >

> >

>

[Guest guest]

Hi, Sheila.

I, too, appreciate your posting your results.

In regard to the bowel-tolerance dosage of vitamin C, I do think

that could be beneficial for you. I have only recently come to the

realization that with very high dosage of vitamin C, the oxidation-

reduction reaction couple between glutathione and vitamin C can

actually be reversed, so that vitamin C will regenerate glutathione,

rather than loading it down and diminishing it. So low dosages of

vitamin C do not load glutathione, and neither do very high doses

(which actually help to regenerate it). Intermediate doses,

however, will tend to load glutathione, as Dr. Cheney originally

said.

Building up your other antioxidants with the supplements you are

taking should also be helpful, since the antioxidants work together,

and it's important to have enough of all of them in the basic

antioxidant network, which includes glutathione, vitamins E and C,

alpha lipoic acid, and coenzyme Q-10.

I do suspect that there may be genetic variations in your

glutathione transferase and glutathione peroxidase enzymes, from

what you've reported. I don't know if BioLab characterizes them.

The Genovations DetoxiGenomic Profile does characterize the

glutathione transferases, but not the glutathione peroxidases. The

Genova Comprehensive Detoxification profile measures the activity of

the glutathione peroxidase. I think Biolab may offer a test of the

activity of the glutathione transferases. If the activities are

low, that suggests genetic variations. Low activity in glutathione

peroxidase is most often due to low selenium, because that is a

cofactor for some of these enzymes.

Glutathione is able to react without the help of these enzymes to

some degree, so there is value in building it up even if the enzymes

have problems. Lipoceutical glutathione seems like a promising way

to do that.

I don't know whether it's possible to have a block in the

methylation cycle and to still have a normal level of glutathione,

if the enzymes that use it have genetic variations, but I suspect

not.

Perhaps trying to get a better idea about how well these supporting

enzymes are working would be worthwhile. I read in one of Dr.

's old newletters that he had found a few patients whose

glutathione peroxidase was missing one of its four tetramers (a

large part of the protein molecule), but I don't know if he offers

such a test routinely or whether it was only a small research

project that he did.

Rich

>

> Hi Rich and All,

> I've got the results of my DNA adducts

> test from Biolab.This test measures exogenous and endogenous toxins

> stuck to your DNA.Having these adducts is not good news, it means

they

> block the genes and stops them from working properly, or if it is

> stuck next to a gene, that gene may be activated. DNA adducts are

> precancerous lesions.Usually a zinc dependent enzyme corrects these

> faults in the DNA. Dr Myhill says these adducts are correctable so

> though it isn't very jolly to get these results I am glad that I

know

> and can do something about it.

>

> My results showed that zinc (normally DNA associated) was

22ng/ml

> (ref 21-74)

> I have " poor zinc association, DNA is vulnerable to mutation and

toxic

> insult especially toxic metals. " So I am not good at correcting

these

> lesions.

>

> I have 2 organic chemical adducts,

> 1. Malondialdehyde 29ng/ml (normal zero)

> 2. Diolein 12ng/ml (normal zero)

> " Malondialdehyde is an endogenous product of peroxidation of

lipids

> and is also formed during the production of prostoglandins. This

> adduct significantly contributes to cancers that are linked to

> lifestyle and nutritional factors.Both of these are adducts were

> spread over several chromosones. " They are both lipid peroxidation

> products.They occur as a result of poor antioxidant status and/or

> because of high levels of free radicals

>

> I have googled malondialdehyde and it certainly does have very

> definite associations with cancer risk, alzheimers etc which is a

bit

> scary.

>

> Well, in my ATP profile (see post 94837) I came up with a "

clinically

> significant deficiency of SODase " and " very significant increase in

> cell degradation " and also " rather poor levels of CoQ " plus low

> magnesium and low ATP and marked B3 deficiency - so these all

point

> to poor antioxidant status.

>

> Yet my RBC GSH was low normal.It seems very surprising to me (in my

> ignorance!) that I don't have a very much lower GSH level if I have

> such poor antioxidant status and high levels of cell degradation

etc.

> plus these DNA adducts.

>

> As prescribed by Dr Myhill I am already taking copper, manganese

and

> zinc to correct the SODase problem and sublingual methylcobalamin

> 1mg/day to help with the nitric oxide and peroxynitrite.

