Re: Interesting abstract on borrelia, need comments please

[Guest guest]

Natasa:

Check out the abstract on this link. It says that microvascular

permeability increases 4-fold when female hormones are lowered. I

wonder if this is a big contributor why females get sick at age 40

and men get sick soon after birth. My mother died in 1990 from what

I suspect was lyme and coinfections. The doctors never gave her any

diagnosis in particular, but did say the had leaky vessels and that

her blood was super thick. They said her vessels/veins were leaking

fluid into her body. It was so bad that they could not get her

pulse, yet she would talk to them. She got sick at 40 and died at

50. They just told us it was natural causes. I suspect lyme runs

throughout my family. Both her father and my father were avid deer

hunters. Mom even went on hunting trips herself.

http://ajpheart.physiology.org/cgi/content/full/293/2/H1131

I imagine that this condition of leaking vessels definitely would

hamper the body from functioning correctly, and leading to pathogen

and toxin overload. Thus, taking supplements that can reverse this,

would likely be of high benefit. As a matter of fact, I have come

across reports from time to time that certain supplements do " repair "

the lining to blood vessels. I am not knowledgeable about virulent

strains, but if I find out which strain I have, I will let you know.

But, I may have more than one strain for I have been around ticks

quite a lot.

Love and prayers,

Heidi N

>

>

> Hi Tami and others, I came across this recent paper abstract on

> borrelia, and found it quite interesting. it shows DIRECT damage

induced

> by bb (that is not through inflammatory cytokines produced by the

host

> defence system) on the vessels that line the blood. This offers an

> explanation of how bb could cause leaky gut and esp breakdown of

blood

> brain barrier.

>

> They notice that this damage is induced only by virulent strain of

bb,

> not by avirulent one. Are the lab lyme tests that are available at

the

> moment capable of establishing virulence of the strains found?

sorry if

> this is common knowledge, it is new to me

>

> also, when treating for lyme, is it possible/documented that bb

could be

> provoked to change into more virulent forms? there was a thread

> recently on 'awakening' lyme symptoms through supplements. Any

thoughts?

>

> Natasa

>

> PLoS ONE. 2008;3(12):e4101. Epub 2008 Dec 31. Spent culture

medium

> from virulent Borrelia burgdorferi increases permeability of

> individually perfused microvessels of rat mesentery. Zhou X

> <http://www.ncbi.nlm.nih.gov/sites/entrez?

Db=pubmed & Cmd=Search & Term=%22Z\

> hou%20X%22%5BAuthor%

5D & itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Results\

> Panel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlus> , ...Department

of

> Physiology and Pharmacology, West Virginia University, town,

WV,

> USA.

> BACKGROUND: Lyme disease is a common vector-borne disease caused by

the

> spirochete Borrelia burgdorferi (Bb), which manifests as systemic

and

> targeted tissue inflammation. Both in vitro and in vivo studies have

> shown that Bb-induced inflammation is primarily host-mediated, via

> cytokine or chemokine production that promotes leukocyte

> adhesion/migration. Whether Bb produces mediators that can directly

> alter the vascular permeability in vivo has not been investigated.

The

> objective of the present study was to investigate if Bb produces a

> mediator(s) that can directly activate endothelial cells resulting

in

> increases in permeability in intact microvessels in the absence of

blood

> cells. METHODOLOGY/PRINCIPAL FINDINGS: The effects of cell-free,

spent

> culture medium from virulent (B31-A3) and avirulent (B31-A) B.

> burgdorferi on microvessel permeability and endothelial calcium

> concentration, [Ca(2+)](i), were examined in individually perfused

rat

> mesenteric venules. Microvessel permeability was determined by

measuring

> hydraulic conductivity (Lp). Endothelial [Ca(2+)](i), a necessary

signal

> initiating hyperpermeability, was measured in Fura-2 loaded

> microvessels. B31-A3 spent medium caused a rapid and transient

increase

> in Lp and endothelial [Ca(2+)](i). Within 2-5 min, the mean peak Lp

> increased to 5.6+/-0.9 times the control, and endothelial [Ca(2+)]

(i)

