Re: Yeast supplements that don't taste bad?

[Guest guest]

Not sure if Candex is something you can consider--- it is very gentle  and is not associated with die offs.We use it regularly for maintenance.

 

I am mentoring someone with a 3 yr. old daughter that can sniff out supplements a mile away. She takes Nystatin fine, but is looking for a long term natural approach for yeast in between Nystatin doses. I know GSE would be out of the question. I was wondering what yeast supplements are easy to hide. Any suggestions?

[Guest guest]

You may want to research biofilm protocols by Dr Usman, Peta Cohen or Dr Klinghardt/Amy Derksen. Until you clear the gut of bacteria, viruses, heavy metals and other junk the yeast will continue to try. My son needs to stay on difllucan which requires a script and does not taste too bad. She could also try grapefruit seed extract as a natural alternative.

To: mb12 valtrex Sent: Mon, November 30, 2009 1:16:26 AMSubject: Yeast supplements that don't taste bad?

I am mentoring someone with a 3 yr. old daughter that can sniff out supplements a mile away. She takes Nystatin fine, but is looking for a long term natural approach for yeast in between Nystatin doses. I know GSE would be out of the question. I was wondering what yeast supplements are easy to hide. Any suggestions?

[Guest guest]

BrainChild Nutrtitionals sells some extracts that I like- Oregon Grape and Pau D Arco. Also Biocidin by BioBotanical Research is a super antifungal. Kirkman makes a Yeast-Aid powder that hides ok with juice. They all have a medicinal taste, but not bad. I find a preventative herbal schedule works better than Rx antifungals. There does not seem to be that rebound effect like you see when you discontinue Diflucan, Nystatin, etc. Of course, there is a time and place for Rx meds, but overall have had more success with herbals.

Subject: Yeast supplements that don't taste bad?To: mb12 valtrex Date: Monday, November 30, 2009, 6:16 AM

I am mentoring someone with a 3 yr. old daughter that can sniff out supplements a mile away. She takes Nystatin fine, but is looking for a long term natural approach for yeast in between Nystatin doses. I know GSE would be out of the question. I was wondering what yeast supplements are easy to hide. Any suggestions?

[Guest guest]

I've been hiding ACV in apple juice lately and it's working. I didn't think it

would.

>

> I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

>

[Guest guest]

If your child can swallow pills, ACV comes in capsule form,too.

> >

> > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> >

>

[Guest guest]

Does ACV combat yeast in our kids? I thought it would promote more yeast growth.

Has anyone tried this? Does it help?

Thanks

Angay

> > >

> > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > >

> >

>

[Guest guest]

I missed the beginning of this thread, but at work, we have a yeast program,

featuring natural antifungals that taste good. The ones we have that would be

fairly easy to hide include Pau D'Arco, Oregon Grape and Olive Leaf Extract, and

our Cat's Claw is antifungal, and could be used this way too. We also make

Lomatium and carry Biocidin, but both of these are stronger-tasting, not as easy

to hide. They are all alcohol-free and organic.

Terri from BrainChild

www . brainchildnutritionals . com

>

> I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

>

[Guest guest]

I missed the beginning of this thread, but at work, we have a yeast program,

featuring natural antifungals that taste good. The ones we have that would be

fairly easy to hide include Pau D'Arco, Oregon Grape and Olive Leaf Extract, and

our Cat's Claw is antifungal, and could be used this way too. We also make

Lomatium and carry Biocidin, but both of these are stronger-tasting, not as easy

to hide. They are all alcohol-free and organic.

Terri from BrainChild

www . brainchildnutritionals . com

>

> I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

>

[Guest guest]

I missed the beginning of this thread, but at work, we have a yeast program,

featuring natural antifungals that taste good. The ones we have that would be

fairly easy to hide include Pau D'Arco, Oregon Grape and Olive Leaf Extract, and

our Cat's Claw is antifungal, and could be used this way too. We also make

Lomatium and carry Biocidin, but both of these are stronger-tasting, not as easy

to hide. They are all alcohol-free and organic.

