Re: Kawashima's abstract

[Guest guest]

--

Would that not be so if my children have titers for both measles

from the MMR? But I know they do not have titers for the mump part.

Kerri

- In @y..., D Bogert <howard78@j...> wrote:

> Cece,

> I think this is what you're looking for...

>

> > DIGESTIVE DISEASES AND SCIENCES 2000 Apr;45(4):723-9

> > Detection and sequencing of measles virus from peripheral

mononuclear

> > cells from patients with inflammatory bowel disease and autism.

> >

> > Kawashima H, Mori T, Kashiwagi Y, Takekuma K, Hoshika A,

Wakefield A

> > Department of Paediatrics, Tokyo Medical University, Japan.

> >

> > It has been reported that measles virus may be present in the

intestine

> > of patients with Crohn's disease. Additionally, a new syndrome

has been

> > reported in children with autism who exhibited developmental

regression

> > and gastrointestinal symptoms (autistic enterocolitis), in some

cases

> > soon after MMR vaccine.

> >

> > It is not known whether the virus, if confirmed to be present in

these

> > patients, derives from either wild strains or vaccine strains. In

order

> > to characterize the strains that may be present, we have carried

out

> the

> > detection of measles genomic RNA in peripheral mononuclear cells

(PBMC)

> > in eight patients with Crohn's disease, three patients with

ulcerative

> > colitis, and nine children with autistic enterocolitis. As

controls, we

> > examined healthy children and patients with SSPE, SLE, HIV-1 (a

total

> of

> > eight cases).

> >

> > RNA was purified from PBMC by Ficoll-paque, followed by reverse

> > transcription using AMV; cDNAs were subjected to nested PCR for

> > detection of specific regions of the hemagglutinin (H) and fusion

(F)

> > gene regions. Positive samples were sequenced directly, in

nucleotides

> > 8393-8676 (H region) or 5325-5465 (from noncoding F to coding F

> region).

> > One of eight patients with Crohn disease, one of three patients

with

> > ulcerative colitis, and three of nine children with autism, were

> > positive. Controls were all negative.

> >

> > The sequences obtained from the patients with Crohn's disease

shared

> the

> > characteristics with wild-strain virus. The sequences obtained

from the

> > patients with ulcerative colitis and children with autism were

> > consistent with being vaccine strains. The results were

concordant with

> > the exposure history of the patients. Persistence of measles

virus was

> > confirmed in PBMC in some patients with chronic intestinal

> inflammation.