whole blood makes ~10x lesser TNF, IFNg response to LPS, dead bugs

August 23, 2005

FOr comparison.

The title says " impaired " but what they mean is " reduced. " After all

most of these people would probably die if they didnt aquire some

hefty tolerance to LPS etc during sepsis.

=============================

Shock. 2003 Feb;19(2):113-6. Related Articles, Links

Impaired production of interferon-gamma and tumor necrosis factor-

alpha but not of interleukin 10 in whole blood of patients with

sepsis.

Rigato O, Salomao R.

Division of Infectious Diseases, Escola ista de Medicina,

Universidade Federal de Sao o, Universiolade Federal de Sao

o, CEP 04039-032, Sao o, SP, Brazil.

It has been demonstrated that lipopolysaccharide (LPS)-induced

cytokine response in patients with sepsis differ from the normal

host, yet this has not been controlled for the presence of underlying

disease. We studied the ability of LPS and killed gram-negative

bacteria (GNB) to induce tumor necrosis factor (TNF)-alpha and

interleukin (IL) 10, and of phytohemagglutinin (PHA) to induce

interferon (IFN)-gamma, in whole blood from patients with sepsis (SP,

n = 20), patients with matched underlying disease and without sepsis

(control patients, n = 20), and healthy volunteers (HV, n = 20). LPS-

induced TNF-alpha production was lower in SP (median = 638 pg/mL)

compared with control patients (4060 pg/mL; P= 0.003), and control

patients production was lower compared with HV (5329 pg/mL; P <

0.001). Pseudomonas aeruginosa-induced TNF-alpha production was lower

in SP (1443 pg/mL) than in control patients (7319 pg/mL; P < 0.05),

and was not different between control patients and HV (6612 pg/mL; P

= 0.6). IFNy production was lower in SP (948 pg/mL) compared with

control patients (5516 pg/mL; P < 0.001), and the control patients

production was lower compared with HV (11,282 pg/mL; P < 0.001). IL-

10 production was not different among the three groups. Down-

regulation of TNF-alpha production in patients with sepsis, although

not restricted to them, was more pronounced with LPS than with GNB.

Although the presence of underlying disease may be involved in the

regulatory mechanisms of host response, the use of controls with

matched underlying diseases provides strong evidence for the septic

condition in the down-regulation of inflammatory response in patients

with sepsis.

PMID: 12578117 [PubMed - indexed for MEDLINE]

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