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Re: Re: Is QPCR Better

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, I assume that the arrangement for letting you printing IESO standard (1st edition) at the end of your book is "no string attached". Who approved the copyright permission anyway? Wei Tang QLabgary rosen wrote: I have no financial interest

in Aerotech or other lab. Different labs have different products, services and abilities. I think it is useful to know about which lab one is talking about when discussing such services. Rosen Re: Re: Is QPCR Better , "Did" (pre-unification era) IESO only approve supply sold by Aerotech? Wei Tang QLab "Wane A. Baker" <wab@michaelsenginee ring.com> wrote: :1. I was uncertain how to interpret your reference to "...not less thana minimum of 100 spore equivalents. .." Is this the same as saying "morethan 100 SEs"?in the qPCR samples that we've submitted, the method

detection limit(MDL) was largely a factor of the particular standard used with anindividual run of a given species' primer and probe. but in most cases,the MDL was low single-digit spore equivalents (generally <3 SEs), withjust a few as high as 30 or 40 SEs.I wouldn't consider using qPCR if the MDL (or LOQ) was more than 100SEs. we choose to use aPCR when its high specificity and sensitivityare of paramount importance.2. is the "...IESO approved carpet dust collection technique... " thatwhich is described in one of the IESO standards?TIA for the clarifications.Wane> Carl,>> Please go to the Aerotech P & K web site that describes the ERMItechnology. I am sure other companies may offer similar programs but Iam familiar with the Aerotech offerings. Because QPCR needs plenty ofmaterial, you collect carpet dust using (an IESO approved) carpet dustcollection technique. I would assume that you could also take airsamples but the marketing release says dust.I have submitted airsamples, dust samples, and bulk samples for QPCR. When you take airsamples for QPCR you need to run the pump for several hours since youneed plenty of material.>

QLab5 DriveCherry Hill , NJ 08003www.QLabUSA. com Everyone is raving about the all-new Yahoo! Mail beta. QLab5 DriveCherry Hill , NJ 08003www.QLabUSA. com __________________________________________________

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Please forgive me if this seems off topic, but I actually don't think it is..There are some interesting parallels between this debate and the debate in the Internet engineering community about the use and promotion of open standards and interoperability for document and IP protocols. Maybe some of you might be able to gain some insights into the solving of your industry's problems from reading about the issues encountered elsewhere via-a-vis open standards versus standards that use proprietary software, patents, hardware, information or whatever..

Places to look as far as seeing some of these debates are many, but what comes to my mind are the IETF.org site, the CreativeCommons.org and ScienceCommons.org, eff.org,

gnu.org, etc. sites.. and others like them.. Searches on the above phrases might also bring up some useful stuff.. This isn't about IAQ, its about the various kinds of information sharing models out there and the relative merits of them all in different situations..

Again, if this seems off topic, please try to see it in a greater context.. This kind of debate is happening in all different kinds of disciplines and industries and I would think that your industry is no exception..

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Feitveit gave me permission because I asked. I don't pay them anything and they don't pay me anything.

Rosen

Re: Re: Is QPCR Better

,

"Did" (pre-unification era) IESO only approve supply sold by Aerotech?

Wei Tang

QLab

"Wane A. Baker" <wab@michaelsenginee ring.com> wrote:

:1. I was uncertain how to interpret your reference to "...not less thana minimum of 100 spore equivalents. .." Is this the same as saying "morethan 100 SEs"?in the qPCR samples that we've submitted, the method detection limit(MDL) was largely a factor of the particular standard used with anindividual run of a given species' primer and probe. but in most cases,the MDL was low single-digit spore equivalents (generally <3 SEs), withjust a few as high as 30 or 40 SEs.I wouldn't consider using qPCR if the MDL (or LOQ) was more than 100SEs. we choose to use aPCR when its high specificity and sensitivityare of paramount importance.2. is the "...IESO approved carpet dust collection technique... " thatwhich is described in one of the IESO standards?TIA for the clarifications.Wane> Carl,>> Please go to the Aerotech P & K web site that describes the ERMItechnology. I am sure other companies may offer similar programs but Iam familiar with the Aerotech offerings. Because QPCR needs plenty ofmaterial, you collect carpet dust using (an IESO approved) carpet dustcollection technique. I would assume that you could also take airsamples but the marketing release says dust.I have submitted airsamples, dust

samples, and bulk samples for QPCR. When you take airsamples for QPCR you need to run the pump for several hours since youneed plenty of material.>

QLab5 DriveCherry Hill , NJ 08003www.QLabUSA. com

Everyone is raving about the all-new Yahoo! Mail beta.

QLab5 DriveCherry Hill , NJ 08003www.QLabUSA. com

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