> I take 400iu vitamin E and 2g of Vit C, selenium 200micrograms,

Vit A

> and betacarotene in my multivit/min which is a good high potency

one.

> I am also taking magnesium, B3, acetly-L- carnitine, Co Q,

chromium,

> kelp, green tea extract, fish oil, starflower oil.

>

> I eat a good diet, organic if possible, lots of fruit and veg, no

> drink, no smoking, I've been taking supplements for decades now

yet I

> am coming up with all these high levels of free radicals and poor

> antioxidant status. Why? Is it all down to glutathione or is there

> more to it?

>

> Rich, if you have a minute could you please give me some advice?

>

> Dr Myhill suggested going up to bowel tolerance for Vit C for its

> antioxidant properties and also because it helps to recycle

endogenous

> SODase and glutathione peroxidase.

>

> The question is I am not sure about taking this high Vit C because

> you have said it has a negative impact on glutathione, so as I am

low

> normal for GSH, I have recently reduced my intake from 4g to 2 g.

So

> what sort of dose do you think I should take, high or low?

>

> It seems, as you said the other day, that I obviously need to try

to

> increase my GSH, what method would you suggest, taking lipo

> glutathione, or sublingual glutathione or taking whey, or going

down

> the DAN route or a combination thereof?

>

> Can it be that I am not using my GSH properly, is there any other

> tests for GSH and related enzymes etc that you think would be

worth

> taking to try to explain what is going on?

>

> With many thanks for your help, looking forward to hearing the

radio

> programmes,

>

> Sheila

>

[Guest guest]

Hi Carol,

Very many thanks for this info. I've just been reading up on barley

grass and it does sound very appropriate.I'm going to order some, it

sounds worth a go.

It is funny I grow sunflower seeds sprouts in the summer months when I

can get my energy together to do so and I just love them plus all raw

greens, my husband says I am a bit like a rabbit! So perhaps my body

is telling me something.

I'll let you know how I get on with it.

BW,

Sheila

> > > >

> > > > Hi Rich and All,

> > > > I've got the results of my DNA

> adducts

> > > > test from Biolab.This test measures exogenous and endogenous

> toxins

> > > > stuck to your DNA.Having these adducts is not good news, it

> means

> > > they

> > > > block the genes and stops them from working properly, or if it

> is

> > > > stuck next to a gene, that gene may be activated. DNA adducts

> are

> > > > precancerous lesions.Usually a zinc dependent enzyme corrects

> these

> > > > faults in the DNA. Dr Myhill says these adducts are

> correctable so

> > > > though it isn't very jolly to get these results I am glad that

> I

> > > know

> > > > and can do something about it.

> > > >

> > > > My results showed that zinc (normally DNA associated) was

> > > 22ng/ml

> > > > (ref 21-74)

> > > > I have " poor zinc association, DNA is vulnerable to mutation

> and

> > > toxic

> > > > insult especially toxic metals. " So I am not good at

> correcting

> > > these

> > > > lesions.

> > > >

> > > > I have 2 organic chemical adducts,

> > > > 1. Malondialdehyde 29ng/ml (normal zero)

> > > > 2. Diolein 12ng/ml (normal zero)

> > > > " Malondialdehyde is an endogenous product of peroxidation of

> > > lipids

> > > > and is also formed during the production of prostoglandins.

> This

> > > > adduct significantly contributes to cancers that are linked to

> > > > lifestyle and nutritional factors.Both of these are adducts

> were

> > > > spread over several chromosones. " They are both lipid

> peroxidation

> > > > products.They occur as a result of poor antioxidant status

> and/or

> > > > because of high levels of free radicals

> > > >

> > > > I have googled malondialdehyde and it certainly does have very

> > > > definite associations with cancer risk, alzheimers etc which

> is a

> > > bit

> > > > scary.

> > > >

> > > > Well, in my ATP profile (see post 94837) I came up with a "

> > > clinically

> > > > significant deficiency of SODase " and " very significant

> increase in

> > > > cell degradation " and also " rather poor levels of CoQ " plus

> low

> > > > magnesium and low ATP and marked B3 deficiency - so these all

> > > point

> > > > to poor antioxidant status.

> > > >

> > > > Yet my RBC GSH was low normal.It seems very surprising to me

> (in my

> > > > ignorance!) that I don't have a very much lower GSH level if I

> have

> > > > such poor antioxidant status and high levels of cell

> degradation

> > > etc.

> > > > plus these DNA adducts.

