SV: new topic: Serpula lacrymans QPCR

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Ole Carlson, Mycoteam as

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Fra: Ole Carlson

Sendt: 26. november 2010 16:05

Til: iequality <iequality >

Emne: SV: new topic: Serpula lacrymans QPCR

Thanks for the information Bjørn !

Good to see that your methods are up and running !

I most definetly agree that mycologist should be well paid :-)

See you in Stochholm next week on the swesiaq conference 2 - 3 desember maybe ?

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Fra: Björn Mälarstig

Sendt: 25. november 2010 16:56

Til: iequality <iequality >

Emne: SV: new topic: Serpula lacrymans QPCR

Hi Ole and all others!

We are using Real-time-PCR since 2008 for detection of Serpula lacrymans on a

regular basis. We have also been using traditional microscope methods for 16

years. So I think we can tell the difference.

If you can see spores or typical hyphens you can easily say that this is Serpula

lacrymans. But if you only have decayed wood? If so the PCR is an excellent

tool. You can tell witch part of the infested construction that has other fungi

for instance Coniophora putana.

When it comes to sample taking the QPCR have some other advantages. It is seldom

we have an educated mycologist out taking the samples. Sometimes it’s even the

house owner herself taking the samples. Then you can use swabs to pat on the

fungi and then send the swab to the lab. If it is positive expertise can be sent

out. It is also a fast method, the turn around can be 48 h. It is cheaper than

traditional analyzes. Experienced mycologists are and shall be well-paid people.

We have made a validation study on the QPCR-method:

You do not even have to find the fungi. You can analyse the house dust. We have

a 93% certainty for dust tests. Sometimes it seems that the fragments of the

fungi not are transported in to the living areas. So in 7% of the cases we could

not detect DNA in the house dust when you had a growing S. lacrymans in the

crawlspace. But f you find considerably amount of DNA from Serpula lacrymans (>

10/ cm2) there is a 100% probability that you have the fungi in the house.

We had also a 100% correlation between known S. lacrymans and QPCR in material

samples. We had no false positive.

We have this analyze available together with our other microbiological analyses

for indoor air problems. We are also open for cooperation with our colleagues in

the laboratory business. QPCR-analyzes is cheap in large numbers and easy to

send by mail.

Vänliga hälsningar/Mit freundlichen Grüßen/Best regards

Björn Mälarstig

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Box 15120

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SE-750 15 UPPSALA

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Från: iequality [mailto:iequality ] För Ole

Carlson

Skickat: den 24 november 2010 17:49

Till: iequality

Ämne: SV: new topic: Serpula lacrymans

Tony ,

Good points about sampling,

But why do DNA when every half disent microscopist with some background in

mycology would reckognise the spores from their shape, size and colour in a

nonviable sampling (Air-O-cell or similair..)

Two of my former colleges have moved on and completed their phd on this

organism, and they have fascinating results using different dna- methods , but

in everyday life the good old microscope is still a lot faster and just as

certain.

When you detect an outbrake of serp lac we here in norway recomend removing of

all visibly infested wood material with a safety sone of 1 meter....removing of

infested mortar Can be expensive when the end of the beam is where it all starts

(and often underneath the newly renovated bathroom..).

Serp lac is the ONLY wood decaying fungus that we recomend using chemicals on in

buildings. Because of its ability to grow back ...

Med vennlig hilsen

Ole Carlson, Mycoteam as

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Mob: 952 38 931

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Fra: Havics

Sendt: 24. november 2010 17:11

Til: iequality <iequality >

Emne: new topic: Serpula lacrymans

Armour:

1. The spores require moisture. But if they germinate (on wood - or

stored paper or cardboard), that can create a hyphael highway from the water

source to elsewhere to feast upon wood.

2. Serpula lacrymans is also know to grow on mortar.

3. rate of spread

Winter 2.25 mm/day (0.75 m/yr)

In the Lab 4 m/year

Optimum temp 22C

Rapid growth as low as 5C

No apparent growth at 0C

No growth above 26C (it is apparently very heat sensitive)

4. And now a short story:

In 1812 the botanist Sowerby was asked to inspect the Queen Charlotte.

This ship, a 110 gun ship, was launched in 1810. It rotted so quickly that

is was necessary to rebuild the ship before she could be commissioned. The

cause - Boletus lacrymans (an early name for Serpula lacrymans).

So it is apparently the speed of growth combined with " infrequent " ability

to transport water elsewhere that makes this fungus (a basidiomycete) so

infamous.

Ref:

Coggins: Decay of Timber in Buildings, Dry Rot, Wet Rot and other Fungi.

Rentokil Ltd, 1980.

5. As for:

Doing viable air sample tests for Serp lac

Response:

Hah, hah, hah. (no offense Pat)

I'd like to see someone that can consistently get basidiomycetes to grow and

fruit in the lab.

This could actually be a nice application for PCR (if one could correlate

it).

6. I have seen it in older buildings and even once growing as

stalactites in a basement room (incredible image.

Tony

.......................................................................

" Tony " Havics, CHMM, CIH, PE

pH2, LLC

5250 E US 36, Suite 830 Avon

IN 46123

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