Use of optophoresis as an in vitro predictor of cell response to chemotherapy for chronic lymphocytic leukemia

[Guest guest]

BlankUse of optophoresis as an in vitro predictor of cell response to

chemotherapy for chronic lymphocytic leukemia.

M Nerenberg, I Kariv, P McNeeley, P Marchand, S Sur, J Diver, S Riccitelli, J

Nieva, and A Saven

Leuk Lymphoma, October 1, 2006; 47(10): 2194-202.

Genoptix, Inc., 3398 Carmel Mountain Road, San Diego, CA, 92121, USA

B-cell chronic lymphocytic leukemia [CLL] is characterized by active

accumulation of clonal CD5+/CD19+/CD23+ B cells. Individualized characterization

of patient cell resistance/sensitivity to specific agents can provide important

information to guide therapy selection. We have utilized optophoresis, which is

a technique for the analysis of the motion of cells within a moving optical

gradient field. It detects the broad cellular changes associated with apoptosis

based on physical characteristics of the cell, such as morphology, size,

refractive index, density, and surface properties. We analyzed peripheral blood

samples from 62 CLL patients in the presence of varying concentrations of

chemotherapeutic agents. Optophoresis and a more conventional measurement of

cell death were utilized. The outcome of ex vivo drug resistance using

optophoresis was compared to clinical response in 30 patients for which there

was clinical outcome data available. The overall accuracy of optophoresis in

reflecting clinical response was 80%. It has advantages over alternative methods

of determining chemoresistance including the ability to evaluate very small

sample sizes and ability to work in mixed-cell populations.Changes in cell

physical characteristics in response to chemotherapy, as measured by

optophoresis is an accurate method for predicting chemosensitivity ex vivo in

CLL.

PMID: 17071495