RE: Re: ..probiotics no good bacteria

[Guest guest]

Ok so this bacterium BT eats the gut

flora then opens the spores releases the toxin then kills your digestive

enzymes and then causes leaky gut, the bacterium itself won’t kill you

nor the bugs but the co-infections our children acquire because of this messy

bacterium that has been genetically modified yes! It has similar consequences

than aids in the sense that impairs the ability for our children to fight

infections, and it’s the same way it happens to poor bees that are disappearing

as you had heard! That’s right the bees are disappearing because they are

the first exposed to this poisonous food then our children and us go next! Right

now it’s my hypothesis because I can’t find a lab to cooperate, but

it clairly explains the missing gut flora in our children and lack of enzymes

in a logical reason to me.

.

Midgut bacteria required for Bacillus thuringiensis

insecticidal activity

Nichole A. Broderick*†‡, F. Raffa*, and Jo Handelsman†§

Departments

of *Entomology and †Plant

Pathology and ‡Microbiology

Doctoral Training Program, University

of Wisconsin, 1630 Linden Drive,

Madison,

WI 53706

Edited

by I. Gordon, Washington University School of Medicine, St. Louis, MO,

and approved August 17, 2006 (received for review June 12, 2006)

plants

engineered

with

the cry genes encoding the B. thuringiensis crystal proteins

are

the most widely cultivated transgenic crops. For decades, the

mechanism of

insect killing has been assumed to be toxin-mediated

lysis of the gut

epithelial cells, which leads to starvation, or B.

thuringiensis

septicemia. Here, we report

that B. thuringiensis does

not

kill larvae of the gypsy moth in the absence of indigenous

midgut

bacteria.

Elimination

of the gut microbial community by

oral

administration of antibiotics abolished B.

thuringiensis insecticidal

activity,

and reestablishment of an Enterobacter sp.

that

normally

resides in the midgut microbial community restored B.

thuringiensis-mediated

killing.

Escherichia coli engineered

to produce the B. thuringiensis insecticidal

toxin killed gypsy moth larvae

irrespective

of the presence of other bacteria in the midgut.

Laboratory Tests of Acute Toxicity

Each of the more than 800 strains of Bacillus thuringiensis may exhibit

different toxicity to insects, rodents and humans. This fact

complicates any discussion about the toxicity of Bt The following are summaries of the acute toxicity data

available for two commonly used commercial varieties of Bt. In a purified form, Bti.'s endotoxin is clearly toxic to

mammals. Bt is widely used in

cotton production in Texas, Mississippi,

and Louisiana as well as in the production of

fruits and vegetables in California, Arizona, and Florida.

In California, where pesticide use reporting is

more comprehensive than in other states, almost 52,000 pounds of Bt

were used on diverse crops in 1991. Grapes, lettuce, and tomatoes account for almost half the Bt used in California (fig. 2).

Bt is extensively used nationawide in the production of certain fruit

and vegetable crops (fig. 3).

Monitoring studies following

large-scale Bt spray programs

have shown that ex-posed people carry Bt

in their tissues. For example, more than 11 percent of nasal swab samples taken

from patients surveyed by doctors in Vancouver (Canada)

following a gypsy moth spray program were found to contain Btk.23 Bt

was also found in cultures taken from patients in Lane County, Oregon following

a gypsy moth spray program there. Monitoring studies also show that exposed

people report a variety of health problems that they believe to be associated

with Bt exposure.22 For example, during the Vancouver spray pro-gram,

almost 250 people reported health problems, mostly allergy-like or flu-like

symptoms. During a Washington

gypsy moth spray program, over 250 people re-ported health problems and 6 were

treated in emergency rooms for allergy or asthma problems.26 Physicians have so far been un-able

to definitively link Bt exposure

to these health problems.22,23,26

Mode of Action

When conditions for bacterial growth

are not optimal Bt, like many

bacteria, forms spores. Spores are the dormant stage of the bacterial life

cycle, when the organism

waits for better growing conditions. Unlike many other bacteria,

when Bt creates spores it also

creates a protein crystal. This crystal is the toxic component of Bt.

