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PRODUCT # FSAA

AANGAMIK DMG

INGREDIENTS:

125mg pure sub-lingual N,N-Dimethylglycine

AANGAMIK DMG is the only pure DMG product which contains no fillers

or binders and is therefore pure and non-allergenic.

BENEFITS:

Enhances biologically important cycles in the body

Improves the nutritional and environmental value of the cell

Prevents lactic acid build up, improves recovery time after exercise

Increases oxygenation

Immune strengthening

APPLICATION:

Two a day, or as recommended by your health care practitioner.

AANGAMIK can be swallowed, chewed or taken sublingually for

enhanced absorption. It's naturally pleasant tasting. For best

results, let melt under tongue on an empty stomach.

DMG is hypoallergenic and nonmutagenic. Thousands of clinicians

and doctors have been recommending DMG for 20 years without adverse

or negative side effects. DMG will not affect any nutritional

program you may now be on.

PRECAUTIONS and CONTRAINDICATIONS:

N,N-Dimethylglycine is a completely safe, non-toxic food substance.

Since it is a water soluble nutrient, DMG does not build up in the

body.

ADDITIONAL COMMENTS:

DMG can be used by all health-aware individuals and sports

participants. Care-givers of autistic people should consult their

health care practitioner regarding the therapeutic uses of DMG.

Certain studies have shown that DMG is beneficial for autistism and

those afflicted with obsessive/compulsive behavioral disorders.

Dimethylglycine (DMG) is a nutrient which is found in the cells of

all plants and animals. Biochemists classify DMG as an Intermediary

Metabolite, but in simpler terms, it may also be called a food.

The human body produces the amino acid derivative DMG only in very

small amounts. Scientific research has shown that supplementing the

diet with higher levels of DMG greatly improves the nutritional

environment of the cell.

AANGAMIK DMG was the first DMG product produced in America and as

such has set the standard in the health industry for quality and

purity of formulation.

Dimethylglycine has been found effective in enhancing the body's

immune system. FoodScience Laboratories has been issued five U.S.

Patents for N,N-Dimethylglycine's value and use in improving both

humoral and cellular mediated immunity and for benefiting arthritis.

Although the human body produces DMG, research has shown that

supplementing the diet with N,N-Dimethylglycine enhances a number of

biologically important cycles in the body while improving the

nutritional environment of the cell. It also helps your muscles

utilize nutrients to create energy.

Sports participants and their coaches, nutritionally oriented

doctors and health-conscious individuals have been using AANGAMIK

DMG for over 20 years.

ARE ALL DMG SUPPLEMENTS THE SAME?

NO, they are not. N,N-Dimethylglycine is a difficult nutrient to

produce in high quality supplement form. The purest, most effective

N,N-Dimethyl- glycine product currently available is AANGAMIK DMG

from Food Science Laboratories. FoodScience's AANGAMIK DMG was the

first DMG product produced in America and as such has set the

standard in the health food industry for quality and purity of

formulation. AANGAMIK DMG is the only pure DMG product produced

with no fillers or binders.

TECHNICAL BULLETIN

N, N-Dimethylglycine™ (DMG)

The Metabolic Enhancer that boosts the immune system, enhances

performance and improves cardiovascular function.

Presented by: V. Kendall, Ph.D.

Vice-President of Research & Development

The benefits of supplemental DMG are many:

Reduces Physical and Environmental Stress

Improves Oxygen Utilization

Enhances Liver Function

Aids Cardiovascular Functions

Optimizes Athletic Performance

Improves the Immune Response

Helps Improve Neurological Function

Possesses Anti-Inflammatory Properties

Enhances Anti-Viral, Anti-Bacterial and Anti-Tumor Defenses

Introduction

N, N-Dimethylglycine (DMG) is a versatile nutrient, accessory food

factor and intermediary metabolite that can enable a person or

animal to function at more optimum mental and physical level and aid

the body in overcoming a number of adverse health conditions. DMG is

an intricate part of human metabolism and has been used in the

health field as a nutritional supplement for over 25 years.

As a nutritional supplement, Dimethylglycine can improve physical

and mental performance by helping the body adapt to the various

forms of stress. Athletes have been using DMG for years to improve

stamina, reduce muscle cramping and enhance recovery from intense

workouts. University research and clinical studies demonstrates that

DMG may be beneficial in helping the body overcome a number of

health problems including cardiovascular disorders, inflammation,

tumors, chronic fatigue syndrome and autism. DMG may even retard the

aging process because of its ability to reduce metabolic stress

caused by poor oxygen availability (hypoxia), free radical damage

and a weakened immune system. DMG can enhance the healing process

for most health disorders as well as promote greater vitality,

energy and performance.

