HBOT & supplements & oxidative stress

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Br J Nutr. 2007 May 3;:1-8 [Epub ahead of print]

Effect of hyperbaric oxygen and vitamin C and E supplementation on

biomarkers of oxidative stress in healthy men.

Bader N, Bosy-Westphal A, Koch A, Rimbach G, Weimann A, Poulsen HE, Müller

MJ.

Insititute of Biological Chemistry and Nutrition, University of Hohenheim,

Garbenstrasse 28, D-70593, Stuttgart, Germany.

The objectives of the present study were to evaluate the effect of

normobaric and hyperbaric O2 (HBO) on plasma antioxidants and biomarkers

of oxidative stress in plasma and urine and to investigate the effect of a

4-week vitamin C plus E supplementation on HBO-induced oxidative stress.

Nineteen healthy men were exposed to HBO (100 % O2; 240 kPa) before and

after 4 weeks' supplementation with 500 mg vitamin C plus 165 mg

alpha-tocopherol equivalents. Exposure to 21 % O2 at 100 kPa served as

intra-individual controls (control). Samples for the analysis of plasma

antioxidants and oxidative stress biomarkers were collected before and

immediately after each treatment.

The present results showed that when compared with 'control', a single

exposure to HBO resulted in a decrease of plasma vitamin C (P = 0.027) and

an increase of lipid peroxides (P = 0.0008) and urinary

8-oxo-deoxyguanosine (8-oxodG) excretion (P = 0.006). Oxidative stress was

not prevented by a 4-week supplementation with vitamins C and E.

HBO-induced changes in plasma parameters correlated with basal antioxidant

levels. The increase of urinary 8-oxodG after HBO plus supplementation

correlated negatively with vitamin E intake (P = 0.023).

We concluded that in healthy men HBO caused oxidative stress, which could

not be prevented by dietary vitamin C plus E supplementation. The present

data support the idea that HBO is a suitable model for oxidative stress in

healthy volunteers.

PMID: 17475085