Guest guest Posted August 26, 2004 Report Share Posted August 26, 2004 Hi ; Thanks for your kind words. When I'm not researching PSC and IBD, I'm researching plant metabolism. I like to take time to " smell the roses " [or in this case the Petunias!]: Plant Physiol. 2004 Aug;135(4):1993-2011. Understanding in vivo benzenoid metabolism in petunia petal tissue. Boatright J, Negre F, Chen X, Kish CM, Wood B, Peel G, Orlova I, Gang D, D, Dudareva N. Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907. In vivo stable isotope labeling and computer-assisted metabolic flux analysis were used to investigate the metabolic pathways in petunia (Petunia hybrida) cv leading from Phe to benzenoid compounds, a process that requires the shortening of the side chain by a C(2) unit. Deuterium-labeled Phe ((2)H(5)-Phe) was supplied to excised petunia petals. The intracellular pools of benzenoid/phenylpropanoid-related compounds (intermediates and end products) as well as volatile end products within the floral bouquet were analyzed for pool sizes and labeling kinetics by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. Modeling of the benzenoid network revealed that both the CoA-dependent, beta-oxidative and CoA-independent, non-beta- oxidative pathways contribute to the formation of benzenoid compounds in petunia flowers. The flux through the CoA-independent, non-beta- oxidative pathway with benzaldehyde as a key intermediate was estimated to be about 2 times higher than the flux through the CoA- dependent, beta-oxidative pathway. Modeling of (2)H(5)-Phe labeling data predicted that in addition to benzaldehyde, benzylbenzoate is an intermediate between l-Phe and benzoic acid. Benzylbenzoate is the result of benzoylation of benzyl alcohol, for which activity was detected in petunia petals. A cDNA encoding a benzoyl-CoA:benzyl alcohol/phenylethanol benzoyltransferase was isolated from petunia cv using a functional genomic approach. Biochemical characterization of a purified recombinant benzoyl-CoA:benzyl alcohol/phenylethanol benzoyltransferase protein showed that it can produce benzylbenzoate and phenylethyl benzoate, both present in petunia corollas, with similar catalytic efficiencies. PMID: 15286288 Best regards, Dave R. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted August 26, 2004 Report Share Posted August 26, 2004 Like I totally understand this! Ha Ha Lee On Thursday, August 26, 2004, at 01:37 PM, wrote: > > Hi ; > > Thanks for your kind words. When I'm not researching PSC and IBD, I'm > researching plant metabolism. I like to take time to " smell the > roses " [or in this case the Petunias!]: > > Plant Physiol. 2004 Aug;135(4):1993-2011. > > Understanding in vivo benzenoid metabolism in petunia petal tissue. > > Boatright J, Negre F, Chen X, Kish CM, Wood B, Peel G, Orlova I, Gang > D, D, Dudareva N. > > Department of Horticulture and Landscape Architecture, Purdue > University, West Lafayette, Indiana 47907. > > In vivo stable isotope labeling and computer-assisted metabolic flux > analysis were used to investigate the metabolic pathways in petunia > (Petunia hybrida) cv leading from Phe to benzenoid > compounds, a process that requires the shortening of the side chain > by a C(2) unit. Deuterium-labeled Phe ((2)H(5)-Phe) was supplied to > excised petunia petals. The intracellular pools of > benzenoid/phenylpropanoid-related compounds (intermediates and end > products) as well as volatile end products within the floral bouquet > were analyzed for pool sizes and labeling kinetics by gas > chromatography-mass spectrometry and liquid chromatography-mass > spectrometry. Modeling of the benzenoid network revealed that both > the CoA-dependent, beta-oxidative and CoA-independent, non-beta- > oxidative pathways contribute to the formation of benzenoid compounds > in petunia flowers. The flux through the CoA-independent, non-beta- > oxidative pathway with benzaldehyde as a key intermediate was > estimated to be about 2 times higher than the flux through the CoA- > dependent, beta-oxidative pathway. Modeling of (2)H(5)-Phe labeling > data predicted that in addition to benzaldehyde, benzylbenzoate is an > intermediate between l-Phe and benzoic acid. Benzylbenzoate is the > result of benzoylation of benzyl alcohol, for which activity was > detected in petunia