Background

Dimethylglycine was widely studied in the Soviet Union in the `60's

as part of the Russian formula known as Calcium Pangamate. Their

studies indicated that DMG was beneficial to athletic performance,

cardiovascular function, detoxification, skin problems and

neurological function. In one study, DMG was shown to protect the

immune function of guinea pigs exposed to intense radiation. DMG was

found to aid cardiovascular function by reducing angina, high lipid

and cholesterol levels, and high blood pressure as well as improve

circulation to the extremities of the body. The Soviet research on

their DMG based formula demonstrated three basic properties:

Lipotropic effect (to protect the liver from fat infiltration)

Stimulation of oxygen utilization

Detoxifying capacity

Later work done in the United States began to confirm many of the

Russian studies. One of the first areas where DMG was found to give

significant improvement was in horse racing. Veterinarians and

trainers found that DMG could reduce lactic acid buildup and improve

racing times in equine events. Improved recovery times from

intensive training and racing were also noted.

The clinical and nutritional benefits of DMG were also quickly

recognized, especially in the areas of cardiovascular disease and

weakened immunity associated with most degenerative conditions. In

several studies, DMG was found to have anti-seizure activity

(epilepsy), to provide major improvement in autism and to reduce

toxicity to several potent pathogens. Details of this work will be

covered later.

Areas of Possible DMG Application

Laboratory research has indicated that Dimethylglycine can be used

as a nutritional adjunct in a number of health related areas

including:

Cardiovascular Problems

Glucose Metabolism

Inflammatory Conditions and Autoimmune Disorders

Allergies

Chronic Fatigue

Tumors

Liver Disorders

Alcoholism and Drug Addiction

Respiratory Problems

Immune Response Deficiencies

Infections

Neurological Disorders

Autism

Sports Practice

In their book, Prescription for Nutritional Healing, Dr. Balch

and Phyllis Balch, C.N.C., recommend DMG for over 35 different

ailments.

What is Dimethylglycine?

N, N-Dimethylglycine is an amino acid, specifically the dimethylated

derivative of Glycine:

DMG is a normal, physiologically active nutrient found in low levels

in such foods as cereal grains, beans and liver. DMG is also

produced in the body from Choline and Betaine as a cellular

intermediate. The liver rapidly converts DMG into other useful

metabolites via a process known as oxidative dimethylation. By this

process, a methyl group of DMG can be made available for

transmethylation reactions, a pathway which is essential to the

production of many vital products of the cell. Dr. J.W. Meduski of

the University of Southern California has called DMG a " metabolic

enhancer " because of the many ways DMG can improve cellular

metabolism, especially under conditions of distress in the body.

DMG and the Transmethylation Process

By its ability to give up its methyl groups to help in the

production of sulfur-adenosyl-methionine (SAMe), DMG supports

transmethylation processes in the body. Transmethylation is a

reaction where a methyl group (CH3) is transferred from one molecule

to another. It is a biochemical process which is essential to life,

health and regeneration of body cells.

Vitamins, hormones, neurotransmitters, enzymes, nucleic acids (DNA,

RNA) and antibodies depend upon the transfer of methyl groups to

complete their synthesis. SAMe is the active transmethylating agent

and is involved in over 41 different transmethylation reactions.

DMG gives up its methyl groups by a process known as oxidative

demethylation. The one carbon units produced from Dimethylglycine

can be used to form the essential amino acid Methionine from

Homocysteine. This transformation requires folic acid, NAD+, FAD and

Vitamin B12. Methionine in turn is used to produce S-

Adenosylmethionine (SAMe), the active transmethylating agent in the

body.

DMG can therefore act as an indirect methyl donor and function as an

efficient " methionine pump " by converting excess Homocysteine

molecules to Methionine. This process keeps the cells of the body in

a high state of transmethylation potential. It also reduces the

concentration of Homocysteine in the blood, which tends to build up

when there is a low availability of methyl groups. High levels of

Homocysteine have been shown to cause arteriosclerosis in

experimental animals. Dr. Kilmer McCully, a pathologist, has

suggested that Homocysteine is a cause of arteriosclerosis in

humans. DMG may play an important role in keeping Homocysteine at a

safe level.

Besides generating one carbon units, Dimethylglycine generates two

carbon molecules such as Glycine, Serine and the ethanolamines, all

of which are beneficial to the life of the cell. For example,

Glycine functions as an important inhibitory neurotransmitter of the

central nervous system and is used to produce phosphocreatine, a

high energy phosphate molecule used in muscle tissue and in the

tissue of the central nervous system.

The metabolic role of DMG as a supplier of both one and two carbon

molecules to the cell, as well as its ability to contribute to the

formation of S-Adenosylmethionine (SAMe) and subsequent

transmethylation reactions, may help explain DMG's broad metabolic

activity and therapeutic effect on the body.

Dosage

FoodScience of Vermont's DMG comes as a pure 125 mg sublingual

tablet, which is an ideal dose for DMG. By dissolving under the

tongue, the DMG can be absorbed directly into the body, bypassing

the liver. This gives a faster and more efficient use of the DMG.

Depending on the specific area of use, the recommended dosage of DMG

can range from 125 mg to over 1000 mg per day. In most cases it is

best to take DMG several times throughout the day in order to

maintain a more consistent availability to the body. Individuals

with heavy work schedules (stress), athletes or people dealing with

a major health problem can benefit from higher intakes of DMG.

Absorption and Storage

Dimethylglycine is very effectively absorbed from the digestive

tract, including the oral cavity. Sublingual ingestion of DMG

provides effective and rapid absorption, effects of which are

frequently evident within 20 minutes after taking the product.