> > > >

> > > > As prescribed by Dr Myhill I am already taking copper,

> manganese

> > > and

> > > > zinc to correct the SODase problem and sublingual

> methylcobalamin

> > > > 1mg/day to help with the nitric oxide and peroxynitrite.

> > > > I take 400iu vitamin E and 2g of Vit C, selenium

> 200micrograms,

> > > Vit A

> > > > and betacarotene in my multivit/min which is a good high

> potency

> > > one.

> > > > I am also taking magnesium, B3, acetly-L- carnitine, Co Q,

> > > chromium,

> > > > kelp, green tea extract, fish oil, starflower oil.

> > > >

> > > > I eat a good diet, organic if possible, lots of fruit and veg,

> no

> > > > drink, no smoking, I've been taking supplements for decades

> now

> > > yet I

> > > > am coming up with all these high levels of free radicals and

> poor

> > > > antioxidant status. Why? Is it all down to glutathione or is

> there

> > > > more to it?

> > > >

> > > > Rich, if you have a minute could you please give me some

> advice?

> > > >

> > > > Dr Myhill suggested going up to bowel tolerance for Vit C for

> its

> > > > antioxidant properties and also because it helps to recycle

> > > endogenous

> > > > SODase and glutathione peroxidase.

> > > >

> > > > The question is I am not sure about taking this high Vit C

> because

> > > > you have said it has a negative impact on glutathione, so as I

> am

> > > low

> > > > normal for GSH, I have recently reduced my intake from 4g to 2

> g.

> > > So

> > > > what sort of dose do you think I should take, high or low?

> > > >

> > > > It seems, as you said the other day, that I obviously need to

> try

> > > to

> > > > increase my GSH, what method would you suggest, taking lipo

> > > > glutathione, or sublingual glutathione or taking whey, or

> going

> > > down

> > > > the DAN route or a combination thereof?

> > > >

> > > > Can it be that I am not using my GSH properly, is there any

> other

> > > > tests for GSH and related enzymes etc that you think would be

> > > worth

> > > > taking to try to explain what is going on?

> > > >

> > > > With many thanks for your help, looking forward to hearing the

> > > radio

> > > > programmes,

> > > >

> > > > Sheila

> > > >

> > >

> >

>

[Guest guest]

>

> Did you use an oral or a nasal nebulizer?

> Gail

>

Hi Gail

I presume one would call it an oral nebuliser, I just breathe it in

through my mouth but the mouth piece covers my nose as well!

Pam

[Guest guest]

Hi Rich,

Very many thanks for your reply. I am really grateful to get your

insights into all this and for your time.

May I ask you a quick couple of questions relating to your reply?

1. In the past you recommended a book on antioxidants. I am right in

remembering it was " The Antioxidant Miracle " by Lester Packer?

2. I will need to start taking ALA, that is the only one you mention I

am not on, and from what you say they all work synergistically.

I have been put off taking it in the past after reading how it

mobilises mercury and also the DAN book says they found some autistic

children developed " severe yeast infections " from taking it.

So is there any way I can avoid these pitfalls?

Also which form of ALA would you recommend, the R lipoic form or the

racemic form or would either do?

3. Yes you are right, I have just looked up the Biolab site and I can

get the glutathione peroxidase tested.

Here is what they say about their glutathione transferase test:-

Glutathione-S-transferase (G-S-T)

This test is included in the Toxic Effects Screen (T.E.S.) and high

levels are examined for abnormal enzyme forms using affinity

chromatography. This can identify exposure to toxic metals such as

mercury (from dental amalgam) and cadmium (from smoking).

We sometimes also see a broad enzyme band in the `tail' of the elution

profile. Recent research by ourselves and others has shown this to

indicate increased levels of high molecular weight or protein-bound

organic toxins. This will now be reported when found during a T.E.S.

(fee £55).

Red-cell G.S.T. with affinity chromatography studies are also

available separately [Green top heparin tube] £25.00

Note that the serum G.S.T. is liver-derived and affinity

chromatography can be performed (included in the T.E.S.). Glutathione

conjugation is one of the most important detoxification pathways.

Serum G.S.T. can be requested separately

[Red top clotted tube]

£26.00

Is this the correct test and should I have the red cell GST and/or the

serum GST?

4. If you have a minute, could you please explain what glutathione

transferase does?

