Jerry ... virus / borrelia interactions

[Guest guest]

Hi Jerry

Thought this one might interest you. I dont have easy access to the

full text.

(Unrelatedly, D Hulinska did a large share of the IEM work thats ever

been done on chronic lyme - found and refound it with species-

specific tags in patients receiving serious abx tx; most samples were

negative and the positives had very few organisms, but nearly the

same is true of erythema migrans using the same techniques.

He also discovered that cyst contents could be positive for OspA

while the cyst surface was negative.)

=========================================

Folia Biol (Praha). 2003;49(1):40-8. Related Articles, Links

Interaction of Borrelia burgdorferi sensu lato with Epstein-Barr

virus in lymphoblastoid cells.

Hulinska D, Roubalova K, Schramlova J.

National Reference Laboratory on Borreliosis, Electron Microscopy,

National Institute of Public Health, Prague, Czech Republic.

Since the possibility of interruption of latent EBV infection has

been suggested by the induction of the lytic virus cycle with

chemical substances, other viruses, and by immunosuppression, we

hypothesized that the same effect might happen in B. burgdorferi

sensu lato infection as happens in Lyme disease patients with

positive serology for both agents. We have observed EBV replication

in lymphoblastoid cells after superinfection with B. garinii and B.

afzelii strains after 1 and 4 h of their interaction. We found that

viral and borrelial antigens persisted in the lymphoblasts for 3 and

4 days. Morphological and functional transformation of both agents

facilitate their transfer to daughter cells. Association with

lymphoblasts and internalization of B. garinii by tube phagocytosis

increased replication of viruses more successfully than B. afzelii

and chemical inductors. Demonstration of such findings must be

interpreted cautiously, but may prove a mixed borrelial and viral

cause of severe neurological disease.

PMID: 12630667 [PubMed - indexed for MEDLINE]

[Guest guest]

(Unrelatedly, D Hulinska did a large share of the IEM work thats ever been done on chronic lyme - found and refound it with species-specific tags in patients receiving serious abx tx; most samples were negative and the positives had very few organisms, but nearly the same is true of erythema migrans using the same techniques. He also discovered that cyst contents could be positive for OspA while the cyst surface was negative.)

,

Not meaning to be pedantic but D Hulinska (and the others K Roubalova, and J Schramlova) are all women (you can tell by the ending of their surnames -ska not -ski for a man, -ova not ov/off for a man (conf Navratilova-definitely a lady!)

Nelly

=========================================Folia Biol (Praha). 2003;49(1):40-8. Related Articles, Links Interaction of Borrelia burgdorferi sensu lato with Epstein-Barr virus in lymphoblastoid cells.Hulinska D, Roubalova K, Schramlova J.National Reference Laboratory on Borreliosis, Electron Microscopy, National Institute of Public Health, Prague, Czech Republic.Since the possibility of interruption of latent EBV infection has been suggested by the induction of the lytic virus cycle with chemical substances, other viruses, and by immunosuppression, we hypothesized that the same effect might happen in B. burgdorferi sensu lato infection as happens in Lyme disease patients with positive serology for both agents. We have observed EBV replication in lymphoblastoid cells after superinfection with B. garinii and B. afzelii strains after 1 and 4 h of their interaction. We found that viral and borrelial antigens persisted in the lymphoblasts for 3 and 4 days. Morphological and functional transformation of both agents facilitate their transfer to daughter cells. Association with lymphoblasts and internalization of B. garinii by tube phagocytosis increased replication of viruses more successfully than B. afzelii and chemical inductors. Demonstration of such findings must be interpreted cautiously, but may prove a mixed borrelial and viral cause of severe neurological disease.PMID: 12630667 [PubMed - indexed for MEDLINE]