The amino acid transfer complex for transferring DMG across the

intestinal wall has been identified and is reported. All indications

are that DMG is metabolized rapidly by the liver into one and two

carbon units and therefore the body does not store appreciable

amounts of the nutrient.

Safety Studies on Dimethylglycine

Dimethylglycine has been found to be an extremely safe food

substance as demonstrated by a series of animal studies conducted at

the Medical University of Southern California. Meduski has reported

that Dimethylglycine Hydrochloride has an LD50 (lethal dose to 50%

of the animal population) of 7,400 mg per kg. of animal body weight

in the rat. This amount is generally regarded as nontoxic.

Dimethylglycine is a water soluble nutrient and the enzyme system in

the body effectively converts the substance into metabolites that

are either used by the body or are safely excreted from the body.

Feeding studies at reasonably high levels have demonstrated DMG's

long term safety. A two year feeding study in rats produced no

health problems, even when fed at a level 1/10 the LD50 on a daily

basis (740 mg/kg of body weight per day).

Dimethylglycine is a safe food substance that has been used by

thousands of clinicians and doctors for almost three decades without

adverse or negative side effects.

The Role of N, N-Dimethylglycine in Health

Research has shown that DMG is a physiologically active nutrient

that:

Can significantly improve physical and mental performance.

Can improve and stimulate oxygen utilization and thereby reduce

hypoxic (low oxygen) states in the body.

Can increase resistance to disease and infection by strengthening

both arms of the immune system, including antibody, lymphocyte and

cytokine production.

Aids in detoxification, especially in the liver.

Can cause marked improvement in patients with circulatory

insufficiency and angina pectoris.

May improve glucose metabolism in athletes, diabetics and

hypoglycemics.

May reduce elevated cholesterol, triglycerides and improve

circulation.

May lower high blood pressure.

Reduces training fatigue and shortens recovery periods after

strenuous exercise.

Increases physical and mental energy levels by reducing lactic acid

build-up during periods of stress.

Can retard cataract development in laboratory animals.

Can improve mental alertness and brain function.

Has lipotropic properties that enhance liver function.

Can act as an indirect methyl donor and provide useful building

units for the biosynthesis of vitamins, hormones, neurotransmitters,

antibodies, nucleic acids and metabolically active molecules like

Choline, Creatine and Methionine.

Sports Practice and Athletic Use

The value of DMG as a nutrient and metabolic enhancer was first

observed in the area of athletic performance. DMG has long been used

by athletes to improve overall performance and endurance, to enhance

oxygen utilization and to improve recovery of muscles after

strenuous exercise. It has been shown in a number of athlete and

animal studies over the past 20 years that DMG specifically improves

stamina and cardiovascular function in the body.

Pipes has reported in the Physicians and Sports Medicine

Journal that Dimethylglycine significantly improved performance in a

group of track and field athletes. The DMG group showed an increase

of 27.5% in VO2 Max and of 23.6% in time to exhaustion as compared

to the placebo group. DMG's apparent ergogenic (energy enhancing)

effects could be due to a number of factors, including better oxygen

utilization and cellular respiration, reduced lactic acid build-up,

and enhanced carbohydrate and lipid metabolism.

A 1982 study by Levine and Myhre and published in Equine Practice

showed DMG's rapid ability to reduce a horse's lactic acid level as

compared to controls. The authors reported over a five-fold decrease

in lactate level buildup for the test animals as compared to

controls.

Cator, DVM at the Panhandle Regional Veterinary Clinic, which

confirmed the results of the Equine Practice study, did another

study. Thoroughbreds were checked for speed and blood lactate levels

with and without DMG at 210 heartbeats per minute. The results show

greater speed and a lower blood lactate level when DMG was part of

the horse's diet.

The Effects of N,N-Dimethylglycine on Blood Lactate Levels and Speed

in Racing Thoroughbreds

--Field work conducted at training track at Panhandle Regional

Veterinary Clinic in Spearman, Texas, by R. L. Cator, Jr., DVM

--Laboratory work performed by Texas A & M Medical Veterinary

Diagnostic Laboratory, Amarillo, Texas by Halliburton, Ph.D.

Potter and Moffitt also reported on DMG's lactic acid lowering

effect in a study which they did in 1985 at Texas A & M. When horses

were supplemented with DMG at the rate of 1.6 mg/kg, there was a

significant reduction in blood lactic acid concentration after

strenuous exercise on a treadmill. This dosage equates to

approximately one serving of 750 mg of DMG for a 1,200-1,400 lb

horse.

The mechanisms of how DMG improves oxygen utilization and reduces

lactic acid accumulation are not clear, but its effectiveness in

improving performance and recovery time from strenuous exercise is

well established. This is supported by the paper published in Canine

Practice by Cannon and Kendall showing that racing greyhounds that

were supplemented with DMG had improved racing times and better

recovery from the previous day's training or racing.

Meduski and co-workers at the University of Southern California

conducted experiments with rabbits to monitor the effect of

Dimethylglycine on lactic acid production under hypoxic conditions.

New Zealand White rabbits were exposed to severe surgical stress, a

condition known to increase blood lactate in animals. Those animals

that received DMG showed markedly decreased levels of lactic acid in

their blood compared to control.

