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resistance of Bb variants

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Nelly I've spent some time looking for work showing non-helical Bb

forms to be resistant, but havent been able to locate it.

Theres that one by (I think) Aberer showing that more cysts etc

formed in vitro in response to ceftriaxone (as opposed to non-B-

lactam drugs), but that experiment was only 2 days long. I dont think

it was made clear whether or not the ongoing presence of ceftriaxone

would have prevented those variant forms from multiplying, or whether

any forms would be able to multiply in the presence of tetracyclines,

etc.

The Jemsek Clinic treatment guidelines are referenced, yet they do

not reference their statement that the non-helical forms have broad

intrinsic resistance.

Klempner famously showed that the presence of host cells protected Bb

from eradication by high-dose ceftriaxone in vitro. But the mechanism

for that was not investigated, and other workers found that protein-

synth-inhibiting drugs did eradicate Bb from some sort of host cell

culture.

I looked for refs on this idea in some of the Brorson papers but cant

recall whether I finished. Anyways I didnt find anything so far.

So basically I havent seen anything showing that transformation into

nonhelical forms averts bacteriostasis, ie allows ongoing

reproduction in the presence of abx. If you or anyone can tip me off

about any such experiment I'd head to the library extremely

immediately.

I havent got around to significant study on the sequestered

anatomical sites problem yet, but hope to someday.

> ,

>

> Bb Cysts, blebs, granules? The Brorsons' studies etc Plenty to show

that spiros do have mechanisms to ensure survival under " adverse

circumstances " ie in the presence of most abx in-vitro. They can then

be " reborn " from their mutated forms.

>

> In-vivo they also " flagelate " their way to sequestered sites very

quickly and from there they can re-circulate and repopulate whenever

they choose to.

>

> But of course that's only hearsay, since me, myself, personally

have not got my own equipment in my backroom where I can play

microbiologist and then scream at people that what " I don't see(or

don't know enough about!) can't " possably " (sic) make you sick " .

>

> Nelly

>

>

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