After the insect ingests Bt, the crystal is dissolved in the

insect's alkaline gut. Then

the insect's digestive enzymes break down the crystal structure and activate Bt's insecticidal component,

called the delta-endotoxin. The delta-endotoxin binds to the cells lining the midgut

membrane and creates pores in the membrane, upsetting the gut's ion balance.

The insect soon stops feeding and starves to death. If the insect is not

susceptible to the direct action of the delta-endotoxin, death occurs after Bt starts vegetative growth inside the

insect's gut. The spore germinates after the gut membrane is broken; it then

reproduces and makes more spores. This body-wide infection eventually kills the

insect.8

Special Concerns about Bt Toxicity

Exotoxins:

The earliest tests done regarding Bt's

toxicity were conducted using Bt

var. thuringiensis, a Bt strain

known to contain a second toxin called beta-exotoxin. The beta-exotoxin is

toxic to vertebrates, with an LD 50 (median lethal dose; the dose that kills 50

percent of a population of test animals) of 13-18 milligrams per kilogram of

body weight (mg/kg) in mice when injected into the abdomen. An oral dose of 200

mg/kg per day killed mice after eight days.20 Beta-exotoxin also causes genetic damage to human

blood cells.27 Bt formulations containing beta-exotoxin

have not been used in most countries 20

although attempts are currently being made to register beta-exotoxin as an

insecticide in the United States.8 Another toxin produced by Bt is the alpha-exotoxin that is highly

acutely toxic to mice.20

Current Bt production methods are

such that alpha-exotoxin is not a " significant component " of Bt formulations.8

Related Bacteria:

Bt belongs to a small group of

closely related Bacillus species, including B. cereus, a bacteria that is an

agent of food poisoning, and B. anthracis, the pathogen of the virulent animal

disease, anthrax. These three bacteria are so similar it has been theorized

that they are all varieties of the same species.28,29 If B. cereus is cultured with Bt cells, genetic material is transferred

to the B. cereus cells that allows B. cereus to produce Bt's crystal proteins.28 Transfers of genetic material

between B. anthracis and Bt have

also occurred.30

A toxin produced by B. cereus that

causes diarrhea in monkeys is also produced by certain strains of Bt,30

although this toxin is not likely to be present in Bt spore formulations.28

Human volunteers suffered from nausea, vomiting, diarrhea, colic-like pains,

and fever after eating food contaminated with one Bt strain, Bt

var. galleriae.31 These examples indicate the close

relationship between Bt and

disease-causing pathogens.

Increased Susceptibility: People with

compromised immune systems or preexisting allergies may be particularly

susceptible to the effects of Bt

In mice with reduced immune function, the dose required to kill more than 50

percent of the mice when injected was several orders of magnitude smaller than

the highest dose tested in nor-mal mice.32

Mice with impaired immune function also showed higher mortality than regular

mice when one dose of Bti. was

injected into the abdominal cavity.33

Although no definite cases have been reported of Bt infecting humans with compromised immune systems, the

Oregon Health Division suggested before a Btk.

spray pro-gram that " individuals with...physician-diagnosed causes of

severe immune disorders may consider leaving the area during the actual spraying. " 34

A memo from Novo Nordisk, the

manufacturer of Foray 48B, states that the amount of the spray a person would

be exposed to would be too small to develop new allergies. However, " It is

possible that someone that already has developed an allergy to one of the

components of Foray 48B or has asthma ... could be affected by exposure to

small quantities of Foray 48B. " 35

The 1991 Material Safety Data Sheet for Foray 48B states " Repeated

expo-sure via inhalation can result in sensitization and allergic response in

hypersensitive individuals. " 36

Contaminants: In the mid 1980s,

several Bt products were

contaminated with other bacteria, including Streptococcus faecium and S.

faecalis.37 While Bt products are

routinely monitored for bacterial contaminants,2 the risk of contamination with

a disease-causing bacteria is always present.25

From:

BorreliaMultipleInfectionsAndAutism

[mailto:BorreliaMultipleInfectionsAndAutism ] On Behalf Of aaron2kristie

Sent: Sunday, March 09, 2008 5:17

PM

To:

BorreliaMultipleInfectionsAndAutism

Subject:

Re: ..probiotics no good bacteria

I have 2 kids, and I can tell you the gastrointestinal

problems

ocurrs first, before the autistic characteristics showed up, and also

ask yourself

where the probiotics are going to after all of us keep pumping

millions per capsule every day and the kids still show " no good

flora " in the lab results, something has to be killing them! Or where

are these going to? Well I have strong evidence that proves BT toxin

feeds on friendly bacteria to keep producing the poisoning toxins;

also I have connection to the casein effect.