Meduski also reported improved oxygen uptake of rats which were on

Dimethylglycine supplementation and exposed to low oxygen (8%)

environment. The DMG fed rats showed better adaptation to hypoxia

than did the controls by actually utilizing more oxygen from the low

oxygen environment.

A New York City marathon club under the direction of Dr. Null

did a six-month study, where they carefully compared the use of DMG

in a marathon to a previous race where it was not used. The

improvements were impressive. In the second race the runners took

500 mg DMG before the race and then followed by 250 mg for every

four miles covered. The marathoners reported much less exhaustion as

compared to the previous race. Based upon compiled statistics,

better race times were obtained along with improved stamina, muscle

and body recovery, and especially the reduction of cramping and

fatigue from the 18th to the 26th mile mark in the marathon.

The evidence that DMG can enhance the performance of athletes is

quite strong. The research, along with actual field evaluation,

shows that DMG is beneficial to endurance athletes (runners, team

sports) as well as short-timed events (weight lifters, sprinters).

Not only will DMG help in overall performance and recovery, but it

will also boost the immune defense of the individual athlete and

make them less susceptible to infections or other illnesses.

Immune Response Enhancement

An individual's immune response is a person's defense against

foreign substances (antigens, pathogens). Its role is to seek out,

identify and destroy those invading substances, which can cause

disease or health problems. The immune system is made up of

different cell types (B-cells, T-cells, macrophages) immune

complexes and molecular products of B and T cells (antibodies from B-

cells, cytokines from T-cells). Macrophages are large scavenger

lymphocytes (white blood cells), which engulf and kill the invader.

Antibodies are proteins which react with specific germs and alert T-

cells (lymphocytes) to identify and attack the foreign substance.

Together these cells and molecular products work to destroy and

eliminate cancer cells, bacteria and viruses which are disease

causing. Factors that weaken the immune system and decrease the

immune response include age, metabolic or genetic disorders,

microbial infestations, stress, nutritional deficiencies, many drugs

and most degenerative diseases. The stronger the immune system, the

greater the immune response will be against disease causing agents.

This results in fewer symptoms of infection and greater probability

of avoiding sickness.

A strong immune system is an important part of staying healthy,

avoiding serious illness and fighting off exposure to pathogens that

may occur. Dimethylglycine (DMG) is a nutritional factor that can

strengthen a person's immune system and bring about a more effective

response against any antigen (foreign substance). Dr. Graber

and his research team first discovered this in 1978 at the Medical

University of South Carolina.

The results of this research, which were published in 1981 in the

Journal of Infectious Diseases, centered on three areas:

DMG invoked a humoral or antibody response in rabbits given typhoid

vaccine demonstrating B-cell activity.

DMG increased lymphocyte population in humans using a Lymphocyte

Blast Transformation test on blood samples of 75 individuals,

including those with diabetes and sickle cell anemia.

A double-blind study involving 20 human subjects showed DMG to be

effective in stimulating both a humoral (antibody) as well as a

cellular-mediated immune response when pneumovax vaccine was

administered as a challenge. A 4.5-fold increase in antibody titer

was seen in the test group as compared to the control group. The

Leukocyte Inhibition Factor (LIF) increased significantly in those

individuals given DMG.

In summary, the research showed that DMG significantly stimulates B-

cells to produce higher antibody responses (humoral branch) and

potentiates a greater activity of T-cells and macrophages (cellular

immunity branch). DMG therefore enhances both arms of the immune

response. In a test-tube evaluation, DMG was shown to return below

par lymphocyte activity to near normal in patients with diabetes or

sickle cell anemia. Patients with these diseases suffer more

infections than do healthy people.

As a result of this work FoodScience Corporation was granted a

patent on DMG and its role in potentiating the immune response (U.S.

Patent #4, 631,198) in 1983.

Research completed in the latter part of 1986 at Clemson University

confirmed the earlier work done at the Medical University of South

Carolina that DMG does indeed potentiate both arms of the immune

response system. Rabbits given either a typhoid or influence antigen

produced a 3-5-fold increase in antibody levels as compared to

control rabbits. T-cell proliferation was also seen in the DMG fed

animals which showed from 3 to 9 times greater levels of T-cells as

compared to controls.

The researchers at Clemson also found that DMG was effective in

increasing interferon production in rabbits. A 2-fold increase in

interferon production was seen in those animals receiving DMG.

Interferon is a cytokine, a product of T-cells, which can act as an

antiviral and anti-tumor agent.

In a study done by the U.S. Army by Dr. Ivin at the Medical

Institute of Infectious Diseases at Ft. Detrick, land evaluated

the effects of DMG with guinea pigs given an anthrax vaccine. No

significant increase in antibody titers were seen in the DMG fed

animals as compared to the vaccinated controls. However, when the

animals were subsequently challenged with a potentially lethal dose

of virulent anthrax bacilli, 50% of the control animals given the

vaccine died. Among the vaccinated animals given the vaccine and

DMG, not one of the DMG fed animals died. These remarkable findings

seem to indicate that although no increase in antibody titers were

seen in the DMG fed animals as compared to the controls, the DMG fed

animals demonstrated an enhanced immunity which must have been due

to an enhanced cellular response (T-cells, B-cells).

