[NVIC] Covering Up MMR/Autism Link

[Guest guest]

National Vaccine Information Center Newsletter

e-NEWS

October 5, 2006, 2006

MMR

" These data are a direct refutation of the reports of persistence of

measles virus in the tissues of autistic children, " he [ Ward, M.D.]

said. " We are hopeful that this paper will simply put a quiet end to the

debate surrounding this topic, " the researcher added. " - Reuters Health

Barbara Loe Fisher Commentary:

It is understandable why a member of the Canadian Infectious Disease

Society, who is an ardent promoter of forced vaccination with multiple

vaccines, would want to do whatever he could to exonerate MMR vaccine from

any association with autism. But does he really think his pathetic attempt

to discredit the meticulous work of virologists and microbiologists in

other laboratories will " simply put a quiet end to the debate " about MMR

and autism? I don't think so.

How tiresome it is becoming to see yet another M.D./Ph.D. vaccinologist (or

a group of them) issue a proclamation declaring that THEY have studied the

reported link between vaccines and autism and found absolutely no

association and, therefore, no more research should conducted and the

debate should " end. " This kind of posturing only furthers public suspicion

that they are covering up to protect the vaccine market and the mass

casualties of their one-size-fits-all mass vaccination policies.

Calls for a moratorium on further scientific investigation into the causes

and cures for any disease or disorder is reason to suspect that those who

are calling for it are afraid of the truth.

New Data Refute Measles Virus Persistence in Children With Autism

Reuters Health

Medscape

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(registration required)

October 2, 2006

By Rauscher

NEW YORK (Reuters Health) Oct 02 - Molecular studies by a Canadian team

show no evidence for the persistence of measles virus following MMR

immunization in peripheral blood mononuclear cells (PBMCs) of children with

autism spectrum disorder.

Dr. J. Ward and colleagues from Montreal's McGill University report

their findings in a paper in the October issue of Pediatrics. In it, they

note that despite mounting epidemiologic evidence against an association

between MMR vaccination and autism, several molecular investigations have

been used to implicate MMR vaccination in the development of autism

spectrum disorder in at least a subset of affected children.

For example, in 2000, using nested RT-PCR, Kawashima et al reported the

presence in PBMCs of one or more vaccine-strain measles virus gene in three

of nine children with autism compared with none of eight healthy children.

In another study, in 2002, Uhlmann et al using real-time PCR reported the

presence of measles virus fusion and hemagglutinin genes in biopsies from

62 of 68 autistic children compared with 4 of 39 biopsies from control

children.

Dr. Ward's team attempted but failed to replicate the results reported by

Kawashima and Uhlmann by applying their primer pairs to PBMCs isolated from

children with autism spectrum disorder and developmentally normal controls.

" In our hands, the primer-pairs published by Drs Kawashima and Uhlmann

yielded many PCR 'positive' results that turned out to be false-positive on

closer examination, " Dr. Ward told Reuters Health.

" These data are a direct refutation of the reports of persistence of

measles virus in the tissues of autistic children, " he said.

" We are hopeful that this paper will simply put a quiet end to the debate

surrounding this topic, " the researcher added.

PEDIATRICS Vol. 118 No. 4 October 2006, pp. 1664- 1675

(doi:10.1542/peds.2006-1262)

No Evidence of Persisting Measles Virus in Peripheral Blood Mononuclear

Cells From Children With Autism Spectrum Disorder

ABSTRACT

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OBJECTIVES. Despite epidemiologic evidence to the contrary, claims of an

association between measles- mumps-rubella vaccination and the development

of autism have persisted. Such claims are based primarily on the

identification of measles virus nucleic acids in tissues and body fluids by

polymerase chain reaction. We sought to determine whether measles virus

nucleic acids persist in children with autism spectrum disorder compared

with control children.

PATIENTS AND METHODS. Peripheral blood mononuclear cells were isolated from

54 children with autism spectrum disorder and 34 developmentally normal

children, and up to 4 real-time polymerase chain reaction assays and 2

nested polymerase chain reaction assays were performed. These assays

targeted the nucleoprotein, fusion, and hemagglutinin genes of measles

virus using previously published primer pairs with detection by SYBR green

I. Our own real-time assay targeted the fusion gene using novel primers and

an internal fluorescent probe. Positive reactions were evaluated

rigorously, and amplicons were sequenced. Finally, anti-measles antibody

titers were measured by enzyme immunoassay.

RESULTS. The real-time assays based on previously published primers gave

rise to a large number of positive reactions in both autism spectrum

disorder and control samples. Almost all of the positive reactions in these

assays were eliminated by evaluation of melting curves and amplicon band

size. The amplicons for the remaining positive reactions were cloned and

sequenced. No sample from either autism spectrum disorder or control groups

was found to contain nucleic acids from any measles virus gene. In the

nested polymerase chain reaction and in-house assays, none of the samples

yielded positive results. Furthermore, there was no difference in

anti-measles antibody titers between the autism and control groups.

INTERPRETATION. There is no evidence of measles virus persistence in the

peripheral blood mononuclear cells of children with autism spectrum disorder.

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Sheri Nakken, R.N., MA, Hahnemannian Homeopath

Vaccination Information & Choice Network, Nevada City CA & Wales UK

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