Expression of Basic Fibroblast Growth Factor Results in the Decrease of Myostati

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Acta Biochim Biophys Sin (Shanghai). 2006 Oct;38(10):697-703.

Expression of Basic Fibroblast Growth Factor Results in the Decrease

of Myostatin mRNA in Murine C2C12 Myoblasts.

Liu HZ, Li Q, Yang XY, Liu L, Liu L, An XR, Chen YF.

State Key Laboratory for Agrobiotechnology, College of Biological

Science, China Agricultural University, Beijing 100094, China.

During the development and regeneration of skeletal muscle, many

growth factors, such as basic fibroblast growth factor (bFGF, FGF-2)

and myostatin, have been shown to play regulating roles. bFGF

contributes to promote proliferation and to inhibit differentiation

of skeletal muscle, whereas myostatin plays a series of contrasting

roles.

In order to elucidate whether the expression of bFGF has any

relationship with the expression of myostatin in skeletal muscle

cells, we constructed a eukaryotic expression vector for the

expression of exogenous bFGF in murine C2C12 myoblasts. Quantitative

RT-PCR assays indicated that with the increase of the expression of

exogenous bFGF gene, the expression of endogenous myostatin gene was

suppressed at mRNA level and protein level.