SV: new topic: Serpula lacrymans QPCR

[Guest guest]

Hi Ole and all others!

We are using Real-time-PCR since 2008 for detection of

Serpula lacrymans on a regular basis. We have also been using traditional

microscope methods for 16 years. So I think we can tell the difference.

If you can see spores or typical hyphens you can

easily say that this is Serpula lacrymans. But if you only have decayed wood? If

so the PCR is an excellent tool. You can tell witch part of the infested

construction that has other fungi for instance Coniophora

putana.

When it comes to sample taking the QPCR have some

other advantages. It is seldom we have an educated mycologist out taking the

samples. Sometimes it’s even the house owner herself taking the samples.  Then

you can use swabs to pat on the fungi and then send the swab to the lab. If it

is positive expertise can be sent out. It is also a fast method, the turn

around can be 48 h. It is cheaper than traditional analyzes. Experienced

mycologists are and shall be well-paid people.  

We have made a validation study on the QPCR-method:

You do not even have to find the fungi. You can

analyse the house dust. We have a 93% certainty for dust tests. Sometimes it

seems that the fragments of the fungi not are transported in to the living areas.

So in 7% of the cases we could not detect DNA in the house dust when you had a growing

S. lacrymans in the crawlspace.  But f you find considerably amount of DNA from

Serpula lacrymans (> 10/ cm2) there is a 100% probability that you have the

fungi in the house.

We had also a 100% correlation between known S.

lacrymans and QPCR in material samples. We had no false positive.

 

We have this analyze available together with our other

microbiological analyses for indoor air problems. We are also open for cooperation

with our colleagues in the laboratory business. QPCR-analyzes is cheap in large

numbers and easy to send by mail.

Vänliga hälsningar/Mit freundlichen Grüßen/Best

regards

 

Björn Mälarstig

 

anoZona

Box 15120

Vallongatan 1

SE-750 15 UPPSALA

SWEDEN

* tfn +46 18 444 43 41

* mobil +46708 15 55 02

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* bjorn.malarstig@...

www.anoZona.com

-----Ursprungligt meddelande-----

Från: iequality [mailto:iequality ] För Ole

Carlson

Skickat: den 24 november 2010 17:49

Till: iequality

Ämne: SV: new topic: Serpula lacrymans

Tony ,

Good points about sampling,

But why do DNA when every half disent microscopist with some background

in mycology would reckognise the spores from their shape, size and colour in a

nonviable sampling (Air-O-cell or similair..)

Two of my former colleges have moved on and completed their phd on this

organism, and they have fascinating results using different dna- methods , but

in everyday life the good old microscope is still a lot faster and just as

certain.

When you detect an outbrake of serp lac we here in norway recomend

removing of all visibly infested wood material with a safety sone of 1 meter....removing

of infested mortar Can be expensive when the end of the beam is where it all

starts (and often underneath the newly renovated bathroom..).

Serp lac is the ONLY wood decaying fungus that we recomend using

chemicals on in buildings. Because of its ability to grow back ...

Med vennlig hilsen

Ole Carlson, Mycoteam as

oec@...

Mob: 952 38 931

Sendt fra min HTC

________________________________

Fra: Havics

Sendt: 24. november 2010 17:11

Til: iequality <iequality >

Emne: new topic: Serpula lacrymans

Armour:

1. The spores require moisture. But if they germinate (on wood - or

stored paper or cardboard), that can create a hyphael highway from the

water

source to elsewhere to feast upon wood.

2. Serpula lacrymans is also know to grow on mortar.

3. rate of spread

Winter 2.25 mm/day (0.75 m/yr)

In the Lab 4 m/year

Optimum temp 22C

Rapid growth as low as 5C

No apparent growth at 0C

No growth above 26C (it is apparently very heat sensitive)

4. And now a short story:

In 1812 the botanist Sowerby was asked to inspect the Queen

Charlotte.

This ship, a 110 gun ship, was launched in 1810. It rotted so quickly

that

is was necessary to rebuild the ship before she could be commissioned.

The

cause - Boletus lacrymans (an early name for Serpula lacrymans).

So it is apparently the speed of growth combined with

" infrequent " ability

to transport water elsewhere that makes this fungus (a basidiomycete)

so

infamous.

Ref:

Coggins: Decay of Timber in Buildings, Dry Rot, Wet Rot and other

Fungi.

Rentokil Ltd, 1980.

5. As for:

Doing viable air sample tests for Serp lac

Response:

Hah, hah, hah. (no offense Pat)

I'd like to see someone that can consistently get basidiomycetes to

grow and

fruit in the lab.

This could actually be a nice application for PCR (if one could

correlate

it).

6. I have seen it in older buildings and even once growing as

stalactites in a basement room (incredible image.

Tony

.......................................................................

" Tony " Havics, CHMM, CIH, PE

pH2, LLC

5250 E US 36, Suite 830 Avon

IN 46123

www.ph2llc.com

off

fax

cell

90% of Risk Management is knowing where to place the decimal

point...any

consultant can give you the other 10%(SM)

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