With many thanks,

Sheila

-- In , " rvankonynen " <richvank@...>

wrote:

>

> Hi, Sheila.

>

> I, too, appreciate your posting your results.

>

> In regard to the bowel-tolerance dosage of vitamin C, I do think

> that could be beneficial for you. I have only recently come to the

> realization that with very high dosage of vitamin C, the oxidation-

> reduction reaction couple between glutathione and vitamin C can

> actually be reversed, so that vitamin C will regenerate glutathione,

> rather than loading it down and diminishing it. So low dosages of

> vitamin C do not load glutathione, and neither do very high doses

> (which actually help to regenerate it). Intermediate doses,

> however, will tend to load glutathione, as Dr. Cheney originally

> said.

>

> Building up your other antioxidants with the supplements you are

> taking should also be helpful, since the antioxidants work together,

> and it's important to have enough of all of them in the basic

> antioxidant network, which includes glutathione, vitamins E and C,

> alpha lipoic acid, and coenzyme Q-10.

>

> I do suspect that there may be genetic variations in your

> glutathione transferase and glutathione peroxidase enzymes, from

> what you've reported. I don't know if BioLab characterizes them.

> The Genovations DetoxiGenomic Profile does characterize the

> glutathione transferases, but not the glutathione peroxidases. The

> Genova Comprehensive Detoxification profile measures the activity of

> the glutathione peroxidase. I think Biolab may offer a test of the

> activity of the glutathione transferases. If the activities are

> low, that suggests genetic variations. Low activity in glutathione

> peroxidase is most often due to low selenium, because that is a

> cofactor for some of these enzymes.

>

> Glutathione is able to react without the help of these enzymes to

> some degree, so there is value in building it up even if the enzymes

> have problems. Lipoceutical glutathione seems like a promising way

> to do that.

>

> I don't know whether it's possible to have a block in the

> methylation cycle and to still have a normal level of glutathione,

> if the enzymes that use it have genetic variations, but I suspect

> not.

>

> Perhaps trying to get a better idea about how well these supporting

> enzymes are working would be worthwhile. I read in one of Dr.

> 's old newletters that he had found a few patients whose

> glutathione peroxidase was missing one of its four tetramers (a

> large part of the protein molecule), but I don't know if he offers

> such a test routinely or whether it was only a small research

> project that he did.

>

> Rich

[Guest guest]

Hi, Sheila.

>

> Hi Rich,

>

> Very many thanks for your reply. I am really grateful to get your

> insights into all this and for your time.

***You're very welcome. I appreciate learning from your experience

and from your interactions with BioLab.

>

> May I ask you a quick couple of questions relating to your reply?

***Sure.

>

> 1. In the past you recommended a book on antioxidants. I am right

in

> remembering it was " The Antioxidant Miracle " by Lester Packer?

***That's right. It's a small paperback book, and I think it is

about the best for giving the overall picture of how the

antioxidants work. The concept of the antioxidant network is

Packer's. Most of the published research one hears about in the

news considers these antioxidants in isolation from each other, and

I think that's a mistake. It's very important to have enough of

each of them, because they hand off electrons from one to the other,

like a bucket brigade. If one is missing, the whole antioxidant

enterprise suffers. I think that's the origin of the occasional

news reports that say such and such a vitamin isn't really

beneficial for such and such. Often, they just didn't take this

network into account.

>

> 2. I will need to start taking ALA, that is the only one you

mention I

> am not on, and from what you say they all work synergistically.

>

> I have been put off taking it in the past after reading how it

> mobilises mercury and also the DAN book says they found some

autistic

> children developed " severe yeast infections " from taking it.

>

> So is there any way I can avoid these pitfalls?

>

> Also which form of ALA would you recommend, the R lipoic form or

the

> racemic form or would either do?

***Yes, those are pitfalls of taking ALA. If you do have high

mercury levels or gut issues, including a past history of yeast

infections, it might be better not to supplement ALA. It is

synthesized in the body, so you might just depend on endogenous

synthesis. It contains some sulfur, so if you can get chemically

reduced sulfur in another way that won't stimulate yeasts, such as

transdermal glutathione or just animal-based protein in the diet

(meat or eggs, for example), it might be better to do that. If not,

I think the R lipoic form is the best form.

>

> 3. Yes you are right, I have just looked up the Biolab site and I

can

> get the glutathione peroxidase tested.

