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> Dear Friends,

> In 1999, they found a vacuole, which Dr. Hirano from Columbia said

was

> very " unusual. " I have read since that vacuoles are common in

> inclusion-body myositis. My endo,who gave me a provisional dx of

mito in

> 1998, still thinks that I may have some form of myositis. But my

> deafness fits the mito pattern much more closely.

Vacuoles (holes) can be found in various muscle diseases. I have

seen reports of vacuoles in lipid storage disorders, McArdles, and

also associated with COX deficient fibers. This in addition to

inclusion-body myositis, which you mentioned. In an abstract of a

2001 review of 113 muscle biopsies in patients with definite MITO or

presumed MITO, vacuoles were not specifically mentioned, but the

study did find increased glycogen deposition and lipid accumulation

in multiple patients, and those excesses can accumulate in vacuoles.

The full-length article (which I have not seen) may clarify whether

the excess glycogen and lipid was associated with vacuoles in those

mito cases.

Hope the biopsy goes well and that you get some useful information.

Barbara

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Barbara

From my work with kids who are taking biology, all cells cantain vacuoles or

empty spaces. I don't know how they might change in disease, but they are

there to start with.

laurie

>

> Reply-To:

> Date: Thu, 07 Oct 2004 18:27:05 -0000

> To:

> Subject: Re: vacuoles

>

>

>

>> Dear Friends,

>

>> In 1999, they found a vacuole, which Dr. Hirano from Columbia said

> was

>> very " unusual. " I have read since that vacuoles are common in

>> inclusion-body myositis. My endo,who gave me a provisional dx of

> mito in

>> 1998, still thinks that I may have some form of myositis. But my

>> deafness fits the mito pattern much more closely.

>

> Vacuoles (holes) can be found in various muscle diseases. I have

> seen reports of vacuoles in lipid storage disorders, McArdles, and

> also associated with COX deficient fibers. This in addition to

> inclusion-body myositis, which you mentioned. In an abstract of a

> 2001 review of 113 muscle biopsies in patients with definite MITO or

> presumed MITO, vacuoles were not specifically mentioned, but the

> study did find increased glycogen deposition and lipid accumulation

> in multiple patients, and those excesses can accumulate in vacuoles.

> The full-length article (which I have not seen) may clarify whether

> the excess glycogen and lipid was associated with vacuoles in those

> mito cases.

>

> Hope the biopsy goes well and that you get some useful information.

> Barbara

>

>

>

>

>

> Medical advice, information, opinions, data and statements contained herein

> are not necessarily those of the list moderators. The author of this e mail is

> entirely responsible for its content. List members are reminded of their

> responsibility to evaluate the content of the postings and consult with their

> physicians regarding changes in their own treatment.

>

> Personal attacks are not permitted on the list and anyone who sends one is

> automatically moderated or removed depending on the severity of the attack.

>

>

>

>

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Tks, Laurie, for this clarification. I'm obviously not a biologist.

I only know that vacuoles have been reported as abnormal pathology

in muscle biopsies and associated with various muscle diseases. The

adjective I've seen most often is " rimmed " but adjectives are not

always used. I'm puzzled as to why any pathologist would report a

vacuole as a significant finding if all cells contain them? Beats

me.

Barbara

> >> Dear Friends,

> >

> >> In 1999, they found a vacuole, which Dr. Hirano from Columbia

said

> > was

> >> very " unusual. " I have read since that vacuoles are common in

> >> inclusion-body myositis. My endo,who gave me a provisional dx of

> > mito in

> >> 1998, still thinks that I may have some form of myositis. But my

> >> deafness fits the mito pattern much more closely.

> >

> > Vacuoles (holes) can be found in various muscle diseases. I have

> > seen reports of vacuoles in lipid storage disorders, McArdles,

and

> > also associated with COX deficient fibers. This in addition to

> > inclusion-body myositis, which you mentioned. In an abstract of a

> > 2001 review of 113 muscle biopsies in patients with definite

MITO or

> > presumed MITO, vacuoles were not specifically mentioned, but the

> > study did find increased glycogen deposition and lipid

accumulation

> > in multiple patients, and those excesses can accumulate in

vacuoles.

> > The full-length article (which I have not seen) may clarify

whether

> > the excess glycogen and lipid was associated with vacuoles in

those

> > mito cases.

> >

> > Hope the biopsy goes well and that you get some useful

information.

> > Barbara

> >

> >

> >

> >

> >

> > Medical advice, information, opinions, data and statements

contained herein

> > are not necessarily those of the list moderators. The author of

this e mail is

> > entirely responsible for its content. List members are reminded

of their

> > responsibility to evaluate the content of the postings and

consult with their

> > physicians regarding changes in their own treatment.

> >

> > Personal attacks are not permitted on the list and anyone who

sends one is

> > automatically moderated or removed depending on the severity of

the attack.

> >

> >

> >

> >

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Barbara

Beats me too!

