[CFSRES-UPD] CFS: Preliminary evidence of mitochondrial dysfunction associated w

[Guest guest]

It is known that at least a subgroup of CFS patients got ill with glandular

fever caused by EBV or CMV and never recovered and later got a diagnosis of

CFS.

This article is not on CFS but describes chronic EBV infection.

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BMC Infectious Diseases 2006, 6:15 doi:10.1186/1471-2334-6-15

Research article

Preliminary evidence of mitochondrial dysfunction associated with

post-infective fatigue after acute infection with Epstein Barr Virus

Suzanne D Vernon , Toni Whistler , Barbara Cameron , Ian B Hickie ,

C Reeves and Lloyd

Published 31 January 2006

C 2006 Vernon et al., licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative

Commons Attribution License (http://creativecommons.org/licenses/by/2.0),

which permits unrestricted use, distribution, and reproduction in any

medium, provided the original work is properly cited.

Abstract (provisional)

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Background

Acute infectious diseases are typically accompanied by non-specific symptoms

including fever, malaise, irritability and somnolence that usually resolve

on recovery. However, in some individuals these symptoms persist in what is

commonly termed post-infective fatigue. The objective of this pilot study

was to determine the gene expression correlates of post-infective fatigue

following acute Epstein Barr virus (EBV) infection.

Methods

We followed 5 people with acute mononucleosis who developed post-infective

fatigue of more than 6 months duration and 5 HLA-matched control subjects

who recovered within 3 months. Subjects had peripheral blood mononuclear

cell (PBMC) samples collected at varying time points including at diagnosis,

then every 2 weeks for 3 months, then every 3 months for a year. Total RNA

was extracted from the PBMC samples and hybridized to microarrays spotted

with 3,800 oligonucleotides.

Results

Those who developed post-infective fatigue had gene expression profiles

indicative of an altered host response during acute mononucleosis compared

to those who recovered uneventfully. Several genes including ISG20

(interferon stimulated gene), DNAJB2 (DnaJ [Hsp40] homolog and CD99), CDK8

(cyclin-dependent kinase 8), E2F2 (E2F transcription factor 2), CDK8

(cyclin-dependent kinase 8), and ACTN2 (actinin, alpha 2), known to be

regulated during EBV infection, were differentially expressed in

post-infective fatigue cases. Several of the differentially expressed genes

affect mitochondrial functions including fatty acid metabolism and the cell

cycle.

Conclusions

These preliminary data provide insights into alterations in gene transcripts

associated with the varied clinical outcomes from acute infectious

mononucleosis.

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Full-Text Article at:

http://www.cfsresearch.org/cfs/research/viruses/11.htm