Terri from BrainChild

www . brainchildnutritionals . com

>

> I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

>

[Guest guest]

How much ACV do you use?

> >

> > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> >

>

[Guest guest]

Terri- We just started the Brainchild Protocol this week. I really like the idea

of rotating the antifungals to keep the candida " off guard " .

Do most people go the full 6 weeks?

Nanci

> >

> > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> >

>

[Guest guest]

Terri- We just started the Brainchild Protocol this week. I really like the idea

of rotating the antifungals to keep the candida " off guard " .

Do most people go the full 6 weeks?

Nanci

> >

> > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> >

>

[Guest guest]

Terri- We just started the Brainchild Protocol this week. I really like the idea

of rotating the antifungals to keep the candida " off guard " .

Do most people go the full 6 weeks?

Nanci

> >

> > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> >

>

[Guest guest]

Yes, I think most of us find that we have to go 6 weeks or more. It took me

about 2.5 months the first time to get our very bad yeast and clostridia

situation under control (according to testing). I actually continued rotating

the antifungals for quite awhile after that, because after our bad yeast

experiences, I really did not want it to come back. This was not hard to do and

seemed to be easy on my son.

In most of our kids, yeast will not be completely under control until you get

the bulk of the heavy metals out. Since the natural antifungals are easy on the

liver, and do not kill off all the good bacteria, you can do as much or as

little as you need to, long-term, to keep the bad stuff away. I slowly cut back

more and more, until I could stop completely, and then I rotated for a few more

days any time I saw any signs of yeast recurring.

Terri

-- In mb12 valtrex , " Nanci " wrote:

>

> Terri- We just started the Brainchild Protocol this week. I really like the

idea of rotating the antifungals to keep the candida " off guard " .

> Do most people go the full 6 weeks?

>

> Nanci

>

>

> > >

> > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > >

> >

>

[Guest guest]

I noticed that the protocol is keeps the same order of the antifungals in

rotation. I wonder if mixing up that order (ie OLE, oregon grape, lomatium, then

the next week oregon grape, lomatium, OLE) in consecutive weeks might be even

more effective?

What is the reason that you want to keep the same order? Couldn't the yeast be

killed sooner by mixing up that order?

Just a thought as we start our 2nd antiyeast for 4 days today.

Thanks in advance

Nanci

> > > >

> > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > >

> > >

> >

>

[Guest guest]

The reason we keep the same order is to have as long a time pass between periods

of using the same antifungal as possible, so the yeast doesn't get resistant to

it. When you use i.e. 4 days of pau d'arco, then 4 days oregon grape, then 4

days biocidin, then start over, you hit the yeast from 3 different directions,

and you don't give it long enough with any single one to become resistant.

We don't want a big die off reaction, so killing it quickly is not so much the

goal as getting it gone in a way that doesn't shock the child's body, and

keeping it away, without letting it build up a resistance to any single

antifungal. That's what the rotation does.

The yeast battle is not a quick skirmish, it is most often a protracted siege.

It's a matter of getting it under control then doing some lower level of

maintenance, so it stays that way. We don't want to lose the effectiveness of

our antifungal weapons, because then it all moves back in and you have to start

all over again.

Terri

> > > > >

> > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > > >

> > > >

> > >

> >

>

[Guest guest]

The reason we keep the same order is to have as long a time pass between periods

of using the same antifungal as possible, so the yeast doesn't get resistant to

it. When you use i.e. 4 days of pau d'arco, then 4 days oregon grape, then 4

days biocidin, then start over, you hit the yeast from 3 different directions,

and you don't give it long enough with any single one to become resistant.

We don't want a big die off reaction, so killing it quickly is not so much the

goal as getting it gone in a way that doesn't shock the child's body, and

keeping it away, without letting it build up a resistance to any single

antifungal. That's what the rotation does.

The yeast battle is not a quick skirmish, it is most often a protracted siege.