***O.K. I think you are very fortunate to have Biolab in the U.K.

They do some very good things. If the activity of glutathione

peroxidase comes out low, the first suspect is low selenium. As you

may know, the population of the U.K. has been reported to have gone

down in average selenium levels over the past few years, because the

formation of the European Union has caused more of the grain used

for food in the U.K. to be purchased from Europe than from Canada,

as was done previously. The soils in Europe are lower in selenium

than those in the grain-growing regions in North America. Selenium

is a nonessential mineral for plants, so the crops will grow just as

well without it, but if they are grown on low-selenium soil, they

will have less selenium in them, and will provide less to the people

who eat them.

>

> Here is what they say about their glutathione transferase test:-

>

> Glutathione-S-transferase (G-S-T)

>

> This test is included in the Toxic Effects Screen (T.E.S.) and high

> levels are examined for abnormal enzyme forms using affinity

> chromatography. This can identify exposure to toxic metals such as

> mercury (from dental amalgam) and cadmium (from smoking).

>

> We sometimes also see a broad enzyme band in the `tail' of the

elution

> profile. Recent research by ourselves and others has shown this to

> indicate increased levels of high molecular weight or protein-bound

> organic toxins. This will now be reported when found during a

T.E.S.

> (fee £55).

>

> Red-cell G.S.T. with affinity chromatography studies are also

> available separately [Green top heparin tube] £25.00

>

> Note that the serum G.S.T. is liver-derived and affinity

> chromatography can be performed (included in the T.E.S.).

Glutathione

> conjugation is one of the most important detoxification pathways.

>

> Serum G.S.T. can be requested separately

>

> [Red top clotted tube]

>

> £26.00

>

> Is this the correct test and should I have the red cell GST and/or

the

> serum GST?

***Dr. has written me that he most often sees elevations in

the red cell type of GST to be associated with exposure to toxic

metals, while elevations in the serum type of GST is most often

associated with toxic organic chemical exposure. In both cases, he

sees glutathione depletion. I think this means that the different

types of GST are used primarily for detoxing these corresponding

types of toxins. When glutathione goes down, the body upregulates

the type of enzyme needed to detox the particular toxins present,

which will help to increase the reaction rate to some degree, even

though glutathione is in scarce supply.

***Biolab uses chromatography to analyze the actual GST enzymes,

while Genovations analyzes the variations in the genes that code for

these enzymes. With either approach, abnormalities in these enzymes

can be detected. I think it would be worthwhile for you to have

both the RBC and the serum types of GST analyzed by Biolab. Whether

or not you would want to do this as part of the Toxic Effects Screen

would depend on the cost and whether you already have enough

information about the toxic substances in your body. I always like

to have more data, but I realize that it comes at a price, and I am

not the one paying the bill!

>

> 4. If you have a minute, could you please explain what glutathione

> transferase does?

***Yes. There are several types of glutathione transferase

enzymes. In general, their job is to act as catalysts to join (or

to conjugate) glutathione with toxic substance molecules. This is

one of the so-called phase 2 detox pathways, and it is used for

several categories of toxins, including heavy metals, pesticides,

organic solvents, acetaminophen, and others. After a couple of

additional reactions, this conjugate is called a mercapturic acid.

The mercapturic acids are more water-soluble than the original

toxins (such as heavy metals or pesticides or organic solvents,

etc.) so that they are more easily eliminated into the stools or the

urine. The glutathione transferase enzymes have a further

responsibility to deal with peroxides, such as lipid peroxides,

formed by attack on the lipid membranes of the cells by reactive

oxygen species, such as oxidizing free radicals. This latter is the

main job of the glutathione peroxidase enzymes, but the glutathione

transferase enzymes also have a role in this function. So if a

person has abnormalities in the glutathione transferase enzymes, I

think there can be a tendency toward both buildup of toxins in the

body and toward development of a state of oxidative stress. And, of

course, both are seen in CFS. I think that either a depletion in

glutathione itself or abnormalities in the GST and/or glutathione

peroxidase enzymes can cause this.

> With many thanks,

>

> Sheila

***You're welcome.

***Rich

[Guest guest]

> > Is this the correct test and should I have the red cell GST

and/or

> the

> > serum GST?

>

> ***Dr. has written me that he most often sees elevations in

> the red cell type of GST to be associated with exposure to toxic

> metals, while elevations in the serum type of GST is most often

> associated with toxic organic chemical exposure.

