Guest guest Posted July 24, 2005 Report Share Posted July 24, 2005 http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed & pubmedid=15984913 PLoS Biol. 2005 Jul 5;3(8):e248 [Epub ahead of print] Related Articles, Links The Genome Sequence of Rickettsia felis Identifies the First Putative Conjugative Plasmid in an Obligate Intracellular Parasite.Ogata H, Renesto P, Audic S, C, Blanc G, Fournier PE, Parinello H, Claverie JM, Raoult D.Structural and Genomic Information Laboratory, UPR 2589, IBSM, CNRS, Marseille Cedex, France.We sequenced the genome of Rickettsia felis, a flea-associated obligate intracellular alpha-proteobacterium causing spotted fever in humans. Besides a circular chromosome of 1,485,148 bp, R. felis exhibits the first putative conjugative plasmid identified among obligate intracellular bacteria. This plasmid is found in a short (39,263 bp) and a long (62,829 bp) form. R. felis contrasts with previously sequenced Rickettsia in terms of many other features, including a number of transposases, several chromosomal toxin-antitoxin genes, many more spoT genes, and a very large number of ankyrin- and tetratricopeptide-motif-containing genes. Host-invasion-related genes for patatin and RickA were found. Several phenotypes predicted from genome analysis were experimentally tested: conjugative pili and mating were observed, as well as beta-lactamase activity, actin-polymerization-driven mobility, and hemolytic properties. Our study demonstrates that complete genome sequencing is the fastest approach to reveal phenotypic characters of recently cultured obligate intracellular bacteria.PMID: 15984913 [PubMed - as supplied by publisher] Quote Link to comment Share on other sites More sharing options...
Recommended Posts
Join the conversation
You are posting as a guest. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.