The use of DMG in humans and animals offers a safe oral immune

enhancing nutrient, which can offer increased resistance to and

recovery from infectious diseases. Upper respiratory problems

respond well to DMG supplementation. These studies indicate that

people who use DMG may have greater protection from bacterial and

viral infections (flu, colds). This is very important to the

geriatric population who may not even respond to a flu shot because

the immune system is unable to increase antibody production as in

younger people. Anyone with a degenerative condition has a

compromised immune system and would benefit from supplemental DMG.

Cardiovascular System

Dimethylglycine supplementation has been found very beneficial for

circulation and cardiovascular problems. DMG enhances oxygen

delivery to the heart and improves several characteristics of the

blood.

According to a symposium conducted in Moscow in 1964 (USSR Academy

of Sciences and the Institute of Biological and Medical Chemistry),

the principal field of DMG application in trials conducted at over

20 clinics was in the treatment of cardiovascular disorders. The DMG

formula was marketed as Calcium Pangamate, but careful assessment of

the patent and scientific literature has demonstrated that

Dimethylglycine was the active ingredient in the orally administered

product.

The studies reported major improvements in the areas of

arteriosclerosis, artherosclerosis, coronary circulation and

myocardial function, angina pectoris and high blood pressure upon

administration of DMG.

Good results were obtained on daily doses of Dimethylglycine from 50

mg to 90 mg. The principal mode of action to which the improvements

were ascribed centered on DMG's ability to decrease hypoxia (low

oxygen availability) to the various tissues, to improve various

oxidative processes in the body, and to normalize lipid and

carbohydrate metabolism.

A four-year clinical evaluation of Dimethylglycine by

Pries, M.D. of Palmdale, CA, has confirmed the Soviet findings. In

trials involving administration of DMG to over 400 cardiovascular

patients over a four-year period, Dr. Pries reported major

improvements in the following areas:

Increased feeling of well-being.

Improvement in circulatory insufficiency.

Decrease in elevated cholesterol and triglycerides.

Reduction in angina pain.

Fewer arrhythmias.

Decrease of high blood pressure.

Improvement in heart response to stress tests.

Most of the patients in the study had cholesterol levels of greater

than 250+ mg/dl. After taking 250 mg of DMG for 3 months, most

showed a drop in blood cholesterol to approximately 200 mg/dl. A

major drop was seen for the triglycerides as well. The patients

underwent standard diagnostic testing including blood chemistries,

electrocardiograms, and Doplar blood vascular readings .

Dr. Pries concluded that 125 mg of DMG, taken twice daily, was

effective in producing major positive responses in his

cardiovascular patients.

Carbohydrate Metabolism

There is good evidence that DMG may enhance carbohydrate metabolism.

There have been many individual reports from physicians who have

attested to DMG's value in lowering blood sugar levels in diabetic

patients. Problems resulting from impaired circulation, including

gangrene, pain in the lower extremities, wounds and ulcers on the

legs and feet, all responded well to DMG supplementation.

To evaluate Dimethylglycine's possible metabolic effect on Diabetes

Mellitus, an animal study was conducted on a diabetic strain of mice

at the University of Bridgeport. The strain of biologically

engineered diabetic mice chosen for the study exhibited

characteristics similar to diabetes in man: hyperglycemia (high

blood sugar), obesity, glycosinea (sugar in the urine) and

morphological changes in the pancreas.

In two such experiments using controls, the experimental mice were

given a solution of 740 mg of DMG per kg. of body weight, whereas

the control group received just water. Diet and other environmental

factors were kept the same. After statistical analysis, it was shown

that the DMG fed group had significantly lower blood glucose

concentrations and lower weight gains (20-25% decrease) as compared

to the control group.

This animal study confirms DMG's role in improving glucose

metabolism. DMG appears to stimulate tissue oxygen consumption and

improve oxidation at the cellular level. It is known that DMG will

reduce lactic acid build-up under conditions of physical stress,

also indicating that DMG does promote cellular respiration. This may

explain Dimethylglycine's beneficial effects on diabetic patients.

Dr. Mason's book, The Methyl Approach to Hypoglycemia

discusses the effects of stress and lactic acid on hypoglycemia, and

underlines the need for available methyl groups (CH3) to support

liver, adrenal and pancreas functions. DMG acts as a source of

methyl groups via methionine synthesis.

Stanley and Schlenk have reported that all adrenal hormones are

dependent on Methylation for their synthesis. A shortage of methyl

transfer activity can lead to poor hormone production and impaired

glucose metabolism. Lactic aid levels are also known to be higher in

hypoglycemic patients due to poor cellular respiration.

Dimethylglycine has proven beneficial for hypoglycemics because it

assists in reducing elevated lactic acid levels and supplies a rich

source of methyl groups for transmethylation reactions in the body.

