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Re: What is PCR?

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Hi Dee and all, I just went to the gyn b/c I still have some bleeding from granulation following the hysterectomy last May. He is using silver nitrate to help it heal. He also discovered yeast. He did a culture and at my request looked under the scope and saw spores and yeast. So then I just double checked that he requested the yeast to be typed and sensitivity studies run on it. He said that this lab wouldn't do that. The lab simply used PCR to confirm the diagnosis of yeast. What good is that!!! He already saw it. I demanded that it be done since I had a whole year of trying to rid my body of rare resistant non-Albicans yeast. So then he did another culture and sent it off for typing and sensitivities to another lab. So my point is that we ladies should find out what is going to be determined from our cultures and make

sure we are getting the right information. I don't want to pay $100 dollars to just verify what he saw under the scope and have no idea if it is that old Krusei yeast that I battled so long ago and have no idea if a med will work. The routine one in this case would be done by PCR and add no value to my problem. Sandi

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I agree with you Sandi,

That's why I didn't think it was routinely done (the PRC test) and more so in the settings of laboratories or research and looking at DNA and other sequences of cellular biology, and was somewhat surprised when a member said she'd had it done. I hope she writes back to tell us what they found with doing that for her. ;) I've only ever seen it mentioned with all those studies & abstracts I read but not in common usage.

I'm glad your doctor did run a test or do a culture etc., to tell the 'type' of yeast that's presenting. That's different than a PCR test from my understanding and I hope it's not the Krusai one again.

Sorry to hear you're still having problems from the hysterectomy hon. Jeesh when will it all just quit and say 'Enough!' *groan* I just hope you're not in a lot of pain with it, and am so sorry.

Big hugs and wish it could be more. *sigh*

Dee~

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All I know is that my PCR was normal That's all they told me I just seem to have extremely fragile skin and I'm trying to get a prescription for the Estrace That sounds like it could help me KristyDeeTroll wrote: I agree with you Sandi, That's why I didn't think it was routinely done (the PRC test) and more so in the settings of laboratories or research and looking at DNA and other sequences of cellular biology, and was somewhat surprised when a member said she'd had it done. I hope she writes back to tell us what they found with doing that for her. ;) I've only ever seen it mentioned with all those studies &

abstracts I read but not in common usage. I'm glad your doctor did run a test or do a culture etc., to tell the 'type' of yeast that's presenting. That's different than a PCR test from my understanding and I hope it's not the Krusai one again. Sorry to hear you're still having problems from the hysterectomy hon. Jeesh when will it all just quit and say 'Enough!' *groan* I just hope you're not in a lot of pain with it, and am so sorry. Big hugs and wish it could be more. *sigh* Dee~

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HI Kristy, thanks. ;)

I too hope you can get a prescription for Estrace, it really is wonderful to help build up that vag. tissue. It's food and nourishment at a cellular level. You might try asking for samples of it too. ;) Not sure of your age but if you're younger it's more than likely a doctor will resist so you've got to make sure to tell them that you only want to use a small dab. 'topically' and not inserted as that's the first thing they think of (insertion).

Good luck hon

Dee~ ;)

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hi sandi--

i am curious how you determined that you had the krusei yeast...i suspect that i

have a

non-albicans yeast and don't know how/what tests i should be getting to figure

this out.

do they not usually look at the culture under the microscope? how did you get

your doctor

to do these tests?

julie

>

> Hi Dee and all,

>

> I just went to the gyn b/c I still have some bleeding from granulation

following the

hysterectomy last May. He is using silver nitrate to help it heal. He also

discovered yeast.

He did a culture and at my request looked under the scope and saw spores and

yeast. So

then I just double checked that he requested the yeast to be typed and

sensitivity studies

run on it. He said that this lab wouldn't do that. The lab simply used PCR to

confirm the

diagnosis of yeast. What good is that!!! He already saw it.

