Guest guest Posted October 17, 2005 Report Share Posted October 17, 2005 I sooo looked for this before, but didnt find it cause I used the alternative term qtPCR. This is of great importance for understanding lyme. " For those specimens in which B. burgdorferi was detectable by qPCR, the mean ± standard deviation number of spirochetes present in a 2- mm skin biopsy specimen was 2,462 ± 2,942, the median number of organisms was 1,450, and the number of spirochetes in positive specimens varied over 3 orders of magnitude (Fig. 1). " Why are 1/2 of such biopsies negative for Bb by microscopy, the rest showing only a few organisms (see Hulinska, see Aberer)? This may be hefty evidence that most borrelia are suboptical in size and do not express OspA, the common antigen used in IEM. There are other possible interpretations, such as lots of dead organisms with intact DNA... which seems unlikely to me. http://www.pubmedcentral.gov/articlerender.fcgi? tool=pubmed & pubmedid=11923340 Quote Link to comment Share on other sites More sharing options...
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