Hi,

I had raised glutathione transferase a few years ago (not had it

remeasured). In me he found it was associated with excess breakdown

of estrogenic tissue and found polypeptides and plus a few traces of

organochlorides bound to the estrogen receptors. I know someone

else with ME who also had glutathione trasnsferase associated with

estrogen tissue breakdown. He didn't know where the peptides came

from, one possiblity was peptides from undigested proteins which had

a similar structure to hormones.

Cheers,

Carol

Cheers,

Carol

[Guest guest]

Hi Rich,

Many thanks again for answering all my questions and the explanation

of glutathione transferases and peroxidases, seems very relevant to my

problems with high levels of lipid peroxides causing problems.

I am definitely going to see about getting these tests done and will

let you knowwhen I get the results.

And I've go the book ordered! It is really cheap so that is good.

I think I will steer clear of the ALA at the minute as I have had

problems with digestion and yeast infections in the past.You suggested

transdermal glutathione as a source of reduced sulphur, would

sublingual glutathione (Source Natural produce one) fit the bill or

glutathione and DMSO to carry it through the skin? Would eating

garlic or taking MSM help with this?

With best wishes,

Sheila

> >

> > Hi Rich,

> >

> > Very many thanks for your reply. I am really grateful to get your

> > insights into all this and for your time.

>

> ***You're very welcome. I appreciate learning from your experience

> and from your interactions with BioLab.

> >

> > May I ask you a quick couple of questions relating to your reply?

>

> ***Sure.

> >

> > 1. In the past you recommended a book on antioxidants. I am right

> in

> > remembering it was " The Antioxidant Miracle " by Lester Packer?

>

> ***That's right. It's a small paperback book, and I think it is

> about the best for giving the overall picture of how the

> antioxidants work. The concept of the antioxidant network is

> Packer's. Most of the published research one hears about in the

> news considers these antioxidants in isolation from each other, and

> I think that's a mistake. It's very important to have enough of

> each of them, because they hand off electrons from one to the other,

> like a bucket brigade. If one is missing, the whole antioxidant

> enterprise suffers. I think that's the origin of the occasional

> news reports that say such and such a vitamin isn't really

> beneficial for such and such. Often, they just didn't take this

> network into account.

> >

> > 2. I will need to start taking ALA, that is the only one you

> mention I

> > am not on, and from what you say they all work synergistically.

> >

> > I have been put off taking it in the past after reading how it

> > mobilises mercury and also the DAN book says they found some

> autistic

> > children developed " severe yeast infections " from taking it.

> >

> > So is there any way I can avoid these pitfalls?

> >

> > Also which form of ALA would you recommend, the R lipoic form or

> the

> > racemic form or would either do?

>

> ***Yes, those are pitfalls of taking ALA. If you do have high

> mercury levels or gut issues, including a past history of yeast

> infections, it might be better not to supplement ALA. It is

> synthesized in the body, so you might just depend on endogenous

> synthesis. It contains some sulfur, so if you can get chemically

> reduced sulfur in another way that won't stimulate yeasts, such as

> transdermal glutathione or just animal-based protein in the diet

> (meat or eggs, for example), it might be better to do that. If not,

> I think the R lipoic form is the best form.

> >

> > 3. Yes you are right, I have just looked up the Biolab site and I

> can

> > get the glutathione peroxidase tested.

>

> ***O.K. I think you are very fortunate to have Biolab in the U.K.

> They do some very good things. If the activity of glutathione

> peroxidase comes out low, the first suspect is low selenium. As you

> may know, the population of the U.K. has been reported to have gone

> down in average selenium levels over the past few years, because the

> formation of the European Union has caused more of the grain used

> for food in the U.K. to be purchased from Europe than from Canada,

> as was done previously. The soils in Europe are lower in selenium

> than those in the grain-growing regions in North America. Selenium

> is a nonessential mineral for plants, so the crops will grow just as

> well without it, but if they are grown on low-selenium soil, they

> will have less selenium in them, and will provide less to the people

> who eat them.

> >

> > Here is what they say about their glutathione transferase test:-

> >

> > Glutathione-S-transferase (G-S-T)

> >

> > This test is included in the Toxic Effects Screen (T.E.S.) and high

> > levels are examined for abnormal enzyme forms using affinity

> > chromatography. This can identify exposure to toxic metals such as

> > mercury (from dental amalgam) and cadmium (from smoking).

