RE: (Spirochete load) Where are they??

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It's no coincidence that Dr Cantwell finds CWD bacteria in sweat [glands] ...the mode of infection is via the lymphatic system ...that's where to look for the beasts ..

-----Original Message-----From: infections [mailto:infections ]On Behalf Of jill1313Sent: 17 October 2005 20:08infections Subject: [infections] Re: holy crap! qPCR of EM! (Spirochete load)SInce it avidly adheres to collagen matrices we should look there. But who knows what form it is in. Maybe in living tissue in vivo there are active spirochetes at various places but they are probably only one stage of growth. This bug is not well understood.> >> > > > > As far as why chetes aren't seen by microscopy - > > > > Yes, this was my main interest in this data... where are all those > > genomes coming from? Is it possible they are all undegraded genomes > > of dead cells? Or are some of them alive, composing the "missing > > biomass" that seems to be hidden somewhere when you dig the degree > of > > inflammation?> > > > > are they using TEM > > > or SEM for the microscopy (I haven't read the paper you're > > referring > > > to). You know even those these suckers can get to 20 microns > long - > > > some of them are only 100 nanometers wide, thats 0.1 microns > wide > > > so they AREN'T easy to see.> > > > High-performance immuno-optical (Aberer) and immuno-TEM (Hulinska). > I > > would expect detection of known Bb forms to be high, tho I dont > know > > of control data.> > > > > while we're on the size topic..> > > Picture THIS....> > > If I remember correctly an IgG un complexed antibody is 14 > > nanometes > > > in diameter that's only 0.014 microns- so picture a couple of > > > ntibodies have to be on a 20 micron long spirochete 100 naometer > > wide > > > spirochete - and remember the antibodies going to be attracted > to > > > only to specific protein segments..... probably looks like mice > > > running up a tree trunk.> > > > There are metal beads conjugated to the antibodies used in IEM. I > > think they are usually about 40 nm in size. The beads are black > > (electron-opaque) and perfectly circular, so they are very easy to > > see at 30,000x. If everything is done right and goes well, this > > should make it possible to visualize any bacterium including any > > putative tiny CWD under 0.1 um and smaller... if they have the > right > > antigens. > > > > > (And I think there is sometimg like 2000 antibodies mg/dl of > serum.> > > > > > It's a whole nuther world in there when you get it in perspective.> > > We just don't have all the technology to see whats happening in > > that > > > tiny world.> > > > I think we do have it. If antigen is there, and the Ab is > > complementary, and everyhting works well, the Ab should bind. It > > seems like a "needle in the haystack" affair for the complementary > > molecules to find one another... and it is - but organisms run on > > such events, they are the soul of biology. THe ferociously rapid > > motion of molecules is why it doesnt take eternity for > complementary > > moleculs to bind. Proteins vibrate, and water molecules switch > their > > intermolecular hydrogen bonds, at something like 10 billion times > per > > second. Looking down there from a human timescale of seconds and > > hours, you could say that every molecule in a solution is > effectively > > in contact with every molecule, all at once - tho that obviously is > > not the case at any given instant.> >>