petals. A cDNA encoding a benzoyl-CoA:benzyl > alcohol/phenylethanol benzoyltransferase was isolated from petunia cv > using a functional genomic approach. Biochemical > characterization of a purified recombinant benzoyl-CoA:benzyl > alcohol/phenylethanol benzoyltransferase protein showed that it can > produce benzylbenzoate and phenylethyl benzoate, both present in > petunia corollas, with similar catalytic efficiencies. > > PMID: 15286288 > > Best regards, > > Dave R. > > > > > > > > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted August 27, 2004 Report Share Posted August 27, 2004 Well, now I have to ask! Someone once referred to you (Dave) as Professor. I have always wondered what that reference was about. I also am wondering if there are any medical doctors or other professionals on here who are posting to this list. Please do not take this as I am seeking someone with the letters behind their names and I am not valuing the information from everyone else - it is NOT that at all. Just curiosity I suppose. LINDA(Mom, Suzanne - 15, dx UC 1/04, PSC 3/04) Re: (OT) Hi ;Thanks for your kind words. When I'm not researching PSC and IBD, I'm researching plant metabolism. I like to take time to "smell the roses" [or in this case the Petunias!]:Plant Physiol. 2004 Aug;135(4):1993-2011. Understanding in vivo benzenoid metabolism in petunia petal tissue.Boatright J, Negre F, Chen X, Kish CM, Wood B, Peel G, Orlova I, Gang D, D, Dudareva N.Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907.In vivo stable isotope labeling and computer-assisted metabolic flux analysis were used to investigate the metabolic pathways in petunia (Petunia hybrida) cv leading from Phe to benzenoid compounds, a process that requires the shortening of the side chain by a C(2) unit. Deuterium-labeled Phe ((2)H(5)-Phe) was supplied to excised petunia petals. The intracellular pools of benzenoid/phenylpropanoid-related compounds (intermediates and end products) as well as volatile end products within the floral bouquet were analyzed for pool sizes and labeling kinetics by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. Modeling of the benzenoid network revealed that both the CoA-dependent, beta-oxidative and CoA-independent, non-beta-oxidative pathways contribute to the formation of benzenoid compounds in petunia flowers. The flux through the CoA-independent, non-beta-oxidative pathway with benzaldehyde as a key intermediate was estimated to be about 2 times higher than the flux through the CoA-dependent, beta-oxidative pathway. Modeling of (2)H(5)-Phe labeling data predicted that in addition to benzaldehyde, benzylbenzoate is an intermediate between l-Phe and benzoic acid. Benzylbenzoate is the result of benzoylation of benzyl alcohol, for which activity was detected in petunia petals. A cDNA encoding a benzoyl-CoA:benzyl alcohol/phenylethanol benzoyltransferase was isolated from petunia cv using a functional genomic approach. Biochemical characterization of a purified recombinant benzoyl-CoA:benzyl alcohol/phenylethanol benzoyltransferase protein showed that it can produce benzylbenzoate and phenylethyl benzoate, both present in petunia corollas, with similar catalytic efficiencies.PMID: 15286288Best regards,Dave R. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted August 27, 2004 Report Share Posted August 27, 2004 > Well, now I have to ask! > Someone once referred to you (Dave) as Professor. I have always wondered what that reference was about .... Hi , Yes, I am professor in the Horticulture Department at Purdue University; I teach courses on Vegetable Crops, Metabolic Plant Physiology, and Grants and Grantsmanship. Our Department web site is: http://www.hort.purdue.edu/hort/ I do research on plant biochemistry. Although I don't have any training in medicine, I think my background in biochemistry and research might be an asset to the group occassionally? But like everyone else in the group I am trying to understand PSC and IBD ... this was foreign to me just 12 months ago. And like other PSC care- givers here I'm struggling to come to grips with the future that this may hold for my son. Best regards, Dave (father of (19); PSC 07/03; UC 08/03) Quote Link to comment Share on other sites More sharing options...
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