,

Please talk more about this. You could be speaking about my son. I

pumped him full of probiotics and zero lactobicillus would continue

to show on lab work. I started with a DAN physician doing this in

September of 2005 when he was but 2 years 3 months old. We did this

over two years and still zero lactobicillus even with the strongest

probiotic like VSL#3. Help me explain (being as non technical as you

can because I am not close to being scientist mommy at all!) in

laymans terms this process.

Kristie

Aidan age 4 non verbal, autism and apraxia

[Guest guest]

, I agree with your conclusions that the decline in bees and other

insects is due to altered gut flora …I,m not sure that the BT theory squares

the situation ..mainly because the decline is world [western] wide and I don’t think the bacterium is used

in agriculture world wide .

I have been in correspondence with a professor entomologist ..some of the exchange. below

..I have more info on this subject if you or anyone is interested …

Subject: RE: Decline and Conservation of Bumble Bees

Hello again Dave , I

hope you have had time to read my site , if you have you will see the main

theme is antibiotics have impacted in a very destructive way on our gut flora ,

altering the balance etc;etc;

Well , as most

antibiotics by far , some 80% are given to animals , [in reality far more are

consumed there is a flourishing black market ]I reason that they will be

similarly affected .. I have some data that supports that . but it's the knock

on effect what is probably the most potentially damaging ..We know that organic

farms host the most varied wild life , that includes insects ..I put the

argument that the decline in wild life could be antibiotics/ pesticides

impacting in the equally important gut flora of animals and insect ..

Take a look at the link I've provided , and get back to me with

your comments . Cheers ..

http://www.ens-newswire.com/ens/jul2007/2007-07-12-01.asp

antibiotics inpact on

bacteria allowing fungi to flourish ..

http://www.theregister.co.uk/2007/04/27/ccd_fungus_link/

Hi ,

Sorry for slow reply - I haven't really had time to look into this!

I

certainly buy the idea that prophylactic use of antibiotics in farm

animals is likely to have widespread effects on e.g. soil microbes,

but

I'm not aware of any known symbiotic bacteria that bees have, hence

I

remain unconvinced that there is a direct effect on bees (I'm not

saying

there isn't, I have seen no evidence either way). It remains an

interesting idea! The recent collapses in bee colonies in the US

are

thought to be due to viral epidemics.

Merry Xmas

Dave

-----Original

Message-----

From:

BorreliaMultipleInfectionsAndAutism

[mailto:BorreliaMultipleInfectionsAndAutism ]On Behalf Of

Sent: 11 March 2008 01:28

To:

BorreliaMultipleInfectionsAndAutism

Subject: RE:

Re: ..probiotics no good bacteria

Ok so this

bacterium BT eats the gut flora then opens the spores releases the toxin then

kills your digestive enzymes and then causes leaky gut, the bacterium itself

won’t kill you nor the bugs but the co-infections our children acquire because

of this messy bacterium that has been genetically modified yes! It has similar

consequences than aids in the sense that impairs the ability for our children

to fight infections, and it’s the same way it happens to poor bees that are

disappearing as you had heard! That’s right the bees are disappearing because

they are the first exposed to this poisonous food then our children and us go

next! Right now it’s my hypothesis because I can’t find a lab to cooperate, but

it clairly explains the missing gut flora in our children and lack of enzymes

in a logical reason to me.

.

