Re: Jill - Bb tissue culture model

[Guest guest]

YES!!! Thanks!!! Emailed it to myself.

Did I already ask on this list...I need one antibody to bind to

borrelia that is NOT OSP A. Osp C keeps changing so that's no good.

I'm going to call some people next week. Maybe there is none.

> I think you mightve been looking for this one once to show me, or

> maybe it was something else similar:

>

> PMID: 15838803

>

> Invasion of Human Tissue Ex Vivo by Borrelia burgdorferi

>

> H. Duray,1 Shu-Rong Yin,2 Yoshinori Ito,2 Ludmila Bezrukov,2

> Cheri ,2 Myong-Soon Cho,2 Fitzgerald,2 Dorward,3

> Zimmerberg,2 and Leonid Margolis2

>

> 1Department of Pathology, National Cancer Institute, National

> Institutes of Health, and 2National Aeronautics and Space

> Administration/National Institutes of Health Center for Three-

> Dimensional Tissue Culture, Laboratory of Cellular and Molecular

> Biophysics, National Institute of Child Health and Human

Development,

> National Institutes of Health, Bethesda, land; 3National

> Institute of Allergy and Infectious Diseases, National Institutes

of

> Health, Rocky Mountain Laboratories, Hamilton, Montana

>

>

>

> Borrelia burgdorferi sensu stricto is an etiological agent of Lyme

> disease. The lack of an adequate ex vivo system for human tissue

> infection is an obstacle to fully understanding the molecular

> mechanisms of invasion of tissue by B. burgdorferi and its

adaptation

> within the human host. Here, we report on the development of such a

> system. We inoculated blocks of human tonsillar tissue with B.

> burgdorferi spirochetes, cultured them in a low-shear rotating wall

> vessel (RWV) bioreactor, and analyzed them using light and electron

> microscopy, nested polymerase chain reaction (PCR), and

quantitative

> real-time PCR. Also, we evaluated the expression of the outer

surface

> proteins (Osps) OspA and OspC by use of quantitative Western

> blotting. Light and electron microscopic analysis revealed multiple

> spirochetes localized extracellularly within the tissue, and their

> identity was confirmed by PCR. Quantification of spirochetes inside

> the RWV-cultured tonsillar tissue demonstrated that the number of

B.

> burgdorferi exceeded the initial inoculum by an order of magnitude,

> indicating that spirochetes replicated in the tissue. Electron

> microscopic analysis showed that some spirochetes were arranged in

> cystic structures and that invading spirochetes differentially

> expressed surface proteins; both of these features have been

> described for infected tissues in vivo. The system we have

developed

> can be used to study B. burgdorferi pathogenesis under controlled

> conditions ex vivo, in particular to explore the gene activation

> responsible for the adaptation of B. burgdorferi to human tissue

that

> leads to Lyme disease.

[Guest guest]

Check that Cullen and Haake article on spiro membrane proteins. They

talk about alot of the vaccine target research. I think there may have

been one other that was kinda-sorta-invariant? I dont really remember.

Anyway its only 30 pages of dense molecular biology enjoyment.

Anyways I'm kinda swingin back towards thinking the bastards are

suboptical intracellular dots, myself.

> YES!!! Thanks!!! Emailed it to myself.

> Did I already ask on this list...I need one antibody to bind to

> borrelia that is NOT OSP A. Osp C keeps changing so that's no good.

> I'm going to call some people next week. Maybe there is none.

[Guest guest]

Where is that?

> > YES!!! Thanks!!! Emailed it to myself.

> > Did I already ask on this list...I need one antibody to bind to

> > borrelia that is NOT OSP A. Osp C keeps changing so that's no

good.

> > I'm going to call some people next week. Maybe there is none.