Detoxification and Liver Support

Dimethylglycine is beneficial in support of liver metabolism and

detoxification. By improving oxygen utilization, DMG can help

eliminate hypoxia to the tissues and aid in the elimination of

toxins from the blood stream. Dr. Carl Pfieffer of the Bio-Brain

Center in Princeton, NJ reported success in using DMG to reduce

elevated histamine levels in certain patients. This is probably

achieved by increasing the transmethylation pathway and making more

methyl groups available for detoxification of neuro toxic amines.

Patients undergoing either chemical or drug detoxification would

benefit from DMG supplementation (500 to 1000 mg) to improve

cellular metabolism for increased energy levels and improved

detoxification.

Chelation Therapy

Many physicians who utilize Chelation Therapy with their patients

recognize the value of DMG for improving oxygen uptake and

circulation to the extremities of the body. During the chelation

process, DMG supplementation can enhance cellular efficiency by

stimulating the liver and carbohydrate metabolism. Patients receive

a boost in their sense of well being and energy levels which may

relate to improved blood glucose levels. Individuals undergoing

chelation, either for atherosclerosis, alcoholism, diabetes or

sexual dysfunction would benefit from supplementation of 250 to 500

mg of DMG daily.

Cataract Prevention

A study was done at the Pacific University College of Optometry to

evaluate any effect that DMG might have on the Posterior

Subcapsulation Cataract Progression in rats. DMG was applied daily

to the eyes of 10 rats and cataract progression was monitored at

weekly intervals for an eight-week period and compared to untreated

controls. Lens opacities were graded according to apparent size,

location and density. After eight weeks statistical comparison

showed that in the rats receiving DMG, cataract development was

found to be significantly slower than in those rats receiving saline

solution. The mechanism of how DMG retards cataract progression may

be associated with its ability to increase oxygen uptake by hypoxic

tissues. The study suggests the potential use for Dimethylglycine in

the prevention of cataracts either as eye drops or in supplemental

oral administration.

Neurological Support

A lack or imbalance of neurotransmitters can cause a whole series of

brain and nerve dysfunctions. Because Dimethylglycine can act as a

precursor to a number of amino alcohols and acids which aid brain

function, including Glycine and Dimethylethanolamine, its value to

the body's neurological system should not be overlooked.

Dr. Freed of Saint Hospital in Washington D.C.

confirmed in several patients the ability of DMG to prevent or

reduce epileptic seizures. Veterinarians have reported good success

in preventing seizures in dogs and cats using DMG. In another study,

Ward, et al, found that DMG significantly reduced mortality

associated with penicillin-induced seizures in rats. Only one rat

died in the DMG-treated group, whereas 18 died in the control group.

Drs. Roach and Carlin of the Bowman Gray School of Medicine reported

in the New England Journal of Medicine (October, 1982) a case

history where DMG was effective in controlling epileptic seizures in

a 22 year old man who had a long history of mental retardation and

mixed complex, partial and gran mal seizures. Before treatment the

patient was experiencing 16 to 18 generalized seizures per week,

even when on therapeutic levels of phenobarbitol and carbamazepine.

After taking 90 mg of DMG twice daily for one week, the number of

seizures was reduced to three per week. Two attempts to eliminate

DMG supplementation resulted in a dramatic increase in seizure

frequency for the individual.

It may be that DMG is able to cross the blood brain barrier better

than Glycine, thereby acting as a source of the Glycine

neurotransmitter in the brain. Several other explanations are also

possible. The monitored improvement on other epileptic patients has

not been as dramatic as the case cited, but several other instances

of DMG aiding in neurological and seizure-type patients have been

report by Gasion and .

Individuals who are prone to panic attacks and increased anxiety

tend to have increased lactic acid levels which can affect brain

chemistry. By preventing the buildup of lactic acid levels in the

blood, DMG may be effective in reducing panic attacks and

normalizing the stress response in these individuals.

B-16 Melanoma Studies:

Work done by Dr. Lawson at Clemson University strongly suggests that

DMG may have anti-tumor properties as a result of DMG's ability to

enhance the immune response. This was shown in a mouse study using a

B-16 melanoma model. Melanoma is a highly metastatic form of skin

cancer that spreads easily to other organs. Mice receiving DMG had a

significantly higher antibody count against the B-16 melanoma

antigen and the growth of their tumors was significantly retarded as

compared to the controls. The DMG mice lived longer than the

controls and had fewer palpable tumors. One of the more important

findings, however, was that DMG inhibited or prevented the primary

tumors from spreading (metastasis) to vital organs. Histological

examination of the control and test mice showed that metastasis to

vital organs occurred only in the B-16 control group that did not

receive DMG. At the end of the study, all of the control mice had

died but of those mice receiving DMG, 71% were still surviving. DMG

also exerted a strong anti-tumor effect against melanoma in a CAM

(chlorioallantoic membrane) chick embryos assay. In this assay, DMG

reduced tumor growth by 60% as compared to controls. It is now known

that DMG increases the production of Tumor Necrosis Factor-alpha

(TNF-a) which enhances the ability of the body to fight against

cancer. As a result of this work a U.S. patent was granted to

FoodScience Corporation for DMG in treating melanoma. These studies

indicate that DMG may give important nutritional support to the

immune system against some forms of cancer. FoodScience Corporation

is currently funding studies in this area.

