oxidative stress

[Guest guest]

Ogunro PS, Ogungbamigbe TO, Ajala MO, Egbewale BE. Total antioxidant status

and lipid peroxidation in HIV-1 infected patients in a rural area of south

western Nigeria. Afr J Med Med Sci. 2005 Sep;34(3):221-5.

Department of Chemical Pathology, College of Health Sciences, Ladoke

Akintola University of Technology, Osogbo, Nigeria. ogunrops@...

Total serum antioxidant status (TAS) and lipid peroxidation were evaluated

in 62 HIV-1 seropositive patients before the commencement of antiretroviral

drug therapy. Twenty-four (24) age-marched and apparently healthy HIV-1

seronegative subjects were recruited as control subjects. HIV-1

seropositive patients were classified according to the Centre for Disease

Control and Prevention (CDC) Criteria. The mean serum malondialdehyde (MDA)

concentrations of 5.58 +/- 0.99nmol/ml and 4.24 +/- 0.80nmol/ml were

significantly elevated in HIV-1 infected patients with CD4+ count of <200

lym/mm3 and that of 200-499 lym/mm3 respectively when compared with the

control which is 3.37 +/- 0.56nmol/ ml (P<0.001). Whereas when compared

with control, the serum MDA concentration of 3.45 +/- 0.48nmol/ml in HIV-1

patients with CD4+ count of >500 lym/mm3 was not significant (P>0.05). In

the intra groups comparison serum concentration of MDA were significantly

elevated when compared with each other (P<0.001). The mean serum total

antioxidant status (TAS) of 1.30 +/- 0.11mmol/L, 1.12 +/- 0.24 mmol/L and

0.95 +/- 0.17mmol/L of HIV- 1 seropositive patients with CD4+ count of >500

lym/mm3, 200-499 lym/mm3 and <200 lym/mm3 respectively were significantly

reduced compared with 1.69 +/- 0.23mmol/L for the control group (P<0.001).

Similarly in the intra groups comparison, serum concentration of TAS were

significantly reduced when compared with each other (P<0.001). The weight

of 54.81 +/- 5.13 Kg for HIV-I seropositive patients with CD4+ count of

<200 lym/mm3 was significantly reduced when compared with 69.17 +/- 4.38Kg

for the control (P<0.05). Our results clearly show that severe oxidative

stress occurs in the serum of seropositive patients in comparison with

controls, and increases significantly with the progression of disease, i.e.

AIDS > symptomatics > asymptomatics > controls. The inclusion of

antioxidants in the therapeutic approach in managing HIV- 1 seropositive

patients will prevent the additional damage that free radicals could do to

such patients.