Re: Bbsl prevalence in Missouri, USA

[Guest guest]

Okay, doesn't this last paragraph seem kind of significant?

Actually, kind of huge???

" Five of the Missouri isolates were obtained from the Dowd farm which

is the site of a human patient with a classical erythema migrans (EM)

lesion and clinically diagnosed with LD. Interestingly, however,

preliminary experiments involving needle injection of 2 of the

isolates into mice failed to infect the mice. "

Why would injecting the isolates not infect the mice? Does this mean

we're dealing with an immune system problem first, not a contagion

problem?

Does anybody know if IGENEX has done any trials with healthy

patients as controls? Do we know whether the same number of healthy

people tested positive on their tests? (I remember a friend telling

me that the founder of Igenex told her something along these lines).

That would mean the question is why can't some of us recover from

these organisms? Or why do we get overwhelmed by them when others

don't? It's the same with staph. Everyone carries staph. But why do

we have billions times more of the critters than other people?

That's why even though I'm sick, I don't make other people sick,

unless they are also predisposed or some how exposed to the same

conditions I have making us more susceptible.

penny

> This is a crosspost from lymenet. Wherever its from, its not in

> pubmed yet. Whole text from lymenet is below, but I'll spare ya

the

> suspense:

>

> " Western blots are being conducted to confirm positive ELISA

results.

> Rabbits, deer, white-footed mice, cotton mice, and deer all show a

> prevalence of 65% or more positive for presence of IgG antibodies

to

> the MOD-1 Bb isolate. "

>

>

> ================================================================

>

>

> H. Oliver, Jr., PhD

> Institute of Arthropodology & Parisitology, Georgia Southern

> University

> Bb sensu lato Isolates from Missouri

> J.H. Oliver, Jr., T.M. Kollars, Jr., and F.W. Chandler, Jr.

Institute

> of Arthropodology and Parasitology, Georgia Southern University

>

> A total of 31 species of vertebrate animals were examined for

> presence of ticks and spirochetes at 20 study sites in

southeastern

> Missouri.

>

> These included 22 mammals, 6 birds, and 3 reptile species. Ticks

were

> also obtained by flagging/dragging the vegetation. Tick and/or

host

> tissues were inoculated into BSKII medium in hopes of establishing

> laboratory cultures of spirochetes.

>

> A total of 45 isolates were obtained from ticks attached to 15

> eastern cottontail rabbits from 8 sites located in 5 counties.

>

> All isolates were from various developmental stages of Ixodes

> dentatus except one was from an Amblyomma americanum larva and one

> from a Haemaphysalis leporispalustris larva. The spirochetes were

> routinely screened by IFA monoclonal antibodies specific for Bb

and

> included OspA (H5332, H3TS), OspB (H5TS,H6831, H614), and the

> Borrelia genus-specific H9724; two B. hermsii specific antibodies

> (9826, H4825) were also tested.

>

> The spirochete isolates were also analyzed by the PCR using

several

> Bb specific ospA primers (788/944, 149/319, 149/459,3'/5'), fla

> (245/855), and 's chromosomal primer (147/520). Several of the

> isolates were subjected to SDS-PAGE analysis and pulsed-field gel

> electrophoresis (PFGE).

>

> Based on the above analyses all of the Missouri spirochete

isolates

> are considered to be Bb sensu lato but are phenotypically and

> genetically different from Bb sensu stricto and in fact, appear to

be

> rather similar to the genospecies B. andersonii.

>

> There are interesting differences among the Missouri isolates

> themselves. There are at least 5 immunological types and a

dendrogram

> based on those data including 5 isolates from the Dowd farm and B.

> burgdorferi s.s., B. garinii, and B. hermsii suggests that MOD-5

is

> more closely related to B-31 B. burgdorferi s.s. than to MOD-1,

MOD-

> 2, MOD-3, and MOD-6.

>

> This relationship appears to be similar when a dendrogram is

> constructed based on data from pulsed-field gel electrophoresis.

> There are also at least 5 PCR types among the Missouri isolates

thus

> far screened.

>

> An ELISA for detecting antibodies against Missouri isolate MOD-1

was

> developed and analyses of sera from mammals collected in southeast

> Missouri thus far indicate high prevalences of antibodies in

several

> species of hosts of ticks with the highest in cottontail rabbits

> followed by white-tailed deer, rodents, and raccoons.

>

> Western blots are being conducted to confirm positive ELISA

results.

> Rabbits, deer, white-footed mice, cotton mice, and deer all show a

> prevalence of 65% or more positive for presence of IgG antibodies

to

> the MOD-1 Bb isolate.

>

> Five of the Missouri isolates were obtained from the Dowd farm

which

> is the site of a human patient with a classical erythema migrans

(EM)

> lesion and clinically diagnosed with LD. Interestingly, however,

> preliminary experiments involving needle injection of 2 of the

> isolates into mice failed to infect the mice.