It's a matter of getting it under control then doing some lower level of

maintenance, so it stays that way. We don't want to lose the effectiveness of

our antifungal weapons, because then it all moves back in and you have to start

all over again.

Terri

> > > > >

> > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > > >

> > > >

> > >

> >

>

[Guest guest]

The reason we keep the same order is to have as long a time pass between periods

of using the same antifungal as possible, so the yeast doesn't get resistant to

it. When you use i.e. 4 days of pau d'arco, then 4 days oregon grape, then 4

days biocidin, then start over, you hit the yeast from 3 different directions,

and you don't give it long enough with any single one to become resistant.

We don't want a big die off reaction, so killing it quickly is not so much the

goal as getting it gone in a way that doesn't shock the child's body, and

keeping it away, without letting it build up a resistance to any single

antifungal. That's what the rotation does.

The yeast battle is not a quick skirmish, it is most often a protracted siege.

It's a matter of getting it under control then doing some lower level of

maintenance, so it stays that way. We don't want to lose the effectiveness of

our antifungal weapons, because then it all moves back in and you have to start

all over again.

Terri

> > > > >

> > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > > >

> > > >

> > >

> >

>

[Guest guest]

Makes sense! Thank you!

> > > > > >

> > > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > > > >

> > > > >

> > > >

> > >

> >

>

[Guest guest]

Makes sense! Thank you!

> > > > > >

> > > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > > > >

> > > > >

> > > >

> > >

> >

>

[Guest guest]

Has Brainchild ever collected info from parents about prescription antifungals

not working? The reason I ask is we just did Nystatin and it was a total waste

of money for us.

We had seen some gains with OLE in the past, but I was not rotating, so I'm

guessing the candida got resistant.

> > > > > >

> > > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > > > >

> > > > >

> > > >

> > >

> >

>

[Guest guest]

Has Brainchild ever collected info from parents about prescription antifungals

not working? The reason I ask is we just did Nystatin and it was a total waste

of money for us.

We had seen some gains with OLE in the past, but I was not rotating, so I'm

guessing the candida got resistant.

> > > > > >

> > > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > > > >

> > > > >

> > > >

> > >

> >

>

[Guest guest]

Has Brainchild ever collected info from parents about prescription antifungals

not working? The reason I ask is we just did Nystatin and it was a total waste

of money for us.

We had seen some gains with OLE in the past, but I was not rotating, so I'm

guessing the candida got resistant.

> > > > > >

> > > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > > > >

> > > > >

> > > >

> > >

> >

>

[Guest guest]

Antifolates as antimycotics? Connection between the folic acid cycle and the

ergosterol biosynthesis pathway in Candida albicans.

Navarro-Martínez MD, Cabezas-Herrera J, Rodríguez-López JN.

Grupo de Investigación de Enzimología, Departamento de Bioquímica y Biología

Molecular A, Facultad de Biología, Universidad de Murcia, E-30100 Espinardo,

Murcia, Spain.

The increased incidence of invasive mycoses and the emerging problem of

antifungal drug resistance have encouraged the search for new antifungal agents

or effective combinations of existing drugs. Infections due to Candida albicans

are usually treated with azole antifungals such as fluconazole, ketoconazole or

itraconazole. Whilst azoles may have little or no toxicity, they generally offer

rather poor fungicidal activity. Even in the absence of resistance, treatment

failures or recurrent infections are not uncommon, especially in

immunocompromised individuals. Here we demonstrate that the non-classical

antifolate pyrimethamine shows synergy with azole antifungal compounds and

interferes with the ergosterol biosynthesis pathway in C. albicans. By

disturbing folate metabolism in this fungus, pyrimethamine can inhibit

ergosterol production. The molecular connection between the folic acid cycle and

the ergosterol biosynthesis pathway is discussed and we show that the

filamentous form of this fungus is more susceptible to methotrexate than the

yeast form because the drug is more effectively transported through the membrane

of the filamentous form. When used to treat the hyphal form, methotrexate showed

synergy with other antifungals such as azoles and terbinafine. This finding

could have important clinical applications, as a combination of azoles with

antifolates and/or inhibitors of folic acid synthesis could represent an

attractive alternative for the treatment of C. albicans infections.

