Protozoa / Sequencing the genome from the human pathogen Babesia microti

[Guest guest]

----- Forwarded Message ----To: LymeInfo Sent: Fri, July 27, 2012 7:17:42 AMSubject: [LymeInfo] [sci] Sequencing the genome from the human pathogen Babesia microti

Sequencing of the smallest Apicomplexan genome from the human pathogen Babesia microti{dagger}Cornillot E, Hadj-Kaddour K, Dassouli A, Noel B, Ranwez V, Vacherie B, Augagneur Y, Bres V, Duclos A, Randazzo S, Carcy B, Debierre-Grockiego F, Delbecq S, Moubri-Menage K, Shams-Eldin H, Usmani-Brown S, Bringaud F, Wincker P, Vivares CP, Schwarz RT, Schetters TP, Krause PJ, Gorenflot A, Berry V, Barbe V, Ben Mamoun C.Nucleic Acids Research, online before print, 2012 Jul 24.http://dx.doi.org/10.1093/nar/gks700AbstractWe have sequenced the genome of the emerging human pathogen Babesia microti and compared it with that of other protozoa. B. microti has the smallest nuclear genome among all Apicomplexan parasites sequenced to date with three chromosomes encoding ∼3500 polypeptides, several of which are species specific.Genome-wide phylogenetic analyses indicate that B. microti

is significantly distant from all species of Babesidae and Theileridae and defines a new clade in the phylum Apicomplexa. Furthermore, unlike all other Apicomplexa, its mitochondrial genome is circular. Genome-scale reconstruction of functional networks revealed that B. microti has the minimal metabolic requirement for intraerythrocytic protozoan parasitism. B. microti multigene families differ from those of other protozoa in both the copy number and organization. Two lateral transfer events with significant metabolic implications occurred during the evolution of this parasite.The genomic sequencing of B. microti identified several targets suitable for the development of diagnostic assays and novel therapies for human babesiosis.http://dx.doi.org/10.1093/nar/gks700This is an Open Access article

distributed under the terms of the Creative Commons Attribution Non-Commercial Licensehttp://creativecommons.org/licenses/by-nc/3.0which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Full Text pdf file:http://nar.oxfordjournals.org/content/early/2012/07/23/nar.gks700.full.pdf+html