Re: Tony - wet mounts

[Guest guest]

Tarello seems to publish almost exclusively about animals. If you

make it to the library and have time, the full text of this one

might be fun to look at:

Acta Vet Hung. 2003;51(1):61-72. [Paragraph breaks added]

Immunological anomalies and thrombocytopenia in 117 dogs and cats

diagnosed with chronic fatigue syndrome (CFS).

Tarello W.

Clinica Veterinaria Airone of Nus, Aosta, Italy. wtarello@...

Retrospective analysis of immune dysfunctions found in 55 dogs and

62 cats diagnosed with Chronic Fatigue Syndrome (CFS), revealed

leukopenia in 11% of dogs (n = 6) and 22.5% of cats (n = 14),

lymphopenia in 14.5% of dogs (n = 8) and 10% of cats (n = 6),

hypogammaglobulinaemia in 9% of dogs (n = 5) and 13% of cats (n = 8)

and thrombocytopenia in 20% of dogs (n = 11) and 68% of cats (n =

42).

All patients had creatine kinase enzyme levels above the normal

range (CK = 5-100 IU/L) and carried micrococcus-like organisms on

erythrocytes. Blood cultures proved positive for Staphylococcus spp.

in 16 cases. After low-dosage arsenic-based therapy (thiacetarsamide

sodium) all animals experienced complete clinical remission.

Subsequent controls demonstrated immune restoration in 4

representative FIV-FeLV negative cats, previously diagnosed with CFS

associated with leukopenia, lymphopenia, hypogammaglobulinaemia and

thrombocytopenia.

The main conclusion is that a CFS-like disease in dogs and cats,

characterised by the common hallmarks of high CK levels, absence of

known causes of chronic fatigue in animals and presence of

micrococcus-like organisms in the blood, can be associated with

humoral and/or cellular immune deficiencies in 9-22.5% of cases and

with thrombocytopenia in 20-68% of cases. Considerations are made on

the possible role of micrococci in the aetiology of the condition

and on the similarities with CFS in humans.

PMID: 12688127 [PubMed - indexed for MEDLINE]

> What do you see in fresh thin blood smears? Have you tried one

with

> Geimsa or stains? Does your scope not quite have the res to

> visualize the Tarello cocci attached to red cells? It seems like

theres

> a very important question as to how many people show those cocci.

[Guest guest]

, here's one he published on humans ..himself along with his wife ..pass the arsenic please!!

Comp Immunol Microbiol Infect Dis. 2001 Oct;24(4):233-46.

Related Articles,

Links

Chronic fatigue syndrome (CFS) associated with Staphylococcus spp. bacteremia, responsive to potassium arsenite 0.5% in a veterinary surgeon and his coworking wife, handling with CFS animal cases.Tarello W.wtarello@...Chronic fatigue syndrome (CFS) in human patients remain a controversial and perplexing condition with emerging zoonotic aspects. Recent advances in human medicine seem to indicate a bacterial etiology and the condition has already been described in horses, dogs, cats and birds of prey in association with micrococci-like organisms in the blood. To evaluate the possibility of a chronic bacteremia, a veterinary surgeon (the author) and his coworking wife, both diagnosed with CFS and meeting the CDC working case definition, were submitted to rapid blood cultures and fresh blood smears investigations. Blood cultures proved Staph-positive and micrococci-like organisms in the blood were repeatedly observed in the 3-year period preceding the arsenical therapy, during which several medicaments, including antibiotics, proved unsuccessful. Following treatment with a low dosage arsenical drug (potassium arsenite 0.5%, im., 1 ml/12 h, for 10 days) both patients experienced complete remission. At the post-treatment control made 1 month later, micrococci had disappeared from the blood, and the CD4/CD8 ratio was raising.Publication Types:

Case ReportsPMID: 11561958 [PubMed - indexed for MEDLINE]

-----Original Message-----From: infections [mailto:infections ]On Behalf Of SchaafsmaSent: 26 July 2005 19:41infections Subject: [infections] Re: Tony - wet mountsTarello seems to publish almost exclusively about animals. If you make it to the library and have time, the full text of this one might be fun to look at:Acta Vet Hung. 2003;51(1):61-72. [Paragraph breaks added]Immunological anomalies and thrombocytopenia in 117 dogs and cats diagnosed with chronic fatigue syndrome (CFS).Tarello W.Clinica Veterinaria Airone of Nus, Aosta, Italy. wtarello@...Retrospective analysis of immune dysfunctions found in 55 dogs and 62 cats diagnosed with Chronic Fatigue Syndrome (CFS), revealed leukopenia in 11% of dogs (n = 6) and 22.5% of cats (n = 14), lymphopenia in 14.5% of dogs (n = 8) and 10% of cats (n = 6), hypogammaglobulinaemia in 9% of dogs (n = 5) and 13% of cats (n = 8) and thrombocytopenia in 20% of dogs (n = 11) and 68% of cats (n = 42). All patients had creatine kinase enzyme levels above the normal range (CK = 5-100 IU/L) and carried micrococcus-like organisms on erythrocytes. Blood cultures proved positive for Staphylococcus spp. in 16 cases. After low-dosage arsenic-based therapy (thiacetarsamide sodium) all animals experienced complete clinical remission. Subsequent controls demonstrated immune restoration in 4 representative FIV-FeLV negative cats, previously diagnosed with CFS associated with leukopenia, lymphopenia, hypogammaglobulinaemia and thrombocytopenia. The main conclusion is that a CFS-like disease in dogs and cats, characterised by the common hallmarks of high CK levels, absence of known causes of chronic fatigue in animals and presence of micrococcus-like organisms in the blood, can be associated with humoral and/or cellular immune deficiencies in 9-22.5% of cases and with thrombocytopenia in 20-68% of cases. Considerations are made on the possible role of micrococci in the aetiology of the condition and on the similarities with CFS in humans.PMID: 12688127 [PubMed - indexed for MEDLINE]> What do you see in fresh thin blood smears? Have you tried one with > Geimsa or stains? Does your scope not quite have the res to > visualize the Tarello cocci attached to red cells? It seems like theres > a very important question as to how many people show those cocci.

[Guest guest]

Yeah

My scopes pretty cool coming in at about 1500 US new- it's just below

the lab scopes.I have a diff quick stain which is a simple wright

stain. I also have a few other stains the giemsa may grumwold (sp and

if I got it right)is an alternative to this wright stain.

The reason I sent my slides to tarello is that your red blood cells

are damaged, have spikes, and stuff attached that needed proper

interpretation, as first time off the blocks I needed

guidance.Actually I was impressed with tarello and his percentage of

red cell adhesion. I think most people on the planet can run foul with

there colonising bacteria and if you have 10 % or 20% attached

bacteria to your red cells it would be interesting to find this

out.Basically many people on the planet are in the JUST FUNCTIONING

MODE, we just got tipped over the edge.

Also tarello has a scope that can take pictures. I also did mine not

wet mount but within minutes of drying it was stained.You really have

to have a thin smear because a lot of clumping does show up in most of

the slide- you need to get to the thin area's to make your

observations.

What pisses me of is that people don't realise labs don't look at

blood under the 400 resolution.At 1000X oil immersion you possably get

too much info to process and you can possably get bad results on so

called normal folk, it's that fine line interpretation that needs

initial processing for us amateurs.

tony

> What do you see in fresh thin blood smears? Have you tried one with

> Geimsa or stains? Does your scope not quite have the res to

> visualize the Tarello cocci attached to red cells? It seems like

theres

> a very important question as to how many people show those cocci.