> >

> > We sometimes also see a broad enzyme band in the `tail' of the

> elution

> > profile. Recent research by ourselves and others has shown this to

> > indicate increased levels of high molecular weight or protein-bound

> > organic toxins. This will now be reported when found during a

> T.E.S.

> > (fee £55).

> >

> > Red-cell G.S.T. with affinity chromatography studies are also

> > available separately [Green top heparin tube] £25.00

> >

> > Note that the serum G.S.T. is liver-derived and affinity

> > chromatography can be performed (included in the T.E.S.).

> Glutathione

> > conjugation is one of the most important detoxification pathways.

> >

> > Serum G.S.T. can be requested separately

> >

> > [Red top clotted tube]

> >

> > £26.00

> >

> > Is this the correct test and should I have the red cell GST and/or

> the

> > serum GST?

>

> ***Dr. has written me that he most often sees elevations in

> the red cell type of GST to be associated with exposure to toxic

> metals, while elevations in the serum type of GST is most often

> associated with toxic organic chemical exposure. In both cases, he

> sees glutathione depletion. I think this means that the different

> types of GST are used primarily for detoxing these corresponding

> types of toxins. When glutathione goes down, the body upregulates

> the type of enzyme needed to detox the particular toxins present,

> which will help to increase the reaction rate to some degree, even

> though glutathione is in scarce supply.

>

> ***Biolab uses chromatography to analyze the actual GST enzymes,

> while Genovations analyzes the variations in the genes that code for

> these enzymes. With either approach, abnormalities in these enzymes

> can be detected. I think it would be worthwhile for you to have

> both the RBC and the serum types of GST analyzed by Biolab. Whether

> or not you would want to do this as part of the Toxic Effects Screen

> would depend on the cost and whether you already have enough

> information about the toxic substances in your body. I always like

> to have more data, but I realize that it comes at a price, and I am

> not the one paying the bill!

> >

> > 4. If you have a minute, could you please explain what glutathione

> > transferase does?

>

> ***Yes. There are several types of glutathione transferase

> enzymes. In general, their job is to act as catalysts to join (or

> to conjugate) glutathione with toxic substance molecules. This is

> one of the so-called phase 2 detox pathways, and it is used for

> several categories of toxins, including heavy metals, pesticides,

> organic solvents, acetaminophen, and others. After a couple of

> additional reactions, this conjugate is called a mercapturic acid.

> The mercapturic acids are more water-soluble than the original

> toxins (such as heavy metals or pesticides or organic solvents,

> etc.) so that they are more easily eliminated into the stools or the

> urine. The glutathione transferase enzymes have a further

> responsibility to deal with peroxides, such as lipid peroxides,

> formed by attack on the lipid membranes of the cells by reactive

> oxygen species, such as oxidizing free radicals. This latter is the

> main job of the glutathione peroxidase enzymes, but the glutathione

> transferase enzymes also have a role in this function. So if a

> person has abnormalities in the glutathione transferase enzymes, I

> think there can be a tendency toward both buildup of toxins in the

> body and toward development of a state of oxidative stress. And, of

> course, both are seen in CFS. I think that either a depletion in

> glutathione itself or abnormalities in the GST and/or glutathione

> peroxidase enzymes can cause this.

>

> > With many thanks,

> >

> > Sheila

>

> ***You're welcome.

>

> ***Rich

>

[Guest guest]

Hi, Sheila.

>

> Hi Rich,

>

> Many thanks again for answering all my questions and the

explanation

> of glutathione transferases and peroxidases, seems very relevant

to my

> problems with high levels of lipid peroxides causing problems.

***You're welcome.

>

> I am definitely going to see about getting these tests done and

will

> let you knowwhen I get the results.

***O.K., sounds good.

>

> And I've go the book ordered! It is really cheap so that is good.

***O.K.

>

> I think I will steer clear of the ALA at the minute as I have had

> problems with digestion and yeast infections in the past.

***O.K.

You suggested

> transdermal glutathione as a source of reduced sulphur, would

> sublingual glutathione (Source Natural produce one) fit the bill or

> glutathione and DMSO to carry it through the skin? Would eating

> garlic or taking MSM help with this?

***The sublingual glutathione should work. I don't know how well

glutathione and DMSO would work. Garlic should be O.K. MSM might

encourage yeasts.

>

> With best wishes,

>

> Sheila

***Best wishes to you, too.

***Rich