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>I was doing a search on Yahoo and found the following link on this " natural "

preservative:

>http://www.biochemresearch.com/citricidal%20as%20Cosmetic%20Preserve.htm

>Has any heard of this?

I have

>Has anyone used it/using it?

I am not using Citricidal

>Please let me know your experience & /or knowledge of this product.

The webpage you provided did not say that Citricidal was an effective

preservative. Many ingredients can be used as preservatives in

cosmetics. But there are only a few ingredients that have demonstrated

their effectiveness by reasonable challenge tests including thousands

of challenge tests run by cosmetic companies.

I have never seen any challenge tests for Citricidal. If Citricidal

were an effective cosmetic preservative, I would think that someone

would publish the results of a reasonable challenge test. I haven't

seen any.

from the message archives:

--------------------

>

>

>>Actually, they do---Citricidal (grapefruit seed extract). They do say they

>>won't use any synthetic preservatives unless requested and then no more than

>>1%.

>

>Citricidal may come from a natural source, Grapefruits, but according

>to the one Citricidal website:

>http://www.gfex.com/citricidal.htm

>

> " Citricidal® is synthesized from the polyphenolic compounds found in

>grapefruit seed and pulp. "

>

>The method of manufacturing Citricidal is described on

>http://www.nutriteam.com/gsewhat.html

>

>1) Grapefruit pulp and seeds (the by-product of expeller-extracted

>grapefruit juice) is dried and ground into a fine powder.

>

>2) The grapefruit powder is dissolved in purified water and distilled

>to remove the fiber and pectin.

>

>3) This distilled slurry is spray dried at low temperatures forming a

>concentrated grapefruit bioflavonoid powder.

>

>4) This concentrated powder is dissolved in vegetable glycerine and

>heated. Food grade ammonium chloride and ascorbic acid are added, and

>this mixture is heated under pressure.

>

>5) This material undergoes catalytic conversion using natural catalysts

>(including hydrochloric acid and natural enzymes.).

>

>6) This slurry is cooled, filtered and treated with ultraviolet light.

>

>and from http://www.nutriteam.com/gsewhat.html, referring to how

>Citricidal works:

>

> " It should come as no surprise that we know a lot more about what GSE

>is good for, than exactly how it works. "

>

>If Citricidal was a truely effective preservative, you would think that

>the manufacturer would publish preservative efficacy test on their

>website and major cosmetic companies would be using Citricidal in their

>formulas.

>

>I do not question the toxicity of Citricidal just it's effectiveness as

>a cosmetic preservative.

--------------------

>If Grape Seed Extract were an effective preservative, all the major

>cosmetic companies would be using GSE in their formulas. Aveda is the

>only large personal care company that lists GSE on their ingredient

>declaration for some products. But, in those Aveda products that use

>GSE, Aveda also uses olidinyl Urea, Methylparaben and Propylparaben

>(probably Germaben II). Why? Because GSE is NOT an effective

>preservative. It's that simple.

>

>GSE may should activity against certain microorganisms, but I have

>never seen ANY data that would suggest that GSE is an effective

>preservative for cosmetics. The efficacy of preservatives is

>determined through

>

>Some GSE products have been adulterated with antimicrobials. This may

>be so. But just because GSE may be effective killing a handful of

>microorganisms does not make it effective as a cosmetic preservative.

>

>An effective preservative is one that is capable of passing a

>preservative efficacy testing, sometimes called a preservative

>challenge test.

>

>The FDA's Bacteriological Analytical Manual (BAM)

><http://www.cfsan.fda.gov/~ebam/bam-toc.html> presents the agency's

>preferred laboratory procedures for microbiological analyses of foods

>and cosmetics.

>

>Quoting from the section entitled Microbiological Methods for Cosmetics

><http://www.cfsan.fda.gov/~ebam/bam-23.html>:

>

> " Cosmetic products are not expected to be aseptic; however, they must

>be completely free of high-virulence microbial pathogens, and the total

>number of aerobic microorganisms per gram must be low. Since there are

>no widely acceptable standards for numbers, temporary guidelines are

>used instead. For eye-area products, counts should not be greater than

>500 colony forming units (CFU)/g; for non-eye-area products, counts

>should not be greater than 1000 CFU/g. The presence of pathogens would

>be particularly important in evaluating as unacceptable a cosmetic with

>a marginally acceptable count, e.g., 400 CFU/g for an eye-area product.

