Guest guest Posted May 28, 2006 Report Share Posted May 28, 2006 A titer chage from 300 to 600 might seem alot, but these may be antigen agglutination reactions done in 2-fold dilutions. You might have a series of reaction wells like this 1 1/2 1/4 1/8 1/16 1/32 1/64 1/128 1/256 1/512 1/1024 where 1 is undiluted. In class we looked at some of these. Once the antibody in your serum is diulted past a certain point, it is too dilute to agglutinate added the antigen (fixed amount per well) that is added to the wells. Typically there will be wells with clear agglutination, then a couple of sort of 'hazy' wells, followed by the more diluted wells that have no agglutination. So the difference between 1/300 and 1/600 titers may not be all that reproducable... ie may be within the error margin of the measurement. But I'm not certain that it is - I could be wrong. I'm also not 100% certain that 2-fold dilutions are typical or that agglutination assays are even typically used for this, but I think so. > > Have not read that. I did find that a simple 6 v battery produced > better lab results (in rising titers) than the Hulda e zapper. Quote Link to comment Share on other sites More sharing options...
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