Re: mitoldies: muscle biopsy question

[Guest guest]

Thanks, but then what is mitochondrial proliferation? is that the same as

mito dysfunction?

Also one more question if I am deficient in complex II/III how is it that my

complex II is normal , complex III was not measured

[Guest guest]

Thanks, but then what is mitochondrial proliferation? is that the same as

mito dysfunction?

Also one more question if I am deficient in complex II/III how is it that my

complex II is normal , complex III was not measured

[Guest guest]

Citrate synthase is a marker for mitochondrial function. If it is reduced,

it may indicate broad mitochondrial dysfunction. Anything less than 50% may

be a significant reduction. My citrate synthase is about 30%.

B

_____

From: MitomomX3@...

Sent: Wednesday, December 29, 2004 8:20 PM

To:

Subject: : muscle biopsy question

I have a question I have never understood..

On the muscle biopsy it states a level for cintrate synthesis it gives a

percent but not what is normal.

How do you know if you have increased mito proliferation..I read an article

that said someones sintrate synthesis was 52% showing proliferation..

what is normal and what is not as far as that percent goes?

[Guest guest]

Citrate synthase is a marker for mitochondrial function. If it is reduced,

it may indicate broad mitochondrial dysfunction. Anything less than 50% may

be a significant reduction. My citrate synthase is about 30%.

B

_____

From: MitomomX3@...

Sent: Wednesday, December 29, 2004 8:20 PM

To:

Subject: : muscle biopsy question

I have a question I have never understood..

On the muscle biopsy it states a level for cintrate synthesis it gives a

percent but not what is normal.

How do you know if you have increased mito proliferation..I read an article

that said someones sintrate synthesis was 52% showing proliferation..

what is normal and what is not as far as that percent goes?

[Guest guest]

I have no idea what the answer is, but do know that at some point we have to

put our trust in the doctors who can explain it. I know that with things

such as a complex I defect, some doctors feel it has to be below 5% while

others say 10 or even 15. They are learning right beside us and I think some

of them are just as frustrated as we are.

laurie

> From: MitomomX3@...

> Reply-To:

> Date: Wed, 29 Dec 2004 21:20:23 EST

> To:

> Subject: : muscle biopsy question

>

> I have a question I have never understood..

> On the muscle biopsy it states a level for cintrate synthesis it gives a

> percent but not what is normal.

> How do you know if you have increased mito proliferation..I read an article

> that said someones sintrate synthesis was 52% showing proliferation..

> what is normal and what is not as far as that percent goes?

>

>

>

>

[Guest guest]

I have no idea what the answer is, but do know that at some point we have to

put our trust in the doctors who can explain it. I know that with things

such as a complex I defect, some doctors feel it has to be below 5% while

others say 10 or even 15. They are learning right beside us and I think some

of them are just as frustrated as we are.

laurie

> From: MitomomX3@...

> Reply-To:

> Date: Wed, 29 Dec 2004 21:20:23 EST

> To:

> Subject: : muscle biopsy question

>

> I have a question I have never understood..

> On the muscle biopsy it states a level for cintrate synthesis it gives a

> percent but not what is normal.

> How do you know if you have increased mito proliferation..I read an article

> that said someones sintrate synthesis was 52% showing proliferation..

> what is normal and what is not as far as that percent goes?

>

>

>

>

[Guest guest]

Hi , mitochondrial proliferation means more than normal amounts of

mitochondria in the cell. This occurs in some cases of mito and appears as

" ragged red fibers " upon microscopic examination. If you have mitochondrial

proliferation and ragged red fibers, it would be noted on your biopsy

report. I am not aware of any cause and effect relationship between reduced

citrate synthase and mitochondrial proliferation. They would both be

evidence of mitochondrial dysfunction and could both occur in the same

tissue sample. What I know about citrate synthase I have learned from Dr.

Vladutiu and have not done much personal research of the issue. Her standard

line on lab reports regarding citrate synthase is: " Citrate synthase is used

as a marker for mitochondrial content. Respiratory chain enzyme activities

are normalized against citrate synthase activity when its activity is

greater than 1 standard deviation above or below the normal mean. "

Dr. Vladutiu would be much more qualified to answer your question about

complex II/III measurements. All I can say is that the enzyme labeled II/III

is different biochemically from the enzymes labeled II and III. It seems

possible that II/III may be encoded separately in DNA, in which case one

could have a deficiency in II/III without II and III being affected. There

are probably other biochemical scenarios that could produce this result, but

I don't know biochemistry so can't conjecture.

Barbara

_____

From: MitomomX3@...

Sent: Wednesday, December 29, 2004 8:52 PM

To:

Subject: Re: : muscle biopsy question

Thanks, but then what is mitochondrial proliferation? is that the same as

mito dysfunction?

Also one more question if I am deficient in complex II/III how is it that

my

complex II is normal , complex III was not measured

[Guest guest]

Mitochondrial proliferation is just a way of saying lots of mitochondria.

The complex II/III and the others are all different tests. They test for the

combination and individually - I think to give them more information about

what might be going on. My first fresh biopsy (Atlanta) showed a 0% in

complex I with a low II (probably caused by the defect in I). My second

fresh biopsy showed a defect in I and III with a low II. Now the questions

is - do I have a functional defect in both II and III due to the defect in I

or are both I and III affected, causing problems with II). I think it is

just so confusing.

laurie

> From: MitomomX3@...

> Reply-To:

> Date: Wed, 29 Dec 2004 21:51:33 EST

> To:

> Subject: Re: : muscle biopsy question

>

> Thanks, but then what is mitochondrial proliferation? is that the same as

> mito dysfunction?

> Also one more question if I am deficient in complex II/III how is it that my

> complex II is normal , complex III was not measured

>

>

>

>