Guest guest Posted September 14, 2005 Report Share Posted September 14, 2005 Thanks for the post--I was not aware of the potential of MS in DNA and will ask around about it when I have a chance. The link you provided certainly makes it sound useful in PCR, detection of polymorphisms, etc. Dr. Vladutiu introduced me to the term " proteomics " several years ago. I have a copy of her paper " Heterozygosity: an expanding role in proteomics. " Mol Genet Metab. 2001 Sep-Oct;74(1-2):51-63. It does not relate to MS at all but if you're interested in a copy, my hubby could scan in for you. My copy is marked up but still legible. As for developing a " fixative " substrate or cofactor for particular deletions, substitutions, etc, I have been dreaming of that for years. I doubt it will happen in my lifetime, but maybe in my son's. In 1983, I was told that gene therapy would be available for me in 20 years, but that hasn't happened either. The notorious gap between theory and reality........ Barbara > I just went to some proteomics talks last night, and it's amazing > what people can do nowadays with mass spectrometry (for those who > don't know, that's a technique that breaks proteins or other > compounds and chemicals up into pieces, then you use a computer > to " assemble " the pieces to figure out what the whole thing looked > like). > > I'm wondering if they could do similar studies on DNA, looking for a > different molecular weight to indicate a problem, then they break it > up further to locate the problem. Sorry if this is too technical for > some, I'm just wondering if the research in DNA MS is advancing like > proteomic (protein study) MS is. > > http://www.nist.gov/sigmaxi/Posters01/Vallone-abst.htm > > I wonder if proteomic MS could be used to determine if and how > Complex I is missequenced - major problem is that it is a protein > complex and so big I guess. Just as a further thought, when they > find an incorrect amino acid in a protein, they could develop a > different substrate (that is, raw material like CoQ10) that might > work for people with that particular substition... > > Take care, > RH > Quote Link to comment Share on other sites More sharing options...
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