>

> I demanded that it be done since I had a whole year of trying to rid my

body of rare

resistant non-Albicans yeast. So then he did another culture and sent it off

for typing and

sensitivities to another lab.

>

> So my point is that we ladies should find out what is going to be determined

from our

cultures and make sure we are getting the right information. I don't want to

pay $100

dollars to just verify what he saw under the scope and have no idea if it is

that old Krusei

yeast that I battled so long ago and have no idea if a med will work. The

routine one in

this case would be done by PCR and add no value to my problem.

>

> Sandi

>

>

>

>

>

>

> ---------------------------------

> Yahoo! Mail

> Bring photos to life! New PhotoMail makes sharing a breeze.

>

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Hi , Ask your gyn to look under the microscope and ask for cultures with typing and sensitivities. I went to Dr. Horowitz, a yeast/vaginitis specialist in CT many years ago and then I did months of research contacting pharmaceutical companies to see which med would be best when DR. H's suggestions didn't work. First I had parapsilosis, then Krusei. The labs need to classify them. The problem is most gyns just culture and get a + or - reading back. That is STUPID, especially when treating complicated patients with histories of infections. Good luck. Love, Sandi

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Sandi,

I went through the same thing, I had a hystorectomy in

2000 in the hopes to get rid of the chronic pelvic

pain. I never got better. I also started having yeast

problems about a year later. You can now add hormone

problems with that. When I started to have the yeast

problems it was Glabrata. So it was very tuff on me.

Now 90% of the time it is Glabrata. I can tell the

differance between the glabrata and candidia with

using the discharge. The candidia is like a cottage

cheese thick and clumpy, and the glabrata is kind of

runny at first then goes to a light creamy texture.

Hope you find relief, Tami

--- Kristy wrote:

> All I know is that my PCR was normal That's all

> they told me I just seem to have extremely fragile

> skin and I'm trying to get a prescription for the

> Estrace That sounds like it could help me

>

> Kristy

>

> DeeTroll wrote:

> I agree with you Sandi,

>

> That's why I didn't think it was routinely done

> (the PRC test) and more so in the settings of

> laboratories or research and looking at DNA and

> other sequences of cellular biology, and was

> somewhat surprised when a member said she'd had it

> done. I hope she writes back to tell us what they

> found with doing that for her. ;) I've only ever

> seen it mentioned with all those studies & abstracts

> I read but not in common usage.

>

> I'm glad your doctor did run a test or do a

> culture etc., to tell the 'type' of yeast that's

> presenting. That's different than a PCR test from

> my understanding and I hope it's not the Krusai one

> again.

>

> Sorry to hear you're still having problems from

> the hysterectomy hon. Jeesh when will it all just

> quit and say 'Enough!' *groan* I just hope you're

> not in a lot of pain with it, and am so sorry.

>

> Big hugs and wish it could be more. *sigh*

> Dee~

>

>

>

> **IF REPLYING TO THIS POST, PLEASE REMOVE ORIGINAL

> POST, THANKS**

>

> Our HOME page is

> http://groups.yahoo.com/group/VulvarDisorders

> to search our archives, files, articles, etc.

>

> ***

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sandi,

but you eventually got rid of it?? at least that is encouraging...what

medication did you end

up taking?

do you mean look under the microscope after they've done the culture, is that

not

something they don't normally do?

best,

julie

>

> Hi ,

>

> Ask your gyn to look under the microscope and ask for cultures with typing

and

sensitivities. I went to Dr. Horowitz, a yeast/vaginitis specialist in CT many

years ago and

then I did months of research contacting pharmaceutical companies to see which

med

would be best when DR. H's suggestions didn't work. First I had parapsilosis,

then Krusei.

The labs need to classify them. The problem is most gyns just culture and get a

+ or -

reading back. That is STUPID, especially when treating complicated patients

with histories

of infections.

>

> Good luck.

> Love,

> Sandi

>

>

>

> ---------------------------------

> Yahoo! Mail

> Use Photomail to share photos without annoying attachments.

>

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