Midgut bacteria required for Bacillus thuringiensis

insecticidal activity

Nichole

A. Broderick*†‡, F. Raffa*, and Jo

Handelsman†§

Departments of

*Entomology and †Plant Pathology and ‡Microbiology

Doctoral Training Program, University of Wisconsin,

1630 Linden Drive,

Madison, WI

53706

Edited by

I. Gordon, Washington University School of Medicine, St. Louis,

MO, and approved August 17, 2006 (received for review June 12,

2006)

plants engineered

with the cry genes

encoding the B. thuringiensis crystal

proteins

are the most widely cultivated transgenic crops. For decades, the

mechanism

of insect killing has been assumed to be toxin-mediated

lysis

of the gut epithelial cells, which leads to starvation, or B.

thuringiensis septicemia.

Here, we report that B. thuringiensis does

not kill larvae of the gypsy moth in the absence of

indigenous

midgut bacteria.

Elimination of the gut microbial community by

oral administration of antibiotics abolished B. thuringiensis insecticidal

activity, and reestablishment of an Enterobacter sp. that

normally resides in the midgut microbial community restored B.

thuringiensis-mediated killing.

Escherichia coli engineered to produce the B. thuringiensis insecticidal toxin killed gypsy moth larvae

irrespective of the presence of other bacteria in the midgut.

Laboratory

Tests of Acute Toxicity

Each of

the more than 800 strains of Bacillus

thuringiensis may exhibit different toxicity to insects, rodents and humans. This fact

complicates any discussion about the toxicity of Bt The following are summaries of the acute toxicity data

available for two commonly used commercial varieties of Bt. In a purified form, Bti.'s endotoxin is clearly toxic to

mammals. Bt is widely used in

cotton production in Texas, Mississippi, and Louisiana

as well as in the production of fruits and vegetables in California,

Arizona, and Florida.

In California, where

pesticide use reporting is more comprehensive than in other states, almost 52,000 pounds of Bt were used on diverse crops in 1991.

Grapes, lettuce, and

tomatoes account for almost half the Bt

used in California (fig. 2).

Bt is extensively used nationawide in the production of certain fruit

and vegetable crops (fig. 3).

Monitoring studies following large-scale Bt spray programs have shown that ex-posed

people carry Bt in their tissues.

For example, more than 11 percent of nasal swab samples taken from patients

surveyed by doctors in Vancouver (Canada)

following a gypsy moth spray program were found to contain Btk.23 Bt

was also found in cultures taken from patients in Lane County, Oregon following

a gypsy moth spray program there. Monitoring studies also show that exposed

people report a variety of health problems that they believe to be associated

with Bt exposure.22 For example, during the Vancouver

spray pro-gram, almost 250 people reported health problems, mostly allergy-like

or flu-like symptoms. During a Washington gypsy moth

spray program, over 250 people re-ported health problems and 6 were treated in

emergency rooms for allergy or asthma problems.26 Physicians have so far been un-able to definitively

link Bt exposure to these health

problems.22,23,26

Mode of Action

When conditions for bacterial growth are

not optimal Bt, like many

bacteria, forms spores. Spores are the dormant stage of the bacterial life

cycle, when the organism

waits for better growing conditions. Unlike many other bacteria,

when Bt creates spores it also

creates a protein crystal. This crystal is the toxic component of Bt.

After the insect ingests Bt, the crystal is dissolved in the

insect's alkaline gut. Then

the insect's digestive enzymes break down the crystal structure and activate Bt's insecticidal component,

called the delta-endotoxin. The delta-endotoxin binds to the cells lining the

midgut membrane and creates pores in the membrane, upsetting the gut's ion

balance. The insect soon stops feeding and starves to death. If the insect is

not susceptible to the direct action of the delta-endotoxin, death occurs after

Bt starts vegetative growth

inside the insect's gut. The spore germinates after the gut membrane is broken;

it then reproduces and makes more spores. This body-wide infection eventually

kills the insect.8

Special

Concerns about Bt Toxicity

Exotoxins:

The earliest tests done regarding Bt's

toxicity were conducted using Bt

var. thuringiensis, a Bt strain known

to contain a second toxin called beta-exotoxin. The beta-exotoxin is toxic to

vertebrates, with an LD 50 (median lethal dose; the dose that kills 50 percent

of a population of test animals) of 13-18 milligrams per kilogram of body

weight (mg/kg) in mice when injected into the abdomen. An oral dose of 200

mg/kg per day killed mice after eight days.20 Beta-exotoxin also causes genetic damage to human

blood cells.27 Bt formulations containing beta-exotoxin

have not been used in most countries 20

although attempts are currently being made to register beta-exotoxin as an

insecticide in the United States.8 Another toxin produced by Bt is the alpha-exotoxin that is highly

acutely toxic to mice.20

Current Bt production methods are

such that alpha-exotoxin is not a " significant component " of Bt formulations.8

Related Bacteria:

Bt belongs to a small group of

closely related Bacillus species, including B. cereus, a bacteria that is an

agent of food poisoning, and B. anthracis, the pathogen of the virulent animal disease,

anthrax. These three bacteria are so similar it has been theorized that they

are all varieties of the same species.28,29

If B. cereus is cultured with Bt

cells, genetic material is transferred to the B. cereus cells that allows B.

cereus to produce Bt's crystal

proteins.28 Transfers

of genetic material between B. anthracis and Bt

have also occurred.30

A toxin produced by B. cereus that causes

diarrhea in monkeys is also produced by certain strains of Bt,30

although this toxin is not likely to be present in Bt spore formulations.28

Human volunteers suffered from nausea, vomiting, diarrhea, colic-like pains,

and fever after eating food contaminated with one Bt strain, Bt

var. galleriae.31 These examples indicate the close

relationship between Bt and disease-causing

pathogens.

Increased Susceptibility: People with

compromised immune systems or preexisting allergies may be particularly

susceptible to the effects of Bt

In mice with reduced immune function, the dose required to kill more than 50

percent of the mice when injected was several orders of magnitude smaller than

the highest dose tested in nor-mal mice.32

Mice with impaired immune function also showed higher mortality than regular

mice when one dose of Bti. was

injected into the abdominal cavity.33

Although no definite cases have been reported of Bt infecting humans with compromised immune systems, the

Oregon Health Division suggested before a Btk.

spray pro-gram that " individuals with...physician-diagnosed causes of

severe immune disorders may consider leaving the area during the actual

spraying. " 34

A memo from Novo Nordisk, the manufacturer

of Foray 48B, states that the amount of the spray a person would be exposed to

would be too small to develop new allergies. However, " It is possible that

someone that already has developed an allergy to one of the components of Foray

48B or has asthma ... could be affected by exposure to small quantities of

Foray 48B. " 35 The

1991 Material Safety Data Sheet for Foray 48B states " Repeated expo-sure

via inhalation can result in sensitization and allergic response in

hypersensitive individuals. " 36

Contaminants: In the mid 1980s, several Bt products were contaminated with other

bacteria, including Streptococcus faecium and S. faecalis.37 While Bt products are routinely monitored for

bacterial contaminants,2 the risk of contamination with a disease-causing

bacteria is always present.25

From: BorreliaMultipleInfectionsAndAutism

[mailto:BorreliaMultipleInfectionsAndAutism ]

On Behalf Of aaron2kristie

Sent: Sunday, March 09, 2008 5:17

PM

To: BorreliaMultipleInfectionsAndAutism

Subject:

Re: ..probiotics no good bacteria

I have 2 kids, and I can tell you the gastrointestinal problems

ocurrs first, before the autistic characteristics showed up, and also

ask yourself

where the probiotics are going to after all of us keep pumping

millions per capsule every day and the kids still show " no good

flora " in the lab results, something has to be killing them! Or where

are these going to? Well I have strong evidence that proves BT toxin

feeds on friendly bacteria to keep producing the poisoning toxins;

also I have connection to the casein effect.

,

Please talk more about this. You could be speaking about my son. I

pumped him full of probiotics and zero lactobicillus would continue

to show on lab work. I started with a DAN physician doing this in

September of 2005 when he was but 2 years 3 months old. We did this

over two years and still zero lactobicillus even with the strongest

probiotic like VSL#3. Help me explain (being as non technical as you

can because I am not close to being scientist mommy at all!) in

laymans terms this process.

Kristie

Aidan age 4 non verbal, autism and apraxia