> increased from 113+/-11 nM to a mean peak value of 324+/-35 nM. In

> contrast, neither endothelial [Ca(2+)](i) nor Lp was altered by B31-

A

> spent medium. CONCLUSIONS/SIGNIFICANCE: A mediator(s) produced by

> virulent Bb under culture conditions directly activates endothelial

> cells, resulting in increases in microvessel permeability. Most

> importantly, the production of this mediator is associated with Bb

> virulence and is likely produced by one or more of the 8 plasmid(s)

> missing from strain B31-A. PMID: 19116656

>

[Guest guest]

Natasa,

Thank you for your post. Hmmm... It seems that I need to increase the dosage of L-lysine again. L-lysine is involved in the structural repair of damaged blood vessels. Ingvar was on high dose vitamin C and L-lysine for a short period of time in 2006. That was my attempt to treat his CMV and EBV. He was doing really well at that time. Out of curiosity I collected his urine sample for UTM, when he was not chelated. His UTM reflected elevated level of Mercury without use of any metal chelator.

Limin

From: natasa778

Sent: Thursday, January 08, 2009 04:16

To: BorreliaMultipleInfectionsAndAutism

Subject: Interesting abstract on borrelia, need comments please

Hi Tami and others, I came across this recent paper abstract on borrelia, and found it quite interesting. it shows DIRECT damage induced by bb (that is not through inflammatory cytokines produced by the host defence system) on the vessels that line the blood. This offers an explanation of how bb could cause leaky gut and esp breakdown of blood brain barrier. They notice that this damage is induced only by virulent strain of bb, not by avirulent one. Are the lab lyme tests that are available at the moment capable of establishing virulence of the strains found? sorry if this is common knowledge, it is new to me :(also, when treating for lyme, is it possible/documented that bb could be provoked to change into more virulent forms? there was a thread recently on 'awakening' lyme symptoms through supplements. Any thoughts?Natasa

PLoS ONE. 2008;3(12):e4101. Epub 2008 Dec 31.

Spent culture medium from virulent Borrelia burgdorferi increases permeability of individually perfused microvessels of rat mesentery.

Zhou X, ...Department of Physiology and Pharmacology, West Virginia University, town, WV, USA.

BACKGROUND: Lyme disease is a common vector-borne disease caused by the spirochete Borrelia burgdorferi (Bb), which manifests as systemic and targeted tissue inflammation. Both in vitro and in vivo studies have shown that Bb-induced inflammation is primarily host-mediated, via cytokine or chemokine production that promotes leukocyte adhesion/migration. Whether Bb produces mediators that can directly alter the vascular permeability in vivo has not been investigated. The objective of the present study was to investigate if Bb produces a mediator(s) that can directly activate endothelial cells resulting in increases in permeability in intact microvessels in the absence of blood cells. METHODOLOGY/PRINCIPAL FINDINGS: The effects of cell-free, spent culture medium from virulent (B31-A3) and avirulent (B31-A) B. burgdorferi on microvessel permeability and endothelial calcium concentration, [Ca(2+)](i), were examined in individually perfused rat mesenteric venules. Microvessel permeability was determined by measuring hydraulic conductivity (Lp). Endothelial [Ca(2+)](i), a necessary signal initiating hyperpermeability, was measured in Fura-2 loaded microvessels. B31-A3 spent medium caused a rapid and transient increase in Lp and endothelial [Ca(2+)](i). Within 2-5 min, the mean peak Lp increased to 5.6+/-0.9 times the control, and endothelial [Ca(2+)](i) increased from 113+/-11 nM to a mean peak value of 324+/-35 nM. In contrast, neither endothelial [Ca(2+)](i) nor Lp was altered by B31-A spent medium. CONCLUSIONS/SIGNIFICANCE: A mediator(s) produced by virulent Bb under culture conditions directly activates endothelial cells, resulting in increases in microvessel permeability. Most importantly, the production of this mediator is associated with Bb virulence and is likely produced by one or more of the 8 plasmid(s) missing from strain B31-A. PMID: 19116656

[Guest guest]

Do you remember which ones, Heidi? Orthostatic Intolerance is

associated wiht " leaky veins. " Very interesting and possibly a

problem for me. Didnt get chronically sick until I hit 40. Robin

> >

> >

> > Hi Tami and others, I came across this recent paper abstract on

> > borrelia, and found it quite interesting. it shows DIRECT damage

> induced

> > by bb (that is not through inflammatory cytokines produced by the

> host

> > defence system) on the vessels that line the blood. This offers an

> > explanation of how bb could cause leaky gut and esp breakdown of

> blood

> > brain barrier.