>Pathogens or opportunistic pathogens whose incidence would be of

>particular concern, especially in eye-area cosmetic products, include

>S. aureus, Streptococcus pyogenes, P. aeruginosa and other species, and

>Klebsiella pneumoniae. Some microbes normally regarded as nonpathogenic

>may be opportunistically pathogenic, e.g., in wounds.

>

>Cosmetic preservative efficacy.

>

>The above guidelines for interpretation of results apply to cosmetic

>products before the time of use. Cosmetics contain antimicrobial

>preservatives and thus are expected to withstand a certain amount of

>abuse by users. Formerly, there were no validated tests for cosmetic

>preservative efficacy, although the test for pharmaceutical

>preservative efficacy in the U.S. Pharmacopeia or the cosmetic test in

>the technical guidelines of the Cosmetic, Toiletry, and Fragrance

>Association (CTFA) were used. Recently, the CTFA test has been AOAC

>validated for use with liquid cosmetics. "

>

>In a preservative challenge test, multiple microorganisms, anywhere

>from 5 to 10, are added to the product/preservative system being

>tested. Hence, the product is being challenged. Then the product is

>tested over several time intervals to see how quickly the preservative

>system kills the microorganisms. As I recall, CTFA preservative

>efficacy test method uses 10 different microorganisms as part of the

>challenge and the product is tested after one hour, 7 days, 14 days and

>28 days. This requires special equipment and knowledge of

>microbiological techniques.

>You would think that for just a few hundred dollars, the manufacturers

>of GSE would be rushing to have this test conducted and the results

>published.

>

>And now, lets address the information that is found on Pat McKay's

>website

>http://www.patmckay.com/Products.html

>

>Actually, the information on GSE is found on

>http://www.patmckay.com/Product_11.html

>

>First of all, nowhere in this article does it say that GSE is an

>effective preservative for personal care products.

>

>Pat McKay and Nutriteam put information on their websites is incorrect

>and misleading.

>

>Why would anyone do that? I can come up with two possible reasons:

>

>1)They don't know that the information is incorrect and misleading

>

>2)The incorrect and misleading information is published to deceive.

>

>In either case, I wouldn't purchase a product from a company that is

>unfamiliar with the products they sell and I certainly wouldn't buy

>products from a company that tries to deceive me.

--------------------

>Below are abstracts of two interesting articles that appeared in the

>June 2002 issue of the Journal of Alternative and Complementary

>Medicine (New York, N.Y.)

>

>A new list member, " MarthaE " , brought these

>two artcles to my attention and asked for my comments:

>

>-------------------

>The effectiveness of processed grapefruit-seed extract as an

>antibacterial agent: I. An in vitro agar assay.

>

>Reagor L, Gusman J, McCoy L, Carino E, Heggers JP.

>

>J Altern Complement Med. 2002 Jun;8(3):325-32.

>

>School of Medicine, University of Texas, Medical Branch, Galveston,

>USA.

>

>OBJECTIVES: Grapefruit-seed extract (GSE) Citricidal has, in recent

>reports, been reported to be successful in combating a variety of

>common infectious agents. In our study, drops of concentrated

>grapefruit-seed extract were tested for antibacterial properties

>against a number of gram-positive and gram-negative organisms.

>

>DESIGN: Sixty-seven (67) distinct biotypes were tested for their

>susceptibilities to the GSE as well as to 5 other topical

>antibacterials (Silvadene, Sulfamylon, Bactroban, Nitrofurazone, and

>Silvadene, Nystatin). Wells were punched into Mueller-Hinton agar

>plates, which were then inoculated with the organism to be tested; each

>well was then inoculated with one of the antibacterial agents. After an

>overnight incubation period, the plates were checked for zones of

>bacterial susceptibility around the individual wells, with a measured

>susceptibility zone diameter of 10 mm or more considered a positive

>result.

>

>RESULTS: The GSE was consistently antibacterial against all of the

>biotypes tested, with susceptibility zone diameters equal to or greater

>than 15 mm in each case.

>

>CONCLUSIONS: Our preliminary data thus suggest an antibacterial

>characteristic to GSE that is comparable to that of proven topical

>antibacterials. Although the GSE appeared to have a somewhat greater

>inhibitory effect on gram-positive organisms than on gram-negative

>organisms, its comparative effectiveness against a wide range of

>bacterial biotypes is significant.

>

>-------------------

>The effectiveness of processed grapefruit-seed extract as an

>antibacterial agent: II. Mechanism of action and in vitro toxicity.

>

>Heggers JP, Cottingham J, Gusman J, Reagor L, McCoy L, Carino E, R,

>Zhao JG.

