mic on kt

[Guest guest]

hi,

i would like to know how to do a minimal inhibition concentration test on

kombucha...pls let me know..how much of the kombucha precipitate do i add?...

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[Guest guest]

> i would like to know how to do a minimal

> inhibition concentration test on kombucha...

> pls let me know..how much of the kombucha

> precipitate do i add?...

I'm curious to know what kind of experiment you're trying to perform.

Knowing that, I could help you design an appropriate protocol.

You say " kombucha precipitate " . Are you centrifuging the kombucha to

come at the suspended microbiota?

Also, it'd be difficult to create an assay that would measure MIC on a

mixed colony such as kombucha, as any substance you'd test would

affect the various species differently. You'd probably need to test

each sample in quadruplicate for pH and sugar content. I'd recommend

(for ease of use) simple pH strips for pH measurement and an acid

hydrolysis-Benedict's solution test for sugar. That way you could

measure both sugar->alcohol (fungal) fermentation and alcohol->acid

(bacterial) fermentation.

I'd test on freshly-inoculated sweet tea, as well: the

pellet/precipiate from a volume of kombucha resuspended in its

original volume of white vinegar and nine volumes sweet tea. You'd

have to run one or two vinegar/tea without inoculant controls, of

course, to test initial acidity and sugar content.

If you have access to more in the way of microbiological supplies, you

could do colony counts rather than chemical tests, which would give

you a much more accurate MIC of your test substance, but would require

more effort. Still doable in a home setting, but more work.