> >

> > They notice that this damage is induced only by virulent strain

of

> bb,

> > not by avirulent one. Are the lab lyme tests that are available

at

> the

> > moment capable of establishing virulence of the strains found?

> sorry if

> > this is common knowledge, it is new to me

> >

> > also, when treating for lyme, is it possible/documented that bb

> could be

> > provoked to change into more virulent forms? there was a thread

> > recently on 'awakening' lyme symptoms through supplements. Any

> thoughts?

> >

> > Natasa

> >

> > PLoS ONE. 2008;3(12):e4101. Epub 2008 Dec 31. Spent culture

> medium

> > from virulent Borrelia burgdorferi increases permeability of

> > individually perfused microvessels of rat mesentery. Zhou X

> > <http://www.ncbi.nlm.nih.gov/sites/entrez?

> Db=pubmed & Cmd=Search & Term=%22Z\

> > hou%20X%22%5BAuthor%

> 5D & itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Results\

> >

Panel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlus> , ...Department

> of

> > Physiology and Pharmacology, West Virginia University,

town,

> WV,

> > USA.

> > BACKGROUND: Lyme disease is a common vector-borne disease caused

by

> the

> > spirochete Borrelia burgdorferi (Bb), which manifests as systemic

> and

> > targeted tissue inflammation. Both in vitro and in vivo studies

have

> > shown that Bb-induced inflammation is primarily host-mediated, via

> > cytokine or chemokine production that promotes leukocyte

> > adhesion/migration. Whether Bb produces mediators that can

directly

> > alter the vascular permeability in vivo has not been

investigated.

> The

> > objective of the present study was to investigate if Bb produces a

> > mediator(s) that can directly activate endothelial cells

resulting

> in

> > increases in permeability in intact microvessels in the absence

of

> blood

> > cells. METHODOLOGY/PRINCIPAL FINDINGS: The effects of cell-free,

> spent

> > culture medium from virulent (B31-A3) and avirulent (B31-A) B.

> > burgdorferi on microvessel permeability and endothelial calcium

> > concentration, [Ca(2+)](i), were examined in individually

perfused

> rat

> > mesenteric venules. Microvessel permeability was determined by

> measuring

> > hydraulic conductivity (Lp). Endothelial [Ca(2+)](i), a necessary

> signal

> > initiating hyperpermeability, was measured in Fura-2 loaded

> > microvessels. B31-A3 spent medium caused a rapid and transient

> increase

> > in Lp and endothelial [Ca(2+)](i). Within 2-5 min, the mean peak

Lp

> > increased to 5.6+/-0.9 times the control, and endothelial [Ca(2+)]

> (i)

> > increased from 113+/-11 nM to a mean peak value of 324+/-35 nM. In

> > contrast, neither endothelial [Ca(2+)](i) nor Lp was altered by

B31-

> A

> > spent medium. CONCLUSIONS/SIGNIFICANCE: A mediator(s) produced by

> > virulent Bb under culture conditions directly activates

endothelial

> > cells, resulting in increases in microvessel permeability. Most

> > importantly, the production of this mediator is associated with Bb

> > virulence and is likely produced by one or more of the 8 plasmid

(s)

> > missing from strain B31-A. PMID: 19116656

> >

>

[Guest guest]

I use to have Orthostatic Intolerance really really bad. Hmmm, I

wonder what made it improve. I got it at age 15, right after my

measles booster. I think it awakened my lyme or the vaccine, itself

made me ill or both. I was very very ill. The doctors just kept

wanting to give me pain pills and antidepressants. I took neither

and finally learned that if I don't eat sugar, nor caffeine, nor much

starches, and eat healthy, I could function. That lasted for about

10 years till I started getting what I thought was corpal tunnel

syndrome. Oh, well, enough of that long story. I think maybe the

diet in addition to gingko biloba made it go away. I know that

caffein really made it bad. I love chocolate, so I usually eat it

anyhow. I am actually getting a little bit of it returning since I

started the grapefruit seed extract. I think it's making my lyme

cysts go to lyme spirochetes, but who really knows. You know, I

haven't taken gingko in years, so I am thinking about taking it

again. I have some in the cubboard. Dr. B mentioned it in his new

guidelines for lyme treatment to dilate blood vessels.

