Re: MBP and endothelium auto=AB

[Guest guest]

Lorene: You can get MBP and brain antibodies done at Immunosciences in

Beverly Hills, CA but I don't know about getting it here from Edinburgh

(we'll be doing a miniDAN! at University of Edinburgh in May, BTW). I'm not

sure what " endothelium proteins " refers to, or what " retalted " means -

please enlighten! Dr. JM

MBP and endothelium auto=AB

> Dear Listmates,

>

> Just trying to find out where we could have some auto-antibody against

> MBP and endothelium proteins (or other retalted molecules) measured?

> Any advice?

>

> Lorene/ Edinburgh

>

>

>

>

>

>

> Many frequently asked questions and answers can be found at

<http://forums.autism-rxguidebook.com/default.aspx>

>

>

[Guest guest]

Dear Jaquelyn,

Thanks for this; I was asking on behalf of another parent; I believe

what she meant comes from a study by Connolly et al, 1999, that shows

that sera from ASD children do react `'abnormally' to human cortex and

specifically to endothelial cells- The presence of IgM against

endothelial cells is also reported in this paper. And retalted means

related, ooops.

I am a Director of Action Against Autism, so I sure know that you'll

be coming next May in Edinburgh! I am really looking forward to this

and meet with Jack as well!

Thanks again for your wonderful support,

Lorene

Serum autoantibodies to brain in Landau-Kleffner variant, autism, and

other neurologic disorders.

Connolly AM, Chez MG, Pestronk A, Arnold ST, Mehta S, Deuel RK.

Departments of Neurology and Pediatrics, Washington University, St.

Louis Children's Hospital, St Louis, Missouri, USA.

OBJECTIVE: Etiologically unexplained disorders of language and social

development have often been reported to improve in patients treated

with immune-modulating regimens. Here we determined the frequency of

autoantibodies to brain among such children. DESIGN: We collected sera

from a cohort of children with (1) pure Landau-Kleffner syndrome (n =

2), (2) Landau-Kleffner syndrome variant (LKSV, n = 11), and (3)

autistic spectrum disorder (ASD, n = 11). None had received

immune-modulating treatment before the serum sample was obtained.

Control sera (n = 71) were from 29 healthy children, 22 with

non-neurologic illnesses (NNIs), and 20 children with other neurologic

disorders (ONDs). We identified brain autoantibodies by immunostaining

of human temporal cortex and antinuclear autoantibodies using

commercially available kits. RESULTS: IgG anti-brain autoantibodies

were present in 45% of sera from children with LKSV, 27% with ASD, and

10% with ONDs compared with 2% from healthy children and control

children with NNIs. IgM autoantibodies were present in 36% of sera

from children with ASD, 9% with LKSV, and 15% with ONDs compared with

0% of control sera. Labeling studies identified one antigenic target

to be endothelial cells. Antinuclear antibodies with titers >/=1:80

were more common in children with ASD and control children with ONDs.

CONCLUSION: Children with LKSV and ASD have a greater frequency of

serum antibodies to brain endothelial cells and to nuclei than

children with NNIs or healthy children. The presence of these

antibodies raises the possibility that autoimmunity plays a role in

the pathogenesis of language and social developmental abnormalities in

a subset of children with these disorders.

PMID: 10228297 [PubMed - indexed for MEDLINE]

Lorene: You can get MBP and brain antibodies done at Immunosciences in

Beverly Hills, CA but I don't know about getting it here from Edinburgh

(we'll be doing a miniDAN! at University of Edinburgh in May, BTW).

I'm not

sure what " endothelium proteins " refers to, or what " retalted " means -

please enlighten! Dr. JM

[Guest guest]

Connolly's team still may have the assay reagents on the shelf. If you,

you might want to contact Ms. Connolly. Last time I tried, her email was

findable in directory of Wash U in St Louie. ISL could do the assay if

they'd work on it for a while. The Connolly findings point to an

additional pathway whereby hypoperfusion can occur. Also, intramonocyte

pathogens can be evaluated, eg, by determining viral load in PBMCS,

especially in monocytes. Many labs have flow cytometers, but are not

current in the med lit regarding viral load determinations - except for

HIV and HepC, which (of course) act differently from CMV, HHV6, MV, and

other intramonocyte pathogens. At least the millennium is young, there's

hope at least one autism friendly lab will get around to the

aforementioned assays and the etiologic pathways that could be

identified in yet another subgroup or several.

Thnx for stressing the point that endothelial abs are NOT mbp abs.

greenbank2003 wrote:

>Dear Jaquelyn,

>

>Thanks for this; I was asking on behalf of another parent; I believe

>what she meant comes from a study by Connolly et al, 1999, that shows

>that sera from ASD children do react `'abnormally' to human cortex and

>specifically to endothelial cells- The presence of IgM against

>endothelial cells is also reported in this paper. And retalted means

>related, ooops.

>

>I am a Director of Action Against Autism, so I sure know that you'll

>be coming next May in Edinburgh! I am really looking forward to this

>and meet with Jack as well!

>

>Thanks again for your wonderful support,

>

>Lorene

>Serum autoantibodies to brain in Landau-Kleffner variant, autism, and

>other neurologic disorders.

>

>Connolly AM, Chez MG, Pestronk A, Arnold ST, Mehta S, Deuel RK.

>

>Departments of Neurology and Pediatrics, Washington University, St.

>Louis Children's Hospital, St Louis, Missouri, USA.

>

>OBJECTIVE: Etiologically unexplained disorders of language and social

>development have often been reported to improve in patients treated

>with immune-modulating regimens. Here we determined the frequency of

>autoantibodies to brain among such children. DESIGN: We collected sera

>from a cohort of children with (1) pure Landau-Kleffner syndrome (n =

>2), (2) Landau-Kleffner syndrome variant (LKSV, n = 11), and (3)

>autistic spectrum disorder (ASD, n = 11). None had received

>immune-modulating treatment before the serum sample was obtained.

>Control sera (n = 71) were from 29 healthy children, 22 with

>non-neurologic illnesses (NNIs), and 20 children with other neurologic

>disorders (ONDs). We identified brain autoantibodies by immunostaining

>of human temporal cortex and antinuclear autoantibodies using

>commercially available kits. RESULTS: IgG anti-brain autoantibodies

>were present in 45% of sera from children with LKSV, 27% with ASD, and

>10% with ONDs compared with 2% from healthy children and control

>children with NNIs. IgM autoantibodies were present in 36% of sera

>from children with ASD, 9% with LKSV, and 15% with ONDs compared with

>0% of control sera. Labeling studies identified one antigenic target

>to be endothelial cells. Antinuclear antibodies with titers >/=1:80

>were more common in children with ASD and control children with ONDs.

>CONCLUSION: Children with LKSV and ASD have a greater frequency of

>serum antibodies to brain endothelial cells and to nuclei than

>children with NNIs or healthy children. The presence of these

>antibodies raises the possibility that autoimmunity plays a role in

>the pathogenesis of language and social developmental abnormalities in

>a subset of children with these disorders.

>

>PMID: 10228297 [PubMed - indexed for MEDLINE]

>

>Lorene: You can get MBP and brain antibodies done at Immunosciences in

>Beverly Hills, CA but I don't know about getting it here from Edinburgh

>(we'll be doing a miniDAN! at University of Edinburgh in May, BTW).

>I'm not

>sure what " endothelium proteins " refers to, or what " retalted " means -

>please enlighten! Dr. JM

>

>

>