I've helped my students to make cell models and it always a challenge to

create empty space. Balloons that are little and have just a little air in

them has helped. I guess it is time to pull a biology book off my shelf and

check it out.

laurie

>

> Reply-To:

> Date: Thu, 07 Oct 2004 21:00:44 -0000

> To:

> Subject: Re: vacuoles

>

>

> Tks, Laurie, for this clarification. I'm obviously not a biologist.

> I only know that vacuoles have been reported as abnormal pathology

> in muscle biopsies and associated with various muscle diseases. The

> adjective I've seen most often is " rimmed " but adjectives are not

> always used. I'm puzzled as to why any pathologist would report a

> vacuole as a significant finding if all cells contain them? Beats

> me.

>

> Barbara

>

>

>>>> Dear Friends,

>>>

>>>> In 1999, they found a vacuole, which Dr. Hirano from Columbia

> said

>>> was

>>>> very " unusual. " I have read since that vacuoles are common in

>>>> inclusion-body myositis. My endo,who gave me a provisional dx of

>>> mito in

>>>> 1998, still thinks that I may have some form of myositis. But my

>>>> deafness fits the mito pattern much more closely.

>>>

>>> Vacuoles (holes) can be found in various muscle diseases. I have

>>> seen reports of vacuoles in lipid storage disorders, McArdles,

> and

>>> also associated with COX deficient fibers. This in addition to

>>> inclusion-body myositis, which you mentioned. In an abstract of a

>>> 2001 review of 113 muscle biopsies in patients with definite

> MITO or

>>> presumed MITO, vacuoles were not specifically mentioned, but the

>>> study did find increased glycogen deposition and lipid

> accumulation

>>> in multiple patients, and those excesses can accumulate in

> vacuoles.

>>> The full-length article (which I have not seen) may clarify

> whether

>>> the excess glycogen and lipid was associated with vacuoles in

> those

>>> mito cases.

>>>

>>> Hope the biopsy goes well and that you get some useful

> information.

>>> Barbara

>>>

>>>

>>>

>>>

>>>

>>> Medical advice, information, opinions, data and statements

> contained herein

>>> are not necessarily those of the list moderators. The author of

> this e mail is

>>> entirely responsible for its content. List members are reminded

> of their

>>> responsibility to evaluate the content of the postings and

> consult with their

>>> physicians regarding changes in their own treatment.

>>>

>>> Personal attacks are not permitted on the list and anyone who

> sends one is

>>> automatically moderated or removed depending on the severity of

> the attack.

>>>

>>>

>>>

>>>

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Christie told me today they started studing cells in her biology class.

Too bad they don't have a text book to look in other than one set for

the entire school.

Laureta Fitzgerald wrote:

>Barbara

>

>Beats me too!

>

>I've helped my students to make cell models and it always a challenge to

>create empty space. Balloons that are little and have just a little air in

>them has helped. I guess it is time to pull a biology book off my shelf and

>check it out.

>

>laurie

>

>

>

>>

>>Reply-To:

>>Date: Thu, 07 Oct 2004 21:00:44 -0000

>>To:

>>Subject: Re: vacuoles

>>

>>

>>Tks, Laurie, for this clarification. I'm obviously not a biologist.

>>I only know that vacuoles have been reported as abnormal pathology

>>in muscle biopsies and associated with various muscle diseases. The

>>adjective I've seen most often is " rimmed " but adjectives are not

>>always used. I'm puzzled as to why any pathologist would report a

>>vacuole as a significant finding if all cells contain them? Beats

>>me.

>>

>>Barbara

>>

>>

>>>>

>>>>

>>>>>Dear Friends,

>>>>>

>>>>>

>>>>>In 1999, they found a vacuole, which Dr. Hirano from Columbia

>>>>>

>>>>>

>>said

>>

>>

>>>>was

>>>>

>>>>

>>>>>very " unusual. " I have read since that vacuoles are common in

>>>>>inclusion-body myositis. My endo,who gave me a provisional dx of

>>>>>

>>>>>

>>>>mito in

>>>>

>>>>

>>>>>1998, still thinks that I may have some form of myositis. But my

>>>>>deafness fits the mito pattern much more closely.

>>>>>

>>>>>

>>>>Vacuoles (holes) can be found in various muscle diseases. I have

>>>>seen reports of vacuoles in lipid storage disorders, McArdles,

>>>>

>>>>

>>and

>>

>>

>>>>also associated with COX deficient fibers. This in addition to

>>>>inclusion-body myositis, which you mentioned. In an abstract of a

>>>>2001 review of 113 muscle biopsies in patients with definite

>>>>

>>>>

>>MITO or

>>

>>

>>>>presumed MITO, vacuoles were not specifically mentioned, but the

>>>>study did find increased glycogen deposition and lipid

>>>>

>>>>

>>accumulation

>>

>>

>>>>in multiple patients, and those excesses can accumulate in

>>>>

>>>>

>>vacuoles.

>>

>>

>>>>The full-length article (which I have not seen) may clarify

>>>>

>>>>

>>whether

>>

>>

>>>>the excess glycogen and lipid was associated with vacuoles in

>>>>

>>>>

>>those

>>

>>

>>>>mito cases.

