Re: Use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience.

[Guest guest]

This group consistantly puts out great stuff.

Maybe they're perfect it so that someday it'll be mainstream testing.

Who funds these guys?

Too bad they didn't track down the blood sample's owners to see how

they're doing - healthy or not?

Barb

>

> http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?

cmd=Retrieve & db=pubmed & dopt=Abstract & list_uids=16207953 & query_hl=1

>

> J Clin Microbiol. 2005 Oct;43(10):4993-5002. Related

Articles, Links

>

>

> Use of shell-vial cell culture assay for isolation of bacteria from

clinical specimens: 13 years of experience.

>

> Gouriet F, Fenollar F, Patrice JY, Drancourt M, Raoult D.

>

> Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine,

Universite de la Mediterranee, 27 Boulevard Moulin, 13385

Marseille cedex 05, France.

>

> The shell-vial culture assay is performed routinely in our

laboratory. Recently we revisited our experience of using the shell-

vial culture assay for the isolation of microorganisms from various

clinical samples. Over a 13-year period, we have isolated 580

bacterial strains (5%) from 11,083 clinical samples tested. Over the

same period, 285 isolates of rickettsiae, bartonellae, or iella

burnetii were cultured from a total of 7,102 samples tested. These

isolates include 55 Rickettsia sp. isolates, 95 iella burnetii

isolates, and 135 Bartonella sp. isolates. Based on our experience

with the growth of fastidious microorganisms, we have used a

centrifugation shell-vial technique called JNSP, for " je ne sais pas "

( " I don't know [what I am growing] " ) for the isolation of other

microorganisms. A total of 173 isolates were cultured from the 3,861

clinical samples tested using the JNSP method. Of these, 40 isolates

had not been grown before on usual axenic medium. These include 2

Staphylococcus aureus isolates, 7 isolates of Streptococcus sp. and

related genera, 6 Mycobacterium sp. isolates, 1 Nocardia asteroides

isolate, 1 Actinomyces sp. isolate, 1 Brucella melitensis isolate, 2

Francisella tularensis isolates, 1 Mycoplasma pneumoniae isolate, and

1 Legionella pneumophila isolate. Using this protocol, we have also

cultured intracellular bacteria such as Chlamydia trachomatis and we

have performed the first culture and establishment of Trophyrema

whipplei. Applied in our laboratory, the shell-vial culture generally

exhibits a low rate of success. However, in some cases, this

technique allowed microbial diagnosis when classical agar procedure

and PCR were negative.

>

> PMID: 16207953 [PubMed - in process]

>

[Guest guest]

Barb,

Who funds them: I DO! Me and my fellow country-people, we fund them and they spit in our faces if we ask them to do any testing on us, real-life sick people with these complex infectious illnesses that they describe so well but which they refuse to deal with in real life, they don't want to be bothered with real people presenting with the very symptoms they are EXPLAINING so well, or at least explaining so well that these fastidious bugs cause chronic illness and yet cannot be easily identified.

They make me furious! Frothing at the mouth, in fact. My ID doc (who's a professor) who tries to actually deal with us poor sods with these infections, got told by Raoult that he was "treating nutcases" (us, me) almost as if the cardiac valves he tests were not attached to real people!

Mentally sane patients do not demand treatment for the kind of infectious illnesses he is constantly publishing about, it's almost like he is writing fiction and he is flabbergasted that people think Sherlock Holmes is a real person (if you see what I mean!)

Nelly

[infections] Re: Use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience.

This group consistantly puts out great stuff.Maybe they're perfect it so that someday it'll be mainstream testing.Who funds these guys?Too bad they didn't track down the blood sample's owners to see how they're doing - healthy or not?Barb>> http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve & db=pubmed & dopt=Abstract & list_uids=16207953 & query_hl=1> > J Clin Microbiol. 2005 Oct;43(10):4993-5002. Related Articles, Links > > > Use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience.> > Gouriet F, Fenollar F, Patrice JY, Drancourt M, Raoult D.> > Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Moulin, 13385 Marseille cedex 05, France.> > The shell-vial culture assay is performed routinely in our laboratory. Recently we revisited our experience of using the shell-vial culture assay for the isolation of microorganisms from various clinical samples. Over a 13-year period, we have isolated 580 bacterial strains (5%) from 11,083 clinical samples tested. Over the same period, 285 isolates of rickettsiae, bartonellae, or iella burnetii were cultured from a total of 7,102 samples tested. These isolates include 55 Rickettsia sp. isolates, 95 iella burnetii isolates, and 135 Bartonella sp. isolates. Based on our experience with the growth of fastidious microorganisms, we have used a centrifugation shell-vial technique called JNSP, for "je ne sais pas" ("I don't know [what I am growing]") for the isolation of other microorganisms. A total of 173 isolates were cultured from the 3,861 clinical samples tested using the JNSP method. Of these, 40 isolates had not been grown before on usual axenic medium. These include 2 Staphylococcus aureus isolates, 7 isolates of Streptococcus sp. and related genera, 6 Mycobacterium sp. isolates, 1 Nocardia asteroides isolate, 1 Actinomyces sp. isolate, 1 Brucella melitensis isolate, 2 Francisella tularensis isolates, 1 Mycoplasma pneumoniae isolate, and 1 Legionella pneumophila isolate. Using this protocol, we have also cultured intracellular bacteria such as Chlamydia trachomatis and we have performed the first culture and establishment of Trophyrema whipplei. Applied in our laboratory, the shell-vial culture generally exhibits a low rate of success. However, in some cases, this technique allowed microbial diagnosis when classical agar procedure and PCR were negative.> > PMID: 16207953 [PubMed - in process]>