>

>J Altern Complement Med. 2002 Jun;8(3):333-40.

>

>Department of Surgery (Plastic), School of Medicine, University of

>Texas Medical Branch, Galveston, USA. jphegger@...

>

>OBJECTIVES: Recent testimonials report grapefruit-seed extract, or GSE

>(Citricidal) to be effective against more than 800 bacterial and viral

>strains, 100 strains of fungus, and a large number of single and

>multicelled parasites. This study investigated GSE for antibacterial

>activity at varying time intervals and concentration levels and tissue

>toxicity at varying concentrations in an effort to determine if a

>concentration existed that was both microbicidal and nontoxic and in

>what period of time.

>

>DESIGN: Gram-negative and gram-positive isolates were introduced into

>graduated dilutions of GSE (twofold concentrations ranging from 1:1,

>through 1:512) for determination of bacterial activity. In vitro assays

>with human skin fibroblast cells were also performed at the same

>dilutions to determine toxicity.

>

>RESULTS: These tests indicated that from the 1:1 through the 1:128

>concentrations, GSE remained toxic as well as bactericidal. However,

>test results indicated that at the 1:512 dilution, GSE remained

>bactericidal, but completely nontoxic.

>

>CONCLUSIONS: The initial data shows GSE to have antimicrobial

>properties against a wide range of gram-negative and gram-positive

>organisms at dilutions found to be safe. With the aid of scanning

>transmission electron microscopy (STEM), the mechanism of GSE's

>antibacterial activity was revealed. It was evident that GSE disrupts

>the bacterial membrane and liberates the cytoplasmic contents within 15

>minutes after contact even at more dilute concentrations.

>-------------------

>

>Based on the limited data available on the Citricidal websites, I've

>always felt that Citricidal had some " antimicrobial activity " and these

>two articles that were published in the Journal of Alternative and

>Complementary Medicine only provide more support.

>

>However, just because a raw material or ingredient has antimicrobial

>activity does not mean that same item is an effective preservative for

>cosmetics. Methylparaben has antimicrobial activity but Methylparaben,

>by itself, is not an effective preservative for cosmetics.

>

>And so too, just because Citricidal has demonstrated antimicrobial

>activity against some undefined microorganisms does not mean that

>Citricidal, by itself, is an effective preservative.

>

>Methylparaben is used as just one component in many multi-component

>preservative systems. Perhaps, Citricidal could be used as just one

>component of a multi-component preservative system.

>

>In your quest for a natural preservatives system, one of the most

>difficult jobs will be defining what is mean by the word " natural " .

>

>I don't believe Citricidal, by itself, is an effective cosmetic

>preservative nor does it fit my personal definition of the term

> " natural " .

--------------------

>While I was searching the CTFA dictionary, I also found a listing for " Citrus

Grandis (Grapefruit) Fruit Extract " which is an extract of the fruit of the

grapefruit, Citrus grandis.

>

>According to the dictionary, the INCI name for Citricidal is " Citrus Grandis

(Grapefruit) Fruit Extract " . I always thought that Citricidal was GSE.

--------------------

>

>

>>I don't usually enter into discussions of GSE because I'm sick of them (LOL)

but

>>have to say that Citricidal have been using organic grapefruit for a long time

>>now!

>>

>>I have creams and lotions that are 9 months old now and preserved with only

GSE

>>and ROE. They have been lab tested by my local lab and are as good and clean

as

>>the day they were made.

>

>The only way to tell if a preservative or preservative system is

>effective is with a preservative efficacy test or challenge test.

>

>Sounds like you just has an aerobic plate count (APC) performed on your

>product. It's relatively easy to get low scores from an APC.

>Unfortunately, a low score on an APC is not an indicatition that the

>same product will pass a challenge test.

>

>I've searched and I have never seen any challange test data that would

>make me believe that GSE is an effective preservative. If Citricidal

>was an effective preservative, you would think that the manufacturer

>would publish the challenge test result. As I recall, the Citricidal

>website says that Citricidal is used as a preservative but never say

>that Citricidal has ever passed a challenge test.

>

>I haven't checked Aveda product line lately, but they had GSE listed in

>their ingredient list along with Propylene Glycol, olidinyl Urea,

>Methylparaben and Propylparaben.

>

>If you are selling any cosmetic product containing GSE as the only

>preservative, you should re-think your decision. You are exposing your

>company to liability.

--------------------

Maurice

--------------------------------------------------------

Maurice O. Hevey

Convergent Cosmetics, Inc.

http://www.ConvergentCosmetics.com

-------------------------------------------------------

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