Publication Types:

Research Support, Non-U.S. Gov't

PMID: 17046206 [PubMed - indexed for MEDLINE

Growth inhibition of Candida albicans by folate pathway inhibitors. Their

potential in the selection of auxotrophs.

Henson OE, McClary DO.

Growth studies were conducted on C. albicans in a glucose - salts - biotin (GSB)

medium in the presence of folate inhibitors. Sulfanilamide inhibited growth

which was restored by PABA or tetrahydrofolate (THF). Aminopterin inhibited

growth to about the same level as did sulfanilamide, but this inhibition was not

reversed with PABA nor THF, singly or in combination. Inhibition by combined

sulfanilamide and aminopterin was synergistic, reducing growth by more than 90%

for 48 h. The sulfanilamide component of the combined inhibition was reversed by

PABA or THF to the level of that of aminopterin alone. Cytochrome synthesis was

not affected by the inhibitors, but marked increases occurred in

alpha-ketoglutarate, malate, isocitrate, and pyruvate dehydrogenases, especially

in the presence of both inhibitors. The pyrimidines in combination with

sulfanilamide were as inhibitory as was the combination of aminopterin and

sulfanilamide, but they had no effect when added alone or in combination with

aminopterin. Unlike the pyrimidines, the purines stimulated about a 50% recovery

from inhibition by either of the inhibitors. Growth inhibition by combined

sulfanilamide and aminopterin was overcome by about 50% by the addition of the

THF-mediated end-produits: deoxythymidylate, adenine, histidine and methionine.

The use of GSB medium containing adenine, histidine, methionine and the folate

inhibitors but without deoxythymidylate resulted in thymineless death of

prototrophic cells providing a method for the selection of auxotrophic mutants.

PMID: 386943 [PubMed - indexed for MEDLINE]

Folic acid and autism take another look

Anon DoR Nov.22-09

In 1991, CDC recommended folic acid supplementation for women who previously had

had an infant or fetus affected by an NTD and who planned to have more children

(1).

CDC recommended that these women take a 4-mg daily dose of folic acid (under a

physician's supervision) beginning at least 1 month before conception and

continuing throughout the first trimester of pregnancy. (2)However the data on

folic acids reduction in Spinal Bifida and NTD is not as impressive as one may

think. Advances in technologies and elected abortions may in fact be the true

decline in these disorders.

Similarly the numbers of autistic type disorder have increase with women's

increase in folic acid supplementation.

Peruvian women taking prenatal supplementation were found to have a lowered zinc

status.(3) Zinc is important for neuronal growth and immune function. Zinc is

also recognized as an anti-oxidant. Folic acid is capable of depleting zinc and

iron competes for zinc. This could possibly leave a child in a vulnerable state.

Improving maternal zinc status through prenatal supplementation may improve

fetal neurobehavioral development(4)

Boris and Goldblatt found that 89% of their autistic study population was

positive for at least one snip in the MTHFR.(5)

High amounts of folic acid may result in high dihydrofolate which in turn

inhibits MTHFR.(6)

MTHFR is found to be involved in one carbon metabolism. One carbon metabolism is

responsible for detoxification,. Amino acid synthesis, fatty acid synthesis,

energy production ,and oxidative phosphorylation to name a few. It has been

found that folic acid interferes with one carbon metabolism(7)

In addition, Fetal adaptations to a high-folate environment may interfere with

folate metabolism postnatally, with serious consequences for the epigenetic

regulation of gene expression.(8)

Once one carbon metabolism is inhibited, the child is vulnerable to metals,

bacteria, and virus.

In many autistic patients reduced natural killer cell activity has been

identified. Folic acid has been shown to reduce natural killer cell activity in

post menopausal women.(9) Lower estrogen amounts ,in post menopausal women has

been clearly defined .Autistic disorder is much higher among boys than girls.