Autism

There is a growing body of evidence that DMG is beneficial for

individuals with autism. Autism is a biological brain disorder of

unknown causes that results in a wide range of puzzling and

disturbing social and personal behavior patterns. Two studies have

demonstrated that DMG can modify and improve the behavior, social

interaction, verbal communication and disturbing activities of

autistic children. Significant improvements were seen.

The first study was conducted in 1990, by Dr. Lee Dae Kun, Director

of the Pusan (Korea) Research Center on Child Problems. In this

study, 39 autistic children, ages three to seven were given DMG from

125 mg to 375 mg per day, depending on weight, for a three-month

open trial period.

Resulting Benefits of Dr. Kun's Study

AUTISM & DMG 1

Several children had difficulty sleeping and were more active during

the first few weeks, but this situation improved as the study

progressed. The improvement in the children was especially noted by

the parents.

A second study was completed in 1997 in Taiwan by Dr. Jung of the

Taipei Springtide Foundation. Unlike the first study, done in Korea,

this was a double-blind placebo controlled study involving 84

autistic children who were divided into a DMG TEST GROUP (46) and a

PLACEBO GROUP (38). The DMG TEST GROUP showed statistically

significant improvement on all five ABC scales used to evaluate

effectiveness: Irritability, Lethargy, Stereotypy, Hyperactivity and

Inappropriate Speech. The PLACEBO GROUP showed improvement only on

the lethargy scale. These results confirm the hundreds of

communications that the Autism Research Institute, in San Diego, CA,

under the direction of Dr. Bernard Rimland, have received from

excited parents who have seen remarkable changes in their autistic

children after using DMG. Areas where parents noted improvement

include:

Better Verbal Communication

Better Eye Contact

Improved Loving Attitude

Better Social Interaction

There are a number of possible explanations as to why the use of DMG

has resulted in such remarkable improvement. These include better

oxygen utilization, reduction of lactic acid formation and a

possible decrease in potential seizure activity. Perhaps the most

relevant research on DMG relative to autism may be in the

immunilogical area. Recent publications have shown that certain

components of the immune system may be abnormal in people with

autism. These include decreased number of helper T-cells and B-

cells, reduced natural killer cell activity, inhibition of

macrophage activity and increased interferon levels. DMG's ability

to modulate the immune system may work to correct a basic defect in

the immune system of autistic children that is responsible for the

symptoms being displayed. Further studies in this area are needed to

further elucidate the specific role DMG may play in reversing autism.

Inflammation and Autoimmune Conditions

Dimethylglycine (DMG) has been found to have an anti-inflammatory

property. This effect was found in a rat study done at Clemson

University by Dr. Lawson. DMG was found to prevent and reduce

an inflammatory response in a Rheumatoid Collagen II induced model

used in the rat. Under these conditions, 78% of the test rats given

DMG did not develop the characteristic inflammation and swelling as

seen in the control animals. This study shows that DMG, probably

through modulation of the immune system, can be helpful in treating

degenerative joint problems and rheumatoid arthritis where

inflammation is a major symptom. DMG has been used successfully to

aid in the reduction of pain, inflammation and joint tenderness in

individuals with rheumatoid arthritis. Recommended program calls for

using 250-375 mg of DMG taken 3 to 4 times daily. The effectiveness

of DMG as an anti-inflammatory nutrient works even better when

combined with the Green Lipped Mussel (Perna Canaliculus). An

excellent program for both rheumatoid and Osteo-arthritis would be

(6) 500 mg capsules of Perna and from 570-1,000 mg of DMG daily,

taken in divided doses.

Lupus Study

Systemic Lupus Erythematosis (SLE) or Lupus is considered to be the

prototypic systemic autoimmune disease. Symptoms for the disease

include skin rash, severe inflammation, joint pain and progressive

kidney failure. The common denominator among Lupus patients is

increased immunoglobulin IgG autoantibody to DNA.

At present, there is no really effective treatment method to treat

this autoimmune disease. An accepted mouse model to follow the

progression of the disease is the MRI-lpr mouse model. A study was

done to determine the effects of Perna (Green Lipped Mussel),

Dimethylglycine (DMG) and the combination of Perna and DMG together,

upon the progression of Lupus in these mice, over a 12-week period.

Areas evaluated included lymphocytic markers: CD4 (helper T-cells),

CD8+ (Cytotoxic T-cells), CD19, Cytokines (IL-4, IL-6, IL-10, Tumor

Nucrosis Factor – alpha) and antinuclear antibodies to both single

and double standard DNA. Although this is highly technical, it is

important to understand the basic tests that were done.

The results of the experiment were totally unexpected in that DMG

and Perna used by themselves made no significant differences

compared to the control group. However, when DMG and Perna were used

together, amazing results were achieved, including:

Significant reduction of the Cytotoxic T-cells (CD8+)

Significant reduction of the inflammatory Cytokines IL-6 and IL-10.

The role of IL-10 in the induction of autoantibodies in human SLE

has been recently suggested.

Increased levels of Tumor Nucrosis Factor alpha (TNF-alpha). Lupus

mice have exceptionally low levels of TNF-alpha and replacement

therapy with TNF-alpha significantly delays the development of

kidney damage.

