Guest guest Posted September 21, 2004 Report Share Posted September 21, 2004 http://www.arthritis.org/research/bulletin/vol51no3/51_3_testing.asp Good summary of testing used to diagnosis muscle disease. May be useful to some of you who are just going through diagnosis. It is written by Dr. Wortmann, whom I happen to know. A wonderful doctor. Pasting the section on muscle biopsy, but there is more. Barbara -------------- Muscle Biopsy Four types of evaluation can be performed on skeletal muscle: histology, histochernistry, electron microscopy, and assays of enzyme activities or other constituents. This combination of histologic and histochemical analysis is generally useful in differentiating myopathic from neuropathic processes. Myopathic changes include rounding and variation of fiber size, internal nuclei, fiber atrophy, degeneration and regeneration, fibrosis, and fatty replacement. Neuropathic conditions that cause denervation produce small, atrophic, angular fibers and target fibers. Reinnervation causes fiber-type grouping -- aggregation of fibers all of the same type. Hematoxylin and eosin and modified Gornori's trichrome stains are used for most histology. The latter stain is useful in identifying ragged-red fibers, typical findings in many mitochondrial myopathies. A wide variety of stains is used for histochemistry. ATPase stains define fiber type. NADH and succinate dehydrogenases reflect the mitochondria. Periodic acid-Schiff stains are used for glycogen, and oil red for lipid. Ultrastructural analysis shows characteristic changes in cases of inclusion body myositis, increased numbers of altered morphology of mitochondria in mitochondrial myopathies, and abnormal glycogen or lipid deposition. Enzyme deficiency states may be identified with appropriate histochemical stains but are best diagnosed by subjecting the tissue protein to assays for the specific enzyme activity. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 24, 2004 Report Share Posted September 24, 2004 Dear Barbara, Once again, you have posted an EXCELLENT treatise for us to read, by Dr. Wortmann. The information is both very clear and very informative. I am going to save this. Thank you so much! Sincerely, S. PA Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 24, 2004 Report Share Posted September 24, 2004 Dr. W would be very pleased to know you found it helpful. He is one of those rare academics who is all about helping patients. Barbara > Dear Barbara, > Once again, you have posted an EXCELLENT treatise for us to read, by Dr. > Wortmann. The information is both very clear and very informative. I am > going to save this. > Thank you so much! > Sincerely, > S. > PA > > > > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 24, 2004 Report Share Posted September 24, 2004 > Can someone please send me the article on DR. W as it was removed from my messages and I don't know why thank you. Donna K > > Date: 2004/09/24 Fri PM 08:16:41 EDT > To: > Subject: Re: link on muscle testing > > Dr. W would be very pleased to know you found it helpful. He is one > of those rare academics who is all about helping patients. > Barbara > > > > Dear Barbara, > > Once again, you have posted an EXCELLENT treatise for us to read, > by Dr. > > Wortmann. The information is both very clear and very informative. > I am > > going to save this. > > Thank you so much! > > Sincerely, > > S. > > PA > > > > > > > > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 24, 2004 Report Share Posted September 24, 2004 Yes, I also missed the reference, and would find it very helpful. Thanks Sunny > > > Can someone please send me the article on DR. W as it was removed > from my messages and I don't know why thank you. > Donna K > > > > Date: 2004/09/24 Fri PM 08:16:41 EDT > > To: > > Subject: Re: link on muscle testing > > > > Dr. W would be very pleased to know you found it helpful. He is one > > of those rare academics who is all about helping patients. > > Barbara > > > > > > > Dear Barbara, > > > Once again, you have posted an EXCELLENT treatise for us to read, > > by Dr. > > > Wortmann. The information is both very clear and very informative. > > I am > > > going to save this. > > > Thank you so much! > > > Sincerely, > > > S. > > > PA > > > > > > > > > > > > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted September 24, 2004 Report Share Posted September 24, 2004 http://www.arthritis.org/research/bulletin/vol51no3/51_3_testing.asp Good summary of testing used to diagnosis muscle disease. May be useful to some of you who are just going through diagnosis. It is written by Dr. Wortmann, whom I happen to know. A wonderful doctor. Pasting the section on muscle biopsy, but there is more. Barbara -------------- Muscle Biopsy Four types of evaluation can be performed on skeletal muscle: histology, histochernistry, electron microscopy, and assays of enzyme activities or other constituents. This combination of histologic and histochemical analysis is generally useful in differentiating myopathic from neuropathic processes. Myopathic changes include rounding and variation of fiber size, internal nuclei, fiber atrophy, degeneration and regeneration, fibrosis, and fatty replacement. Neuropathic conditions that cause denervation produce small, atrophic, angular fibers and target fibers. Reinnervation causes fiber-type grouping -- aggregation of fibers all of the same type. Hematoxylin and eosin and modified Gornori's trichrome stains are used for most histology. The latter stain is useful in identifying ragged-red fibers, typical findings in many mitochondrial myopathies. A wide variety of stains is used for histochemistry. ATPase stains define fiber type. NADH and succinate dehydrogenases reflect the mitochondria. Periodic acid-Schiff stains are used for glycogen, and oil red for lipid. Ultrastructural analysis shows characteristic changes in cases of inclusion body myositis, increased numbers of altered morphology of mitochondria in mitochondrial myopathies, and abnormal glycogen or lipid deposition. Enzyme deficiency states may be identified with appropriate histochemical stains but are best diagnosed by subjecting the tissue protein to assays for the specific enzyme activity. --- End forwarded message --- Quote Link to comment Share on other sites More sharing options...
Recommended Posts
Join the conversation
You are posting as a guest. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.