In sprue ,another common variant in autistic disorder, it has been found that

neurologic lesions have occurred during folic acid therapy. In pernicious anemia

neurologic lesions have been observed in patients receiving folic acid therapy.

Moreover, folic acid may interfere with glutamic acid metabolism making the

brain more susceptible to glutamate toxicity. Furthermore, Folic acid may

interfere with the nutrition of the spinal cord.(10)

Many of these children have gut motility issues and high levels of folic acid

will serve to in fact feed undesirable inhabitants if the intestine.

It is well established that excess folic acid in low B-12 status results in

cognitive decline(11)

Similarly, the 2004 PDR Nurses Drug Handbook states that folic acid may cause

altered sleep patterns, difficulty in

concentration,irritability,overactivity,depression ,confusion,abdominal

distention, flatulence, and bronchial spasm in addition to, prolonged folic acid

therapy may also cause decreased vitamin B-12 levels.

Recent studies have also found that mothers whom in fact had taken folic acid

supplements had offspring who were at a higher risk for leukemia.

The dangers of folic acid supplementation in the unborn fetus and small child

must be examined.

1.CDC Use of folic acid prevention of spinal bifida and other

neuraldefects1983-1991MMRW1991:40:513-6

2.Folic acid to reduce the number of cases of spinal bifida and other neural

tube defects MMWR1992;41(no.RR-14):1-7

3. O'Brien KO, Zavaleta N, Caufield LE, Yang D, Abrams SA. Influence of prenatal

iron and zinc supplements on supplemental iron absorption, red blood cell iron

incorporation, and iron status in prenant Peruvian women. Am J Clin Nutr.

1999;69:509–15

4 Merialdi M; Caulfield LE; Zavaleta N; Figueroa A; DiPietro JA

Center for Human Nutrition, Department of International Health, The s

Hopkins School of Hygiene and Public Health, Baltimore, MD, USA.

AM J Obstet Gynecol.1999; 1809 (2 pt 1):483-90

5. http://www.nationalautismassociation.org/pdf/IOM-Bradstreet.pdf.

6.Is folate good for everyone?

,Kim,Refsim Americam Journal of clinical nuritionVol.87,no.3,517-533 march

2008

7. Sauer, B. Mason, Sang-Woon Choi too much folate: A risk for cancer

and cardiovascular disease? Current opinion in clinical nutrition and metabolic

care 2009,12:30-36

8. C Sugden - Nutrition research reviews, 2006 - Cambridge Univ Press

9.Aron M. Troen ,Breeana ,Bess Sorensen ,Mark H. Wener, Abbey ston,

Brent

Wood, Selhub, Anne McTiernan, Yutaka Yasui, Evrim Oral, D. Potter,

Cornelia M. Ulrich J. Nutr. 136:189-194, January 2006

10. Anonymous. A warning regarding the use of folic acidNEJM;237;713-5

11. Martha Savaria , F Jacques, Irwin H Rosenberg and Selhub

American Journal of Clinical Nutrition, Vol. 85, No. 1, 193-200, January 2007

> > > > > >

> > > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

> > > > > >

> > > > >

> > > >

> > >

> >

>

[Guest guest]

Antifolates as antimycotics? Connection between the folic acid cycle and the

ergosterol biosynthesis pathway in Candida albicans.

Navarro-Martínez MD, Cabezas-Herrera J, Rodríguez-López JN.

Grupo de Investigación de Enzimología, Departamento de Bioquímica y Biología

Molecular A, Facultad de Biología, Universidad de Murcia, E-30100 Espinardo,

Murcia, Spain.