Significant decreases in nuclear autoantibodies to both single and

double stranded autoantibodies. The decrease in lower serum levels

of IL-10 could be associated with this observation.

This experiment provided substantial evidence as to the synergistic

combination of Perna and DMG as a possible treatment protocol for

SLE. Further studies are now underway to substantiate whether this

combination would also be effective in treating human SLE (Lupus).

Initial results in this area have been very promising. A U.S. Patent

is now pending, based upon this remarkable discovery.

U.S. Patents

Due to the extensive research done on DMG over the past twenty

years, (5) U.S. Patents have been issued to FoodScience Corporation

for the discoveries surrounding Dimethylglycine:

US PATENT 4,631,189 " N, N-Dimethylglycine and Use in Immune

Response "

US PATENT 4,994,492 " Treatment of Melanoma Using N, N-

Dimethylglycine "

US PATENT 5,026,728 " Treatment of Arthritis Using N, N-

Dimethylglycine "

US PATENT 5,118,618 " Dimethylglycine Enhancement of Antibody

Production "

US PATENT 4,968,839 " Process for the Preparation of N, N-

Dimethylglycine (DMG) "

Conclusion

Dimethylglycine (DMG) is a versatile health potentiating and healing

nutrient that can be used to maintain good health, enhance

performance and productivity as well as aid the body in the healing

and restoration process. This Technical Bulletin has only touched

the surface of the many areas where DMG has been found to be

beneficial to the immune response, detoxification, mental and

physical performance, enhancement to cellular metabolism and to many

other specific areas as mentioned in this article. When DMG is

combined with other beneficial nutritional and therapeutic food

factors, such as Perna (Green Lipped Mussel) additional synergistic

effects may result. DMG can be safely combined with any nutritional

or therapeutic product without negative side effects.

Dimethylglycine is a time-tested, scientifically proven and

verifiable nutritional supplement that brings about greater

vitality, good health and cellular regeneration. Continued research

with this marvelous nutrient will reveal even more about how DMG can

be used to combat the stress and the health problems that affect

most people. Dimethylglycine is available in a 125 mg sublingual

tablet in Health Food Stores under the brand name, FoodScience

Aangamik™ DMG.

Alphabetical Listing of References

, A. " DMG Proving to be a Valuable Aid in Competition, " Horse

World, p. 20 Oct, 1982

Gannon, J. and Kendall, R., " A Clinical Evaluation of N, N-

Dimethylglycine (DMG) and Diisopropylammonium Dichloroacetate (DIPA)

on the Performance of Racing Greyhouds, Canine Practice, 9. 7 Nov-

Dec, 1982

Graber, G., Goust. J., Glassman, A., Kendall, R., and Loadholt,

C., " Immunomodulating Properties of Dimethylglycine in Humans " J Inf

Disease, 143, 101, 1981

Livine, S., Myhre, G., , G., Burns, J. " Effect of a Nutritional

Supplement Containing N, N-Dimethylglycine (DMG) on the Racing

Standardbred, Equine Practice, 4: March, 1982

MacKenzie, C., and Frisell, W., " Metabolism of Dimethylglycine by

Liver Mitochondria, " J Biol Chem., 232, 417, 1958

Meduski, J., Hyman, S., Kilz, R., Kim, K., Thein, P. And Yochimoto,

T., 1980 Pacific Slope Biochemical Conference. Abst. July 7-9, 1980,

U. Of California, San Diego

Roach, E., and Carlin, L., " N, N-Dimethylglycine for Epilepsy, " New

England Journal of Medicine, 307, 1081, 1982

Watanabe A., Treatment of chronic hepatitis using whey protein (non-

heated). Presented at 16th Intl. Congress on Nutrition, Montreal,

1997

Reap, E. and Lawson, J. " Stimulation of the Immune Response by

Dimethylglycine, a non-toxic metabolite " J. Laboratory and Clinical

Medicine, 115, 481 (1990)

Wang, C. and Lawson J. " The Effects on the Enhancement of Monoclonal

Antibody Production, " Annual Meeting of the American Soc. Of

Microbiology, Oct. 1988.

Reap, E. and Lawson, J. " The Effects of Dimethylglycine on B-16

Melanoma in Mice " , Annual Meeting of the American Soc. Of

Microbiology., Oct. 1988

, W., " Lactic Acid and DMG " , The Quarter Racing Journal, June

46, 1988.

Sellnow, L., " DMG, Properties and Properties " The Blood Horse 3855,

June 1987.

Mani, S., Whitesides J. and Lawson J. " Role of Dimethylglycine in

Melanoma Inhibition – Abstract from Nutrition and Cancer Prevention:

American Institute for Cancer Research " , Sept. 1999.

, M. " Some Nutri-Clinical Applications of N, N-

Dimethylglycine " Townsend Letter for Doctors, June 1988.

Return to Supplements page

*This information has not been evaluated by the Food and Drug

Administration. This product is not intended to diagnose, treat,

cure or prevent any disease.

You should always consult with your healthcare professional before

starting any supplementation program. Please view our full legal

disclaimer for more information.

Organix-South, Inc. brings you Organix's Natural Health.

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