Re: Tony. Barb, howell-jolly vs cocci

[Guest guest]

The pics go back a few years. REMEMBR Though the cfs patient has a

certain percentage of his red cells parasitized. What you do for

this is make a nice blood smear and count how many red cells and

what percent are parasitized.In other words if I'm looking in my

scope in a part of a blood smear that's got clean distribution of

cells as opposed to overlapping and clumped regions- I then make the

GUESSTIMATION of a percentage parasitized by doing a count.The

beauty is you drip a few drops of blood on agar plates and then you

have your culprits after incubation they grow out and reveala there

true selves and they also reveal there haemolytic potential.These

blood pathogens don't qualify for angel bacteria status. They have

all the dirty tricks of disease causing bacteria not contaminants.

Believe me I know what I would like to have floating all over and

throughout my body I got a sample recently of a species that is like

lactobacillus.

> Could you possibly get those bastards to multiply in a tube blood

> culture, to show they are cocci rather than howell-jollys? Maybe

one

> could incubate them 10h or so at 37 C, then compare with a fresh

blood

> sample?

>

> Contamination with non-target organisms would be one potential

> problem, and youd just have to do your best to ID things

> morphologically and with staining charecteristics.

>

> Another problem is that immune factors in blood in a tube might

check

> down the bugs just as well as blood does in your body, therefore

the

> bugs in the incubated blood might show no net growth. The

eucaryote

> protein synthesis inhibitor cycloheximide (beware, toxic) would

> probably shut down the leucocytes in the tube culture (tho it

would

> not effect existing complement or antibody in the blood). This

might

> (?) be enough to promote net multiplication of the bugs.

>

> Barb, can Howell-Jollys be way over at the edge of the red cell

like

> in that pic?

>

> Tony, was the pic prior to any treatment?

[Guest guest]

the affected area doesn't have to be in the center.

http://www.hematologica.pl/Atlas3/Angielska/Erythropoiesis/51e.htm

> Could you possibly get those bastards to multiply in a tube blood

> culture, to show they are cocci rather than howell-jollys? Maybe

one

> could incubate them 10h or so at 37 C, then compare with a fresh

blood

> sample?

>

> Contamination with non-target organisms would be one potential

> problem, and youd just have to do your best to ID things

> morphologically and with staining charecteristics.

>

> Another problem is that immune factors in blood in a tube might

check

> down the bugs just as well as blood does in your body, therefore

the

> bugs in the incubated blood might show no net growth. The eucaryote

> protein synthesis inhibitor cycloheximide (beware, toxic) would

> probably shut down the leucocytes in the tube culture (tho it would

> not effect existing complement or antibody in the blood). This might

> (?) be enough to promote net multiplication of the bugs.

>

> Barb, can Howell-Jollys be way over at the edge of the red cell

like

> in that pic?

>

> Tony, was the pic prior to any treatment?

[Guest guest]

COCCI describes a bacteria's SHAPE... it means it's round.

Bacilli are rods...

Cocci can be as small as 0.5 microns (um) or many times that large.

A red blood cell is about 7 .0 um in diameter.

TONY/ERIC......... if one had access to the right eqipment -

you wouldn't have to culture it.. if the sample was prepared

correctly, one could use a SEM (scanning electron microscope - which

are as big as a Buick) to ID.

See these pictures of a Target Cell.

http://www.vet.uga.edu/vpp/clerk/Boutureira/

> > Could you possibly get those bastards to multiply in a tube blood

> > culture, to show they are cocci rather than howell-jollys? Maybe

> one

> > could incubate them 10h or so at 37 C, then compare with a fresh

> blood

> > sample?

> >

> > Contamination with non-target organisms would be one potential

> > problem, and youd just have to do your best to ID things

> > morphologically and with staining charecteristics.

> >

> > Another problem is that immune factors in blood in a tube might

> check

> > down the bugs just as well as blood does in your body, therefore

> the

> > bugs in the incubated blood might show no net growth. The

> eucaryote

> > protein synthesis inhibitor cycloheximide (beware, toxic) would

> > probably shut down the leucocytes in the tube culture (tho it

> would

> > not effect existing complement or antibody in the blood). This

> might

> > (?) be enough to promote net multiplication of the bugs.

> >

> > Barb, can Howell-Jollys be way over at the edge of the red cell

> like

> > in that pic?

> >

> > Tony, was the pic prior to any treatment?