I don't remember off-hand the herbs and supplements for leaky

veins/vessels, but I have been meaning to re-look that up.

Love and prayers,

Heidi N

> > >

> > >

> > > Hi Tami and others, I came across this recent paper abstract on

> > > borrelia, and found it quite interesting. it shows DIRECT

damage

> > induced

> > > by bb (that is not through inflammatory cytokines produced by

the

> > host

> > > defence system) on the vessels that line the blood. This offers

an

> > > explanation of how bb could cause leaky gut and esp breakdown

of

> > blood

> > > brain barrier.

> > >

> > > They notice that this damage is induced only by virulent strain

> of

> > bb,

> > > not by avirulent one. Are the lab lyme tests that are available

> at

> > the

> > > moment capable of establishing virulence of the strains found?

> > sorry if

> > > this is common knowledge, it is new to me

> > >

> > > also, when treating for lyme, is it possible/documented that bb

> > could be

> > > provoked to change into more virulent forms? there was a thread

> > > recently on 'awakening' lyme symptoms through supplements. Any

> > thoughts?

> > >

> > > Natasa

> > >

> > > PLoS ONE. 2008;3(12):e4101. Epub 2008 Dec 31. Spent

culture

> > medium

> > > from virulent Borrelia burgdorferi increases permeability of

> > > individually perfused microvessels of rat mesentery. Zhou X

> > > <http://www.ncbi.nlm.nih.gov/sites/entrez?

> > Db=pubmed & Cmd=Search & Term=%22Z\

> > > hou%20X%22%5BAuthor%

> > 5D & itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Results\

> > >

> Panel.Pubmed_DiscoveryPanel.Pubmed_RVAbstractPlus> , ...Department

> > of

> > > Physiology and Pharmacology, West Virginia University,

> town,

> > WV,

> > > USA.

> > > BACKGROUND: Lyme disease is a common vector-borne disease

caused

> by

> > the

> > > spirochete Borrelia burgdorferi (Bb), which manifests as

systemic

> > and

> > > targeted tissue inflammation. Both in vitro and in vivo studies

> have

> > > shown that Bb-induced inflammation is primarily host-mediated,

via

> > > cytokine or chemokine production that promotes leukocyte

> > > adhesion/migration. Whether Bb produces mediators that can

> directly

> > > alter the vascular permeability in vivo has not been

> investigated.

> > The

> > > objective of the present study was to investigate if Bb

produces a

> > > mediator(s) that can directly activate endothelial cells

> resulting

> > in

> > > increases in permeability in intact microvessels in the absence

> of

> > blood

> > > cells. METHODOLOGY/PRINCIPAL FINDINGS: The effects of cell-

free,

> > spent

> > > culture medium from virulent (B31-A3) and avirulent (B31-A) B.

> > > burgdorferi on microvessel permeability and endothelial calcium

> > > concentration, [Ca(2+)](i), were examined in individually

> perfused

> > rat

> > > mesenteric venules. Microvessel permeability was determined by

> > measuring

> > > hydraulic conductivity (Lp). Endothelial [Ca(2+)](i), a

necessary

> > signal

> > > initiating hyperpermeability, was measured in Fura-2 loaded

> > > microvessels. B31-A3 spent medium caused a rapid and transient

> > increase

> > > in Lp and endothelial [Ca(2+)](i). Within 2-5 min, the mean

peak

> Lp

> > > increased to 5.6+/-0.9 times the control, and endothelial [Ca

(2+)]

> > (i)

> > > increased from 113+/-11 nM to a mean peak value of 324+/-35 nM.

In

> > > contrast, neither endothelial [Ca(2+)](i) nor Lp was altered by

> B31-

> > A

> > > spent medium. CONCLUSIONS/SIGNIFICANCE: A mediator(s) produced

by

> > > virulent Bb under culture conditions directly activates

> endothelial

> > > cells, resulting in increases in microvessel permeability. Most

> > > importantly, the production of this mediator is associated with

Bb

> > > virulence and is likely produced by one or more of the 8 plasmid

> (s)

> > > missing from strain B31-A. PMID: 19116656

> > >

> >

>