>>>>

>>>>Hope the biopsy goes well and that you get some useful

>>>>

>>>>

>>information.

>>

>>

>>>>Barbara

>>>>

>>>>

>>>>

>>>>

>>>>

>>>>Medical advice, information, opinions, data and statements

>>>>

>>>>

>>contained herein

>>

>>

>>>>are not necessarily those of the list moderators. The author of

>>>>

>>>>

>>this e mail is

>>

>>

>>>>entirely responsible for its content. List members are reminded

>>>>

>>>>

>>of their

>>

>>

>>>>responsibility to evaluate the content of the postings and

>>>>

>>>>

>>consult with their

>>

>>

>>>>physicians regarding changes in their own treatment.

>>>>

>>>>Personal attacks are not permitted on the list and anyone who

>>>>

>>>>

>>sends one is

>>

>>

>>>>automatically moderated or removed depending on the severity of

>>>>

>>>>

>>the attack.

>>

>>

>>>>

>>>>

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Muscle Biopsy and the Pathology of Skeletal Muscle

http://www.emedicine.com/neuro/topic230.htm#target42

This is a peer-reviewed article on muscle biopsies and their

interpretation. It has several references to vacuoles, including an

image, if anyone is interested.

One quote: " Several fibers contain numerous clear vacuoles on this

H&E cross-section from a patient with colchicine myopathy. Vacuoles

can be observed in a variety of situations, including glycogen

storage disease, critical care myopathy, and periodic paralyses.

Vacuoles also are observed as freezing artifact in sections that

have been frozen with poor technique. "

Barbara

> >>>>

> >>>>

> >>>>>Dear Friends,

> >>>>>

> >>>>>

> >>>>>In 1999, they found a vacuole, which Dr. Hirano from Columbia

> >>>>>

> >>>>>

> >>said

> >>

> >>

> >>>>was

> >>>>

> >>>>

> >>>>>very " unusual. " I have read since that vacuoles are common in

> >>>>>inclusion-body myositis. My endo,who gave me a provisional dx

of

> >>>>>

> >>>>>

> >>>>mito in

> >>>>

> >>>>

> >>>>>1998, still thinks that I may have some form of myositis. But

my

> >>>>>deafness fits the mito pattern much more closely.

> >>>>>

> >>>>>

> >>>>Vacuoles (holes) can be found in various muscle diseases. I

have

> >>>>seen reports of vacuoles in lipid storage disorders, McArdles,

> >>>>

> >>>>

> >>and

> >>

> >>

> >>>>also associated with COX deficient fibers. This in addition to

> >>>>inclusion-body myositis, which you mentioned. In an abstract

of a

> >>>>2001 review of 113 muscle biopsies in patients with definite

> >>>>

> >>>>

> >>MITO or

> >>

> >>

> >>>>presumed MITO, vacuoles were not specifically mentioned, but

the

> >>>>study did find increased glycogen deposition and lipid

> >>>>

> >>>>

> >>accumulation

> >>

> >>

> >>>>in multiple patients, and those excesses can accumulate in

> >>>>

> >>>>

> >>vacuoles.

> >>

> >>

> >>>>The full-length article (which I have not seen) may clarify

> >>>>

> >>>>

> >>whether

> >>

> >>

> >>>>the excess glycogen and lipid was associated with vacuoles in

> >>>>

> >>>>

> >>those

> >>

> >>

> >>>>mito cases.

> >>>>

> >>>>Hope the biopsy goes well and that you get some useful

> >>>>

> >>>>

> >>information.

> >>

> >>

> >>>>Barbara

> >>>>

> >>>>

> >>>>

> >>>>

> >>>>

> >>>>Medical advice, information, opinions, data and statements

> >>>>

> >>>>

> >>contained herein

> >>

> >>

> >>>>are not necessarily those of the list moderators. The author of

> >>>>

> >>>>

> >>this e mail is

> >>

> >>

> >>>>entirely responsible for its content. List members are reminded

> >>>>

> >>>>

> >>of their

> >>

> >>

> >>>>responsibility to evaluate the content of the postings and

> >>>>

> >>>>

> >>consult with their

> >>

> >>

> >>>>physicians regarding changes in their own treatment.

> >>>>

> >>>>Personal attacks are not permitted on the list and anyone who

> >>>>

> >>>>

> >>sends one is

> >>

> >>

> >>>>automatically moderated or removed depending on the severity of

> >>>>

> >>>>

> >>the attack.

> >>

> >>

> >>>>

> >>>>

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Dear Barbara,

Thanks for your usual, very useful information. It certainly is

complicated, this disease, but your postings always add some light onto

the subject.

Will keep you posted, and thank you to you and to Laurie for your

conversation about vacuoles.

Maybe there should be some nominations for some lesser form of the Nobel

Prize for medicine being bandied about on the mito lists!

We seem to be forever immersed in some form of research, and most of us

graduated many moons ago.

Take care.

S.

PA

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Dear Barbara,

THANKS so much for the excellent article about muscle biopsies and their

interpretations. It is the best I have ever read, a " keeper. "

Gratefully,

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