Guest guest Posted April 30, 2005 Report Share Posted April 30, 2005 Theres a variety of matrix proteins out in the extracellular matrix, that hold you together. I think these are partially degraded in inflammation. Also, Nelly had a paper a little while ago that I think showed how Bb manipulates the hosts secretion of these enzymes, in order to degrade the brain epithelium and enter the brain. > Can you refresh me what these do in lyme? (metalloproteinases or > whatever those things are) > > I just listened to a lecture that included them as making blood > vessels leaky, and inflammation and so on. I remember they are > important in lyme. Thanx. Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 30, 2005 Report Share Posted April 30, 2005 I found what I needed. I will reproduce it here. I wanted to know because Trent Nichols MD uses the comitras (Natcell/Atrium Biotech) for his lyme becuase it downregulates the MMP's. And he finds it very helpful and its VERYYYYYYYY expensive. $375/mo supply. I asked him about 3 times, is he sure this is helping because its so expensive, and he said yes. But I'm willing to try it, I think. Have to look hard at my finances. But I feel where my health is concerned that comes first. Well, shelter and food come first. Here is the article: Infection and Immunity, January 2005, p. 126-134, Vol. 73, No. 1 0019-9567/05/$08.00+0 doi:10.1128/IAI.73.1.126-134.2005 Copyright © 2005, American Society for Microbiology. All Rights Reserved. Induction of Host Matrix Metalloproteinases by Borrelia burgdorferi Differs in Human and Murine Lyme Arthritis Aruna K. Behera,1 Ethan Hildebrand,1 Joanna Scagliotti,1 C. Steere,2 and Linden T. Hu1* Department of Infectious Disease, Tupper Research Institute, New England Medical Center, Tufts University School of Medicine,1 Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts2 Received 13 September 2004/ Accepted 17 September 2004 Matrix metalloproteinases (MMPs) are induced from host tissues in response to Borrelia burgdorferi. Upregulation of MMPs may play a role in the dissemination of the organism through extracellular matrix tissues, but it can also result in destructive pathology. Although mice are a well-accepted model for Lyme arthritis, there are significant differences compared to human disease. We sought to determine whether MMP expression could account for some of these differences. MMP expression patterns following B. burgdorferi infection were analyzed in primary human chondrocytes, synovial fluid samples from patients with Lyme arthritis, and cartilage tissue from Lyme arthritis-susceptible and -resistant mice by using a gene array, real-time PCR, an enzyme-linked immunosorbent assay, and immunohistochemistry. B. burgdorferi infection significantly induced transcription of MMP-1, -3, -13, and -19 from primary human chondrocyte cells. Transcription of MMP-10 and tissue inhibitor of metalloprotease 1 was increased with B. burgdorferi infection, but protein expression was only minimally increased. The synovial fluid levels of MMPs from patients with high and low spirochete burdens were consistent with results seen in the in vitro studies. B. burgdorferi-susceptible C3H/HeN mice infected with B. burgdorferi showed induction of MMP-3 and MMP-19 but no other MMP or tissue inhibitor of metalloprotease. As determined by immunohistochemistry, MMP-3 expression was increased only in chondrocytes near the articular surface. The levels of MMPs were significantly lower in the more Lyme arthritis-resistant BALB/c and C57BL/6 mice. Differences between human and murine Lyme arthritis may be related to the lack of induction of collagenases, such MMP-1 and MMP-13, in mouse joints Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 30, 2005 Report Share Posted April 30, 2005 Jill - You mention Trent Nichols, who is very close to where I live - do you live near him also? If so, would sure like to know- would be nice to know if you live near me. Also, do you see him and, if so , what is his treatment program like? Thank you - Les [infections] Re: MMP's....eric et al I found what I needed. I will reproduce it here. I wanted to know because Trent Nichols MD uses the comitras (Natcell/Atrium Biotech) for his lyme becuase it downregulates the MMP's. And he finds it very helpful and its VERYYYYYYYY expensive. $375/mo supply. I asked him about 3 times, is he sure this is helping because its so expensive, and he said yes.But I'm willing to try it, I think. Have to look hard at my finances. But I feel where my health is concerned that comes first. Well, shelter and food come first.Here is the article:Infection and Immunity, January 2005, p. 126-134, Vol. 73, No. 10019-9567/05/$08.00+0 doi:10.1128/IAI.73.1.126-134.2005Copyright © 2005, American Society for Microbiology. . Induction of Host Matrix Metalloproteinases by Borrelia burgdorferi Differs in Human and Murine Lyme Arthritis Aruna K. Behera,1 Ethan Hildebrand,1 Joanna Scagliotti,1 C. Steere,2 and Linden T. Hu1* Department of Infectious Disease, Tupper Research Institute, New England Medical Center, Tufts University School of Medicine,1 Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts2 Received 13 September 2004/ Accepted 17 September 2004 Matrix metalloproteinases (MMPs) are induced from host tissues in response to Borrelia burgdorferi. Upregulation of MMPs may play a role in the dissemination of the organism through extracellular matrix tissues, but it can also result in destructive pathology. Although mice are a well-accepted model for Lyme arthritis, there are significant differences compared to human disease. We sought to determine whether MMP expression could account for some of these differences. MMP expression patterns following B. burgdorferi infection were analyzed in primary human chondrocytes, synovial fluid samples from patients with Lyme arthritis, and cartilage tissue from Lyme arthritis-susceptible and -resistant mice by using a gene array, real-time PCR, an enzyme-linked immunosorbent assay, and immunohistochemistry. B. burgdorferi infection significantly induced transcription of MMP-1, -3, -13, and -19 from primary human chondrocyte cells. Transcription of MMP-10 and tissue inhibitor of metalloprotease 1 was increased with B. burgdorferi infection, but protein expression was only minimally increased. The synovial fluid levels of MMPs from patients with high and low spirochete burdens were consistent with results seen in the in vitro studies. B. burgdorferi-susceptible C3H/HeN mice infected with B. burgdorferi showed induction of MMP-3 and MMP-19 but no other MMP or tissue inhibitor of metalloprotease. As determined by immunohistochemistry, MMP-3 expression was increased only in chondrocytes near the articular surface. The levels of MMPs were significantly lower in the more Lyme arthritis-resistant BALB/c and C57BL/6 mice. Differences between human and murine Lyme arthritis may be related to the lack of induction of collagenases, such MMP-1 and MMP-13, in mouse joints Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 30, 2005 Report Share Posted April 30, 2005 NO, I live in NY. I met him at a conference where I Talked to him for a few hours. If you live near him and can afford it, go to him. I don't know any of his patients but I do know from speaking with him for 2 hours that he is brilliant. > Jill - You mention Trent Nichols, who is very close to where I live - do you live near him also? If so, would sure like to know- would be nice to know if you live near me. > Also, do you see him and, if so , what is his treatment program like? Thank you - Les > ----- Original Message ----- > Quote Link to comment Share on other sites More sharing options...
Guest guest Posted April 30, 2005 Report Share Posted April 30, 2005 Thanks for the info on Dr Nichols - is always nice to know of a good doctor that is close by- Les [infections] Re: MMP's....eric et al NO, I live in NY. I met him at a conference where I Talked to him for a few hours. If you live near him and can afford it, go to him. I don't know any of his patients but I do know from speaking with him for 2 hours that he is brilliant. > Jill - You mention Trent Nichols, who is very close to where I live -do you live near him also? If so, would sure like to know- would be nice to know if you live near me. > Also, do you see him and, if so , what is his treatment program like? Thank you - Les> ----- Original Message ----- > Quote Link to comment Share on other sites More sharing options...
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