Re: Digest Number 3107

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Thank you so much for your time in posting these excellent references,

!

Best,

a

On Fri, Oct 15, 2010 at 5:04 AM, <medical_translation >wrote:

> Medical Translation

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> Messages In This Digest (1 Message)

> 1a. Re: En>Es " Thrombin Generation Assay (TGA) " <#12baeeb608b0f03f_1a>From:

cgtradmed

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> 1a. Re: En>Es " Thrombin Generation Assay (TGA) "

>

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> by: " cgtradmed " cgtradmed@...

>

<cgtradmed@...?Subject=+Re%3A%20En%3EEs%20%22Thrombin%20Generation%20Assay%2\

0%28TGA%29%22> cgtradmed

> <http://profiles.yahoo.com/cgtradmed> Thu Oct 14, 2010 2:17 am (PDT)

>

>

> Hi a,

>

> These are 2 different tests !

>

> Here, there is a complete explanation /description (at least, the

> begining of the article) which states the differences. These differences

> are so dramatic that they justify the usefulness of both distinct tests :

>

> In Thrombosis Journal :

>

> Towards a standardization of thrombin generation assessment: The

> influence of tissue factor, platelets and phospholipids concentration

> on the normal values of Thrombogram-Thrombinoscope assay

>

> "

>

> Background

>

> The global clotting times (i.e. prothrombin time and partial

> thromboplastin time) are routinely used for the evaluation of human

> coagulation but they ignore the procedure of thrombin generation since

> at the time of clot formation only 3% of prothrombin is activated 1

> <http://www.thrombosisjournal.com/content/3/1/16#B1>,2

> <http://www.thrombosisjournal.com/content/3/1/16#B2>]. This is a

> constant observation either in normal plasma or in the presence of

> antithrombotic agents (i.e. enoxaparin or fondaparinux) [3

> <http://www.thrombosisjournal.com/content/3/1/16#B3>]. The global

> clotting times are mandatory for the detection of clotting factor

> deficiencies and the biological monitoring of treatment with coumarins

> and unfractionated heparin but they have a very limited value in the

> detection of hypercoagulable states [1

> <http://www.thrombosisjournal.com/content/3/1/16#B1>,2

> <http://www.thrombosisjournal.com/content/3/1/16#B2>]. Moreover, they

> are not significantly influenced by the presence of therapeutic

> concentrations of LMWHs or the indirect FXa inhibitors such as

> fondaparinux [3 <http://www.thrombosisjournal.com/content/3/1/16#B3>].

> Measuring of anti-Xa activity in plasma from patients treated with LMWHs

> has a limited predictive value for the clinical outcome [4

> <http://www.thrombosisjournal.com/content/3/1/16#B4>] and in some

> patients' populations (i.e elderly or obese patients) the anti-Xa levels

> in plasma are poorly correlated with the administered dose of LMWH 5

> <http://www.thrombosisjournal.com/content/3/1/16#B5>,6

> <http://www.thrombosisjournal.com/content/3/1/16#B6>].

>

> The study of thrombin generation performed either with clotting based

> assays or with chromogenic substrates is an old and established tool in

> blood coagulation research [7

> <http://www.thrombosisjournal.com/content/3/1/16#B7>-12

> <http://www.thrombosisjournal.com/content/3/1/16#B12>]. It describes all

> the phases of thrombin generation process (initiation, amplification and

> inhibition of thrombin generation as well as the integral amount of

> generated thrombin). According to the experimental system used, thrombin

> generation may be influenced by most of the factors playing a role in

> blood coagulation. However, thrombin generation assessment used to be a

> laborious and time-consuming method. The most sophisticated version of

> thrombin generation assay using a chromogenic substrate developed by

> Hemker's group was less time-demanding than the previous one, since it

> was fully automated accompanied with a software for the calculation of

> the area under thrombin generation curve (endogenous thrombin potential)

> [13 <http://www.thrombosisjournal.com/content/3/1/16#B13>]. The most

> important experimental limitation of this assay was that it could be

> done only in defibrinated platelet poor plasma (PPP). Thus at least two

> important components of blood coagulation (i.e. platelets and

> fibrinogen/fibrin) were absent [14

> <http://www.thrombosisjournal.com/content/3/1/16#B14>].

>

> The Thrombogram-Thrombinoscope assay is a step forward to the study of

> the blood coagulation process because, using a fluorogenic substrate, it

> allows obtaining thrombin generation curves in non-defibrinated platelet

> rich plasma (PRP) in a fully automated manner [15

> <http://www.thrombosisjournal.com/content/3/1/16#B15>]. An increasing

> body of evidence shows that Thrombogram-Thrombinoscope assay may be a

> useful tool in the diagnosis of acquired or congenital hypercoagulable

> states and it is sensitive enough, in patients with hemorrhagic

> diathesis [16, reviewed in 17]. Thrombogram is also sensitive to the

> presence of antiplatelet and antithrombotic agents [3

> <http://www.thrombosisjournal.com/content/3/1/16#B3>,18

> <http://www.thrombosisjournal.com/content/3/1/16#B18>]. "

>

> On : http://www.thrombosisjournal.com/content/3/1/16

>

> HTH

>

>

> Le 13/10/2010 22:03, gabrielamejias@... <gabrielamejias%40gmail.com>a

écrit :

> > Hi Colleagues!

> >

> > Could anybody tell me if Thrombin Generation Assay (TGA) and Thrombin

> Time

> > (TT) are the same thing?

> >

> > In a translation I'm editing, I've found TGA translated as 'Tiempo de

> > trombina', and not as 'Prueba de generamiento de trombina'.

> >

> > Thank you in advance!

> >

> > Regards from Buenos Aires,

> >

> > a

> >

> >

> >

> >

>

>