The increased incidence of invasive mycoses and the emerging problem of

antifungal drug resistance have encouraged the search for new antifungal agents

or effective combinations of existing drugs. Infections due to Candida albicans

are usually treated with azole antifungals such as fluconazole, ketoconazole or

itraconazole. Whilst azoles may have little or no toxicity, they generally offer

rather poor fungicidal activity. Even in the absence of resistance, treatment

failures or recurrent infections are not uncommon, especially in

immunocompromised individuals. Here we demonstrate that the non-classical

antifolate pyrimethamine shows synergy with azole antifungal compounds and

interferes with the ergosterol biosynthesis pathway in C. albicans. By

disturbing folate metabolism in this fungus, pyrimethamine can inhibit

ergosterol production. The molecular connection between the folic acid cycle and

the ergosterol biosynthesis pathway is discussed and we show that the

filamentous form of this fungus is more susceptible to methotrexate than the

yeast form because the drug is more effectively transported through the membrane

of the filamentous form. When used to treat the hyphal form, methotrexate showed

synergy with other antifungals such as azoles and terbinafine. This finding

could have important clinical applications, as a combination of azoles with

antifolates and/or inhibitors of folic acid synthesis could represent an

attractive alternative for the treatment of C. albicans infections.

Publication Types:

Research Support, Non-U.S. Gov't

PMID: 17046206 [PubMed - indexed for MEDLINE

Growth inhibition of Candida albicans by folate pathway inhibitors. Their

potential in the selection of auxotrophs.

Henson OE, McClary DO.

Growth studies were conducted on C. albicans in a glucose - salts - biotin (GSB)

medium in the presence of folate inhibitors. Sulfanilamide inhibited growth

which was restored by PABA or tetrahydrofolate (THF). Aminopterin inhibited

growth to about the same level as did sulfanilamide, but this inhibition was not

reversed with PABA nor THF, singly or in combination. Inhibition by combined

sulfanilamide and aminopterin was synergistic, reducing growth by more than 90%

for 48 h. The sulfanilamide component of the combined inhibition was reversed by

PABA or THF to the level of that of aminopterin alone. Cytochrome synthesis was

not affected by the inhibitors, but marked increases occurred in

alpha-ketoglutarate, malate, isocitrate, and pyruvate dehydrogenases, especially

in the presence of both inhibitors. The pyrimidines in combination with

sulfanilamide were as inhibitory as was the combination of aminopterin and

sulfanilamide, but they had no effect when added alone or in combination with

aminopterin. Unlike the pyrimidines, the purines stimulated about a 50% recovery

from inhibition by either of the inhibitors. Growth inhibition by combined

sulfanilamide and aminopterin was overcome by about 50% by the addition of the

THF-mediated end-produits: deoxythymidylate, adenine, histidine and methionine.

The use of GSB medium containing adenine, histidine, methionine and the folate

inhibitors but without deoxythymidylate resulted in thymineless death of

prototrophic cells providing a method for the selection of auxotrophic mutants.

PMID: 386943 [PubMed - indexed for MEDLINE]

Folic acid and autism take another look

Anon DoR Nov.22-09

In 1991, CDC recommended folic acid supplementation for women who previously had

had an infant or fetus affected by an NTD and who planned to have more children

(1).

CDC recommended that these women take a 4-mg daily dose of folic acid (under a

physician's supervision) beginning at least 1 month before conception and

continuing throughout the first trimester of pregnancy. (2)However the data on

folic acids reduction in Spinal Bifida and NTD is not as impressive as one may

think. Advances in technologies and elected abortions may in fact be the true

decline in these disorders.

Similarly the numbers of autistic type disorder have increase with women's

increase in folic acid supplementation.

Peruvian women taking prenatal supplementation were found to have a lowered zinc

status.(3) Zinc is important for neuronal growth and immune function. Zinc is

also recognized as an anti-oxidant. Folic acid is capable of depleting zinc and

iron competes for zinc. This could possibly leave a child in a vulnerable state.

Improving maternal zinc status through prenatal supplementation may improve

fetal neurobehavioral development(4)

Boris and Goldblatt found that 89% of their autistic study population was

positive for at least one snip in the MTHFR.(5)

High amounts of folic acid may result in high dihydrofolate which in turn

inhibits MTHFR.(6)

MTHFR is found to be involved in one carbon metabolism. One carbon metabolism is

responsible for detoxification,. Amino acid synthesis, fatty acid synthesis,

energy production ,and oxidative phosphorylation to name a few. It has been

found that folic acid interferes with one carbon metabolism(7)

In addition, Fetal adaptations to a high-folate environment may interfere with

folate metabolism postnatally, with serious consequences for the epigenetic

regulation of gene expression.(8)

Once one carbon metabolism is inhibited, the child is vulnerable to metals,

bacteria, and virus.

In many autistic patients reduced natural killer cell activity has been

identified. Folic acid has been shown to reduce natural killer cell activity in

post menopausal women.(9) Lower estrogen amounts ,in post menopausal women has

been clearly defined .Autistic disorder is much higher among boys than girls.

In sprue ,another common variant in autistic disorder, it has been found that

neurologic lesions have occurred during folic acid therapy. In pernicious anemia

neurologic lesions have been observed in patients receiving folic acid therapy.

Moreover, folic acid may interfere with glutamic acid metabolism making the

brain more susceptible to glutamate toxicity. Furthermore, Folic acid may

interfere with the nutrition of the spinal cord.(10)

Many of these children have gut motility issues and high levels of folic acid

will serve to in fact feed undesirable inhabitants if the intestine.

It is well established that excess folic acid in low B-12 status results in

cognitive decline(11)

Similarly, the 2004 PDR Nurses Drug Handbook states that folic acid may cause

altered sleep patterns, difficulty in

concentration,irritability,overactivity,depression ,confusion,abdominal

distention, flatulence, and bronchial spasm in addition to, prolonged folic acid

therapy may also cause decreased vitamin B-12 levels.

Recent studies have also found that mothers whom in fact had taken folic acid

supplements had offspring who were at a higher risk for leukemia.

The dangers of folic acid supplementation in the unborn fetus and small child

must be examined.

1.CDC Use of folic acid prevention of spinal bifida and other

neuraldefects1983-1991MMRW1991:40:513-6

2.Folic acid to reduce the number of cases of spinal bifida and other neural

tube defects MMWR1992;41(no.RR-14):1-7

3. O'Brien KO, Zavaleta N, Caufield LE, Yang D, Abrams SA. Influence of prenatal

iron and zinc supplements on supplemental iron absorption, red blood cell iron

incorporation, and iron status in prenant Peruvian women. Am J Clin Nutr.

1999;69:509–15

4 Merialdi M; Caulfield LE; Zavaleta N; Figueroa A; DiPietro JA

Center for Human Nutrition, Department of International Health, The s

Hopkins School of Hygiene and Public Health, Baltimore, MD, USA.

AM J Obstet Gynecol.1999; 1809 (2 pt 1):483-90

5. http://www.nationalautismassociation.org/pdf/IOM-Bradstreet.pdf.

6.Is folate good for everyone?

,Kim,Refsim Americam Journal of clinical nuritionVol.87,no.3,517-533 march

2008

7. Sauer, B. Mason, Sang-Woon Choi too much folate: A risk for cancer

and cardiovascular disease? Current opinion in clinical nutrition and metabolic

care 2009,12:30-36

8. C Sugden - Nutrition research reviews, 2006 - Cambridge Univ Press

9.Aron M. Troen ,Breeana ,Bess Sorensen ,Mark H. Wener, Abbey ston,

Brent

Wood, Selhub, Anne McTiernan, Yutaka Yasui, Evrim Oral, D. Potter,

Cornelia M. Ulrich J. Nutr. 136:189-194, January 2006

10. Anonymous. A warning regarding the use of folic acidNEJM;237;713-5

11. Martha Savaria , F Jacques, Irwin H Rosenberg and Selhub

American Journal of Clinical Nutrition, Vol. 85, No. 1, 193-200, January 2007

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> > > > > > I am mentoring someone with a 3 yr. old daughter that can sniff out

supplements a mile away. She takes Nystatin fine, but is looking for a long term

natural approach for yeast in between Nystatin doses. I know GSE would be out of

the question. I was wondering